Rheological analysis of sputum from patients with chronic bronchial diseases

Abstract Bronchial diseases are characterised by the weak efficiency of mucus transport through the lower airways, leading in some cases to the muco-obstruction of bronchi. It has been hypothesised that this loss of clearance results from alterations in the mucus rheology, which are reflected in spu...

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Autores principales:	Jérémy Patarin, Étienne Ghiringhelli, Guillaume Darsy, Martinien Obamba, Philippe Bochu, Boubou Camara, Sébastien Quétant, Jean-Luc Cracowski, Claire Cracowski, Matthieu Robert de Saint Vincent
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ab7d3437aa40470a8d0e091185068c0d
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spelling	oai:doaj.org-article:ab7d3437aa40470a8d0e091185068c0d2021-12-02T18:48:01ZRheological analysis of sputum from patients with chronic bronchial diseases10.1038/s41598-020-72672-62045-2322https://doaj.org/article/ab7d3437aa40470a8d0e091185068c0d2020-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-72672-6https://doaj.org/toc/2045-2322Abstract Bronchial diseases are characterised by the weak efficiency of mucus transport through the lower airways, leading in some cases to the muco-obstruction of bronchi. It has been hypothesised that this loss of clearance results from alterations in the mucus rheology, which are reflected in sputum samples collected from patients, making sputum rheology a possible biophysical marker of these diseases and their evolution. However, previous rheological studies have focused on quasi-static viscoelastic (linear storage and loss moduli) properties only, which are not representative of the mucus mobilisation within the respiratory tract. In this paper, we extend this approach further, by analysing both quasi-static and some dynamic (flow point) properties, to assess their usability and relative performance in characterising several chronic bronchial diseases (asthma, chronic obstructive pulmonary disease, and cystic fibrosis) and distinguishing them from healthy subjects. We demonstrate that pathologies influence substantially the linear and flow properties. Linear moduli are weakly condition-specific and even though the corresponding ranges overlap, distinct levels can be identified. This directly relates to the specific mucus structure in each case. In contrast, the flow point is found to strongly increase in muco-obstructive diseases, which may reflect the complete failure of mucociliary clearance causing episodic obstructions. These results suggest that the analysis of quasi-static and dynamic regimes in sputum rheology is in fact useful as these regimes provide complementary markers of chronic bronchial diseases.Jérémy PatarinÉtienne GhiringhelliGuillaume DarsyMartinien ObambaPhilippe BochuBoubou CamaraSébastien QuétantJean-Luc CracowskiClaire CracowskiMatthieu Robert de Saint VincentNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jérémy Patarin Étienne Ghiringhelli Guillaume Darsy Martinien Obamba Philippe Bochu Boubou Camara Sébastien Quétant Jean-Luc Cracowski Claire Cracowski Matthieu Robert de Saint Vincent Rheological analysis of sputum from patients with chronic bronchial diseases
description	Abstract Bronchial diseases are characterised by the weak efficiency of mucus transport through the lower airways, leading in some cases to the muco-obstruction of bronchi. It has been hypothesised that this loss of clearance results from alterations in the mucus rheology, which are reflected in sputum samples collected from patients, making sputum rheology a possible biophysical marker of these diseases and their evolution. However, previous rheological studies have focused on quasi-static viscoelastic (linear storage and loss moduli) properties only, which are not representative of the mucus mobilisation within the respiratory tract. In this paper, we extend this approach further, by analysing both quasi-static and some dynamic (flow point) properties, to assess their usability and relative performance in characterising several chronic bronchial diseases (asthma, chronic obstructive pulmonary disease, and cystic fibrosis) and distinguishing them from healthy subjects. We demonstrate that pathologies influence substantially the linear and flow properties. Linear moduli are weakly condition-specific and even though the corresponding ranges overlap, distinct levels can be identified. This directly relates to the specific mucus structure in each case. In contrast, the flow point is found to strongly increase in muco-obstructive diseases, which may reflect the complete failure of mucociliary clearance causing episodic obstructions. These results suggest that the analysis of quasi-static and dynamic regimes in sputum rheology is in fact useful as these regimes provide complementary markers of chronic bronchial diseases.
format	article
author	Jérémy Patarin Étienne Ghiringhelli Guillaume Darsy Martinien Obamba Philippe Bochu Boubou Camara Sébastien Quétant Jean-Luc Cracowski Claire Cracowski Matthieu Robert de Saint Vincent
author_facet	Jérémy Patarin Étienne Ghiringhelli Guillaume Darsy Martinien Obamba Philippe Bochu Boubou Camara Sébastien Quétant Jean-Luc Cracowski Claire Cracowski Matthieu Robert de Saint Vincent
author_sort	Jérémy Patarin
title	Rheological analysis of sputum from patients with chronic bronchial diseases
title_short	Rheological analysis of sputum from patients with chronic bronchial diseases
title_full	Rheological analysis of sputum from patients with chronic bronchial diseases
title_fullStr	Rheological analysis of sputum from patients with chronic bronchial diseases
title_full_unstemmed	Rheological analysis of sputum from patients with chronic bronchial diseases
title_sort	rheological analysis of sputum from patients with chronic bronchial diseases
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/ab7d3437aa40470a8d0e091185068c0d
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Rheological analysis of sputum from patients with chronic bronchial diseases

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