Investigating Extracellular DNA Release in Staphylococcus xylosus Biofilm In Vitro

Staphylococcus xylosus forms biofilm embedded in an extracellular polymeric matrix. As extracellular DNA (eDNA) resulting from cell lysis has been found in several staphylococcal biofilms, we investigated S. xylosus biofilm in vitro by a microscopic approach and...

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Autores principales:	Sabine Leroy, Isabelle Lebert, Carine Andant, Pierre Micheau, Régine Talon
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Staphylococcus xylosus</i> biofilm eDNA cell lysis amino sugar catabolism DNA/RNA repair Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ab9c1a86ada74bc8b580dac3b77b5148
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id	oai:doaj.org-article:ab9c1a86ada74bc8b580dac3b77b5148
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spelling	oai:doaj.org-article:ab9c1a86ada74bc8b580dac3b77b51482021-11-25T18:24:07ZInvestigating Extracellular DNA Release in <i>Staphylococcus xylosus</i> Biofilm In Vitro10.3390/microorganisms91121922076-2607https://doaj.org/article/ab9c1a86ada74bc8b580dac3b77b51482021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2192https://doaj.org/toc/2076-2607<i>Staphylococcus xylosus</i> forms biofilm embedded in an extracellular polymeric matrix. As extracellular DNA (eDNA) resulting from cell lysis has been found in several staphylococcal biofilms, we investigated <i>S. xylosus</i> biofilm in vitro by a microscopic approach and identified the mechanisms involved in cell lysis by a transcriptomic approach. Confocal laser scanning microscopy (CLSM) analyses of the biofilms, together with DNA staining and DNase treatment, revealed that eDNA constituted an important component of the matrix. This eDNA resulted from cell lysis by two mechanisms, overexpression of phage-related genes and of <i>cidABC</i> encoding a holin protein that is an effector of murein hydrolase activity. This lysis might furnish nutrients for the remaining cells as highlighted by genes overexpressed in nucleotide salvage, in amino sugar catabolism and in inorganic ion transports. Several genes involved in DNA/RNA repair and genes encoding proteases and chaperones involved in protein turnover were up-regulated. Furthermore, <i>S. xylosus</i> perceived osmotic and oxidative stresses and responded by up-regulating genes involved in osmoprotectant synthesis and in detoxification. This study provides new insight into the physiology of <i>S. xylosus</i> in biofilm.Sabine LeroyIsabelle LebertCarine AndantPierre MicheauRégine TalonMDPI AGarticle<i>Staphylococcus xylosus</i>biofilmeDNAcell lysisamino sugar catabolismDNA/RNA repairBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2192, p 2192 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Staphylococcus xylosus</i> biofilm eDNA cell lysis amino sugar catabolism DNA/RNA repair Biology (General) QH301-705.5
spellingShingle	<i>Staphylococcus xylosus</i> biofilm eDNA cell lysis amino sugar catabolism DNA/RNA repair Biology (General) QH301-705.5 Sabine Leroy Isabelle Lebert Carine Andant Pierre Micheau Régine Talon Investigating Extracellular DNA Release in <i>Staphylococcus xylosus</i> Biofilm In Vitro
description	<i>Staphylococcus xylosus</i> forms biofilm embedded in an extracellular polymeric matrix. As extracellular DNA (eDNA) resulting from cell lysis has been found in several staphylococcal biofilms, we investigated <i>S. xylosus</i> biofilm in vitro by a microscopic approach and identified the mechanisms involved in cell lysis by a transcriptomic approach. Confocal laser scanning microscopy (CLSM) analyses of the biofilms, together with DNA staining and DNase treatment, revealed that eDNA constituted an important component of the matrix. This eDNA resulted from cell lysis by two mechanisms, overexpression of phage-related genes and of <i>cidABC</i> encoding a holin protein that is an effector of murein hydrolase activity. This lysis might furnish nutrients for the remaining cells as highlighted by genes overexpressed in nucleotide salvage, in amino sugar catabolism and in inorganic ion transports. Several genes involved in DNA/RNA repair and genes encoding proteases and chaperones involved in protein turnover were up-regulated. Furthermore, <i>S. xylosus</i> perceived osmotic and oxidative stresses and responded by up-regulating genes involved in osmoprotectant synthesis and in detoxification. This study provides new insight into the physiology of <i>S. xylosus</i> in biofilm.
format	article
author	Sabine Leroy Isabelle Lebert Carine Andant Pierre Micheau Régine Talon
author_facet	Sabine Leroy Isabelle Lebert Carine Andant Pierre Micheau Régine Talon
author_sort	Sabine Leroy
title	Investigating Extracellular DNA Release in <i>Staphylococcus xylosus</i> Biofilm In Vitro
title_short	Investigating Extracellular DNA Release in <i>Staphylococcus xylosus</i> Biofilm In Vitro
title_full	Investigating Extracellular DNA Release in <i>Staphylococcus xylosus</i> Biofilm In Vitro
title_fullStr	Investigating Extracellular DNA Release in <i>Staphylococcus xylosus</i> Biofilm In Vitro
title_full_unstemmed	Investigating Extracellular DNA Release in <i>Staphylococcus xylosus</i> Biofilm In Vitro
title_sort	investigating extracellular dna release in <i>staphylococcus xylosus</i> biofilm in vitro
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ab9c1a86ada74bc8b580dac3b77b5148
work_keys_str_mv	AT sabineleroy investigatingextracellulardnareleaseinistaphylococcusxylosusibiofilminvitro AT isabellelebert investigatingextracellulardnareleaseinistaphylococcusxylosusibiofilminvitro AT carineandant investigatingextracellulardnareleaseinistaphylococcusxylosusibiofilminvitro AT pierremicheau investigatingextracellulardnareleaseinistaphylococcusxylosusibiofilminvitro AT reginetalon investigatingextracellulardnareleaseinistaphylococcusxylosusibiofilminvitro
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Investigating Extracellular DNA Release in <i>Staphylococcus xylosus</i> Biofilm In Vitro

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