LncRNA FGD5-AS1 functions as an oncogene to upregulate GTPBP4 expression by sponging miR-873-5p in hepatocellular carcinoma

The long non-coding FGD5-AS1 (LncFGD5-AS1) has been reported to be a novel carcinogenic gene and participant in regulating tumor progression by sponging microRNAs (miRNAs). However, the pattern of expression and the biological role of FGD5-AS1 in hepatocellular carcinoma (HCC) remains largely unkno...

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Autores principales:	Nuobei Zhang, Hao Shen, Shenan Huang, Fenfen Wang, Huifang Liu, Fen Xie, Lei Jiang, Xin Chen
Formato:	article
Lenguaje:	EN
Publicado:	PAGEPress Publications 2021
Materias:	Hepatocellular carcinoma FGD5-AS1 miR-873-5p GTPBP4 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/aba31fe3b7a944a5b44f019aa68d98ec
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spelling	oai:doaj.org-article:aba31fe3b7a944a5b44f019aa68d98ec2021-11-17T07:41:24ZLncRNA FGD5-AS1 functions as an oncogene to upregulate GTPBP4 expression by sponging miR-873-5p in hepatocellular carcinoma 10.4081/ejh.2021.33001121-760X2038-8306https://doaj.org/article/aba31fe3b7a944a5b44f019aa68d98ec2021-11-01T00:00:00Zhttps://ejh.it/index.php/ejh/article/view/3300https://doaj.org/toc/1121-760Xhttps://doaj.org/toc/2038-8306 The long non-coding FGD5-AS1 (LncFGD5-AS1) has been reported to be a novel carcinogenic gene and participant in regulating tumor progression by sponging microRNAs (miRNAs). However, the pattern of expression and the biological role of FGD5-AS1 in hepatocellular carcinoma (HCC) remains largely unknown. The expression level of FGD5-AS1 in tumor tissues and cell lines was measured by RT-qPCR. CCK-8, EdU, flow cytometry, wound healing, and transwell chamber assays were performed to investigate the role of FGD5-AS1 in cell proliferation, apoptosis, migration, and invasion in HCC. Dual luciferase reporter, and RNA pull-down assays were performed to identify the regulatory interactions among FGD5-AS1, miR-873-5p and GTP-binding protein 4 (GTPBP4). We found that the expression of FGD5-AS1 was upregulated in HCC tissues and cell lines. Moreover, the knockdown of FGD5-AS1 suppressed cell proliferation, migration and invasion, and induced apoptosis in HCC cells. Further studies demonstrated that FGD5-AS1 could function as a competitive RNA by sponging miR-873-5p in HCC cells. Moreover, GTPBP4 was identified as direct downstream target of miR-873-5p in HCC cells and FGD5-AS1mediated the effects of GTPBP4 by competitively binding with miR-873-5p. Taken together, this study demonstrated the regulatory role of FGD5-AS1 in the progression of HCC and identified the miR-873-5p/GTPBP4 axis as the direct downstream pathway. It represents a promising novel therapeutic strategy for HCC patients. Nuobei ZhangHao ShenShenan HuangFenfen WangHuifang LiuFen XieLei JiangXin ChenPAGEPress PublicationsarticleHepatocellular carcinomaFGD5-AS1miR-873-5pGTPBP4Biology (General)QH301-705.5ENEuropean Journal of Histochemistry , Vol 65, Iss 4 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Hepatocellular carcinoma FGD5-AS1 miR-873-5p GTPBP4 Biology (General) QH301-705.5
spellingShingle	Hepatocellular carcinoma FGD5-AS1 miR-873-5p GTPBP4 Biology (General) QH301-705.5 Nuobei Zhang Hao Shen Shenan Huang Fenfen Wang Huifang Liu Fen Xie Lei Jiang Xin Chen LncRNA FGD5-AS1 functions as an oncogene to upregulate GTPBP4 expression by sponging miR-873-5p in hepatocellular carcinoma
description	The long non-coding FGD5-AS1 (LncFGD5-AS1) has been reported to be a novel carcinogenic gene and participant in regulating tumor progression by sponging microRNAs (miRNAs). However, the pattern of expression and the biological role of FGD5-AS1 in hepatocellular carcinoma (HCC) remains largely unknown. The expression level of FGD5-AS1 in tumor tissues and cell lines was measured by RT-qPCR. CCK-8, EdU, flow cytometry, wound healing, and transwell chamber assays were performed to investigate the role of FGD5-AS1 in cell proliferation, apoptosis, migration, and invasion in HCC. Dual luciferase reporter, and RNA pull-down assays were performed to identify the regulatory interactions among FGD5-AS1, miR-873-5p and GTP-binding protein 4 (GTPBP4). We found that the expression of FGD5-AS1 was upregulated in HCC tissues and cell lines. Moreover, the knockdown of FGD5-AS1 suppressed cell proliferation, migration and invasion, and induced apoptosis in HCC cells. Further studies demonstrated that FGD5-AS1 could function as a competitive RNA by sponging miR-873-5p in HCC cells. Moreover, GTPBP4 was identified as direct downstream target of miR-873-5p in HCC cells and FGD5-AS1mediated the effects of GTPBP4 by competitively binding with miR-873-5p. Taken together, this study demonstrated the regulatory role of FGD5-AS1 in the progression of HCC and identified the miR-873-5p/GTPBP4 axis as the direct downstream pathway. It represents a promising novel therapeutic strategy for HCC patients.
format	article
author	Nuobei Zhang Hao Shen Shenan Huang Fenfen Wang Huifang Liu Fen Xie Lei Jiang Xin Chen
author_facet	Nuobei Zhang Hao Shen Shenan Huang Fenfen Wang Huifang Liu Fen Xie Lei Jiang Xin Chen
author_sort	Nuobei Zhang
title	LncRNA FGD5-AS1 functions as an oncogene to upregulate GTPBP4 expression by sponging miR-873-5p in hepatocellular carcinoma
title_short	LncRNA FGD5-AS1 functions as an oncogene to upregulate GTPBP4 expression by sponging miR-873-5p in hepatocellular carcinoma
title_full	LncRNA FGD5-AS1 functions as an oncogene to upregulate GTPBP4 expression by sponging miR-873-5p in hepatocellular carcinoma
title_fullStr	LncRNA FGD5-AS1 functions as an oncogene to upregulate GTPBP4 expression by sponging miR-873-5p in hepatocellular carcinoma
title_full_unstemmed	LncRNA FGD5-AS1 functions as an oncogene to upregulate GTPBP4 expression by sponging miR-873-5p in hepatocellular carcinoma
title_sort	lncrna fgd5-as1 functions as an oncogene to upregulate gtpbp4 expression by sponging mir-873-5p in hepatocellular carcinoma
publisher	PAGEPress Publications
publishDate	2021
url	https://doaj.org/article/aba31fe3b7a944a5b44f019aa68d98ec
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LncRNA FGD5-AS1 functions as an oncogene to upregulate GTPBP4 expression by sponging miR-873-5p in hepatocellular carcinoma

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