Larger muscle fibers and fiber bundles manifest smaller elastic modulus in paraspinal muscles of rats and humans

Abstract The passive elastic modulus of muscle fiber appears to be size-dependent. The objectives of this study were to determine whether this size effect was evident in the mechanical testing of muscle fiber bundles and to examine whether the muscle fiber bundle cross-section is circular. Muscle fi...

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Autores principales:	Masoud Malakoutian, Marine Theret, Shun Yamamoto, Iraj Dehghan-Hamani, Michael Lee, John Street, Fabio Rossi, Stephen H. M. Brown, Thomas R. Oxland
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ab724b5d2a4b4020b39f2cda4348405a
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spelling	oai:doaj.org-article:ab724b5d2a4b4020b39f2cda4348405a2021-12-02T15:15:44ZLarger muscle fibers and fiber bundles manifest smaller elastic modulus in paraspinal muscles of rats and humans10.1038/s41598-021-97895-z2045-2322https://doaj.org/article/ab724b5d2a4b4020b39f2cda4348405a2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97895-zhttps://doaj.org/toc/2045-2322Abstract The passive elastic modulus of muscle fiber appears to be size-dependent. The objectives of this study were to determine whether this size effect was evident in the mechanical testing of muscle fiber bundles and to examine whether the muscle fiber bundle cross-section is circular. Muscle fibers and fiber bundles were extracted from lumbar spine multifidus and longissimus of three cohorts: group one (G1) and two (G2) included 13 (330 ± 14 g) and 6 (452 ± 28 g) rats, while Group 3 (G3) comprised 9 degenerative spine patients. A minimum of six muscle fibers and six muscle fiber bundles from each muscle underwent cumulative stretches, each of 10% strain followed by 4 minutes relaxation. For all specimens, top and side diameters were measured. Elastic modulus was calculated as tangent at 30% strain from the stress–strain curve. Linear correlations between the sample cross sectional area (CSA) and elastic moduli in each group were performed. The correlations showed that increasing specimen CSA resulted in lower elastic modulus for both rats and humans, muscle fibers and fiber bundles. The median ratio of major to minor axis exceeded 1.0 for all groups, ranging between 1.15–1.29 for fibers and 1.27–1.44 for bundles. The lower elastic moduli with increasing size can be explained by relatively less collagenous extracellular matrix in the large fiber bundles. Future studies of passive property measurement should aim for consistent bundle sizes and measuring diameters of two orthogonal axes of the muscle specimens.Masoud MalakoutianMarine TheretShun YamamotoIraj Dehghan-HamaniMichael LeeJohn StreetFabio RossiStephen H. M. BrownThomas R. OxlandNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Masoud Malakoutian Marine Theret Shun Yamamoto Iraj Dehghan-Hamani Michael Lee John Street Fabio Rossi Stephen H. M. Brown Thomas R. Oxland Larger muscle fibers and fiber bundles manifest smaller elastic modulus in paraspinal muscles of rats and humans
description	Abstract The passive elastic modulus of muscle fiber appears to be size-dependent. The objectives of this study were to determine whether this size effect was evident in the mechanical testing of muscle fiber bundles and to examine whether the muscle fiber bundle cross-section is circular. Muscle fibers and fiber bundles were extracted from lumbar spine multifidus and longissimus of three cohorts: group one (G1) and two (G2) included 13 (330 ± 14 g) and 6 (452 ± 28 g) rats, while Group 3 (G3) comprised 9 degenerative spine patients. A minimum of six muscle fibers and six muscle fiber bundles from each muscle underwent cumulative stretches, each of 10% strain followed by 4 minutes relaxation. For all specimens, top and side diameters were measured. Elastic modulus was calculated as tangent at 30% strain from the stress–strain curve. Linear correlations between the sample cross sectional area (CSA) and elastic moduli in each group were performed. The correlations showed that increasing specimen CSA resulted in lower elastic modulus for both rats and humans, muscle fibers and fiber bundles. The median ratio of major to minor axis exceeded 1.0 for all groups, ranging between 1.15–1.29 for fibers and 1.27–1.44 for bundles. The lower elastic moduli with increasing size can be explained by relatively less collagenous extracellular matrix in the large fiber bundles. Future studies of passive property measurement should aim for consistent bundle sizes and measuring diameters of two orthogonal axes of the muscle specimens.
format	article
author	Masoud Malakoutian Marine Theret Shun Yamamoto Iraj Dehghan-Hamani Michael Lee John Street Fabio Rossi Stephen H. M. Brown Thomas R. Oxland
author_facet	Masoud Malakoutian Marine Theret Shun Yamamoto Iraj Dehghan-Hamani Michael Lee John Street Fabio Rossi Stephen H. M. Brown Thomas R. Oxland
author_sort	Masoud Malakoutian
title	Larger muscle fibers and fiber bundles manifest smaller elastic modulus in paraspinal muscles of rats and humans
title_short	Larger muscle fibers and fiber bundles manifest smaller elastic modulus in paraspinal muscles of rats and humans
title_full	Larger muscle fibers and fiber bundles manifest smaller elastic modulus in paraspinal muscles of rats and humans
title_fullStr	Larger muscle fibers and fiber bundles manifest smaller elastic modulus in paraspinal muscles of rats and humans
title_full_unstemmed	Larger muscle fibers and fiber bundles manifest smaller elastic modulus in paraspinal muscles of rats and humans
title_sort	larger muscle fibers and fiber bundles manifest smaller elastic modulus in paraspinal muscles of rats and humans
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ab724b5d2a4b4020b39f2cda4348405a
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Larger muscle fibers and fiber bundles manifest smaller elastic modulus in paraspinal muscles of rats and humans

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