Botryococcus braunii as a bioreactor for the production of nanoparticles with antimicrobial potentialities

Alejandra Arévalo-Gallegos,1 J Saul Garcia-Perez,1 Danay Carrillo-Nieves,1 RA Ramirez-Mendoza,1 Hafiz MN Iqbal,1 Roberto Parra-Saldívar1–4 1Tecnologico de Monterrey, Escuela de Ingenieria y Ciencias, Campus Monterrey, Monterrey, Nuevo León, Mexico; 2Micros...

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Autores principales:	Arévalo-Gallegos A, Garcia-Perez JS, Carrillo-Nieves D, Ramirez-Mendoza RA, Iqbal HMN, Parra-Saldívar R
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Biosynthesis Microalgae Silver nanoparticles Antimicrobial activity Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ab8cb653f6f147a9962a9326a1349b9e
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spelling	oai:doaj.org-article:ab8cb653f6f147a9962a9326a1349b9e2021-12-02T07:56:33ZBotryococcus braunii as a bioreactor for the production of nanoparticles with antimicrobial potentialities1178-2013https://doaj.org/article/ab8cb653f6f147a9962a9326a1349b9e2018-09-01T00:00:00Zhttps://www.dovepress.com/botryococcus-braunii-as-a-bioreactor-for-the-production-of-nanoparticl-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Alejandra Arévalo-Gallegos,1 J Saul Garcia-Perez,1 Danay Carrillo-Nieves,1 RA Ramirez-Mendoza,1 Hafiz MN Iqbal,1 Roberto Parra-Saldívar1–4 1Tecnologico de Monterrey, Escuela de Ingenieria y Ciencias, Campus Monterrey, Monterrey, Nuevo León, Mexico; 2Microsystems Technologies Laboratories, Massachusetts Institute of Technology, Cambridge, MA, USA; 3Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Cambridge, MA, USA; 4Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, USA Background: Microalgae produce metabolites with notable potentialities to act as reducing and capping agents for the synthesis of silver nanoparticles (AgNPs) in a process widely recognized as an eco-friendly and cheaper alternative for the generation of nanoparticles (NPs). Methods: In the present work, AgNPs were synthesized using live Botryococcus braunii cultures. Two biosynthesis routes were explored: (1) intracellular and (2) extracellular at pH levels of 6–9 using 1–5 mM silver nitrate concentrations. Results: The generation of NPs was confirmed via ultraviolet–visible spectroscopy. The morphological characteristics were observed using scanning electron microscopy which revealed that the newly developed AgNPs were mostly spherical in sizes starting from 168 nm. The characteristic peaks in a typical Fourier transform infrared spectroscopy suggested that the exopolysaccharides were the possible reducing and capping agents. The antimicrobial spectrum of the newly developed AgNPs was tested against bacterial strains, both Gram-negative, Gram-positive, and yeast, ie, Escherichia coli (American Type Culture Collection [ATCC] 25922), Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 25923), and the yeast Candida albicans (ATCC 10231), respectively. The antimicrobial activity tests showed a stronger inhibition against Gram-negative bacteria. Statistically, the NPs biosynthesized at pH values of 6 and 8 displayed a higher antimicrobial activity. Conclusion: Our findings showed that B. braunii is capable of generating AgNPs with antimicrobial potential. Keywords: biosynthesis, microalgae, silver nanoparticles, antimicrobial activityArévalo-Gallegos AGarcia-Perez JSCarrillo-Nieves DRamirez-Mendoza RAIqbal HMNParra-Saldívar RDove Medical PressarticleBiosynthesisMicroalgaeSilver nanoparticlesAntimicrobial activityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 5591-5604 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biosynthesis Microalgae Silver nanoparticles Antimicrobial activity Medicine (General) R5-920
spellingShingle	Biosynthesis Microalgae Silver nanoparticles Antimicrobial activity Medicine (General) R5-920 Arévalo-Gallegos A Garcia-Perez JS Carrillo-Nieves D Ramirez-Mendoza RA Iqbal HMN Parra-Saldívar R Botryococcus braunii as a bioreactor for the production of nanoparticles with antimicrobial potentialities
description	Alejandra Arévalo-Gallegos,1 J Saul Garcia-Perez,1 Danay Carrillo-Nieves,1 RA Ramirez-Mendoza,1 Hafiz MN Iqbal,1 Roberto Parra-Saldívar1–4 1Tecnologico de Monterrey, Escuela de Ingenieria y Ciencias, Campus Monterrey, Monterrey, Nuevo León, Mexico; 2Microsystems Technologies Laboratories, Massachusetts Institute of Technology, Cambridge, MA, USA; 3Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Cambridge, MA, USA; 4Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, USA Background: Microalgae produce metabolites with notable potentialities to act as reducing and capping agents for the synthesis of silver nanoparticles (AgNPs) in a process widely recognized as an eco-friendly and cheaper alternative for the generation of nanoparticles (NPs). Methods: In the present work, AgNPs were synthesized using live Botryococcus braunii cultures. Two biosynthesis routes were explored: (1) intracellular and (2) extracellular at pH levels of 6–9 using 1–5 mM silver nitrate concentrations. Results: The generation of NPs was confirmed via ultraviolet–visible spectroscopy. The morphological characteristics were observed using scanning electron microscopy which revealed that the newly developed AgNPs were mostly spherical in sizes starting from 168 nm. The characteristic peaks in a typical Fourier transform infrared spectroscopy suggested that the exopolysaccharides were the possible reducing and capping agents. The antimicrobial spectrum of the newly developed AgNPs was tested against bacterial strains, both Gram-negative, Gram-positive, and yeast, ie, Escherichia coli (American Type Culture Collection [ATCC] 25922), Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 25923), and the yeast Candida albicans (ATCC 10231), respectively. The antimicrobial activity tests showed a stronger inhibition against Gram-negative bacteria. Statistically, the NPs biosynthesized at pH values of 6 and 8 displayed a higher antimicrobial activity. Conclusion: Our findings showed that B. braunii is capable of generating AgNPs with antimicrobial potential. Keywords: biosynthesis, microalgae, silver nanoparticles, antimicrobial activity
format	article
author	Arévalo-Gallegos A Garcia-Perez JS Carrillo-Nieves D Ramirez-Mendoza RA Iqbal HMN Parra-Saldívar R
author_facet	Arévalo-Gallegos A Garcia-Perez JS Carrillo-Nieves D Ramirez-Mendoza RA Iqbal HMN Parra-Saldívar R
author_sort	Arévalo-Gallegos A
title	Botryococcus braunii as a bioreactor for the production of nanoparticles with antimicrobial potentialities
title_short	Botryococcus braunii as a bioreactor for the production of nanoparticles with antimicrobial potentialities
title_full	Botryococcus braunii as a bioreactor for the production of nanoparticles with antimicrobial potentialities
title_fullStr	Botryococcus braunii as a bioreactor for the production of nanoparticles with antimicrobial potentialities
title_full_unstemmed	Botryococcus braunii as a bioreactor for the production of nanoparticles with antimicrobial potentialities
title_sort	botryococcus braunii as a bioreactor for the production of nanoparticles with antimicrobial potentialities
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/ab8cb653f6f147a9962a9326a1349b9e
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Botryococcus braunii as a bioreactor for the production of nanoparticles with antimicrobial potentialities

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