Enhanced HIFU Theranostics with Dual-Frequency-Ring Focused Ultrasound and Activatable Perfluoropentane-Loaded Polymer Nanoparticles

High-intensity focused ultrasound (HIFU) has been widely used in tumor ablation in clinical settings. Meanwhile, there is great potential to increase the therapeutic efficiency of temporary cavitation due to enhanced thermal effects and combined mechanical effects from nonlinear vibration and collap...

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Autores principales:	Junjie Chen, Zhezhu Nan, Yubo Zhao, Lei Zhang, Hongrui Zhu, Daocheng Wu, Yujin Zong, Mingzhu Lu, Tali Ilovitsh, Mingxi Wan, Kai Yan, Yi Feng
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	high-intensity focused ultrasound (HIFU) cavitation theranostics dual frequency perfluoropentane-loaded polymer nanoparticle (PFP@Polymer NP) Mechanical engineering and machinery TJ1-1570
Acceso en línea:	https://doaj.org/article/abaa6c9251a34040b5864a3f8294b30e
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spelling	oai:doaj.org-article:abaa6c9251a34040b5864a3f8294b30e2021-11-25T18:23:08ZEnhanced HIFU Theranostics with Dual-Frequency-Ring Focused Ultrasound and Activatable Perfluoropentane-Loaded Polymer Nanoparticles10.3390/mi121113242072-666Xhttps://doaj.org/article/abaa6c9251a34040b5864a3f8294b30e2021-10-01T00:00:00Zhttps://www.mdpi.com/2072-666X/12/11/1324https://doaj.org/toc/2072-666XHigh-intensity focused ultrasound (HIFU) has been widely used in tumor ablation in clinical settings. Meanwhile, there is great potential to increase the therapeutic efficiency of temporary cavitation due to enhanced thermal effects and combined mechanical effects from nonlinear vibration and collapse of the microbubbles. In this study, dual-frequency (1.1 and 5 MHz) HIFU was used to produce acoustic droplet vaporization (ADV) microbubbles from activatable perfluoropentane-loaded polymer nanoparticles (PFP@Polymer NPs), which increased the therapeutic outcome of the HIFU and helped realize tumor theranostics with ultrasound contrast imaging. Combined with PFP@Polymer NPs, dual-frequency HIFU changed the shape of the damage lesion and reduced the acoustic intensity threshold of thermal damage significantly, from 216.86 to 62.38 W/cm<sup>2</sup>. It produced a nearly 20 °C temperature increase in half the irradiation time and exhibited a higher tumor inhibition rate (84.5% ± 3.4%) at a low acoustic intensity (1.1 MHz: 23.77 W/cm<sup>2</sup>; 5 MHz: 0.35 W/cm<sup>2</sup>) in vitro than the single-frequency HIFU (60.2% ± 11.9%). Moreover, compared with the traditional PFP@BSA NDs, PFP@Polymer NPs showed higher anti-tumor efficacy (81.13% vs. 69.34%; * <i>p</i> < 0.05) and better contrast-enhanced ultrasound (CEUS) imaging ability (gray value of 57.53 vs. 30.67; **** <i>p</i> < 0.0001), probably benefitting from its uniform and stable structure. It showed potential as a highly efficient tumor theranostics approach based on dual-frequency HIFU and activatable PFP@Polymer NPs.Junjie ChenZhezhu NanYubo ZhaoLei ZhangHongrui ZhuDaocheng WuYujin ZongMingzhu LuTali IlovitshMingxi WanKai YanYi FengMDPI AGarticlehigh-intensity focused ultrasound (HIFU)cavitationtheranosticsdual frequencyperfluoropentane-loaded polymer nanoparticle (PFP@Polymer NP)Mechanical engineering and machineryTJ1-1570ENMicromachines, Vol 12, Iss 1324, p 1324 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	high-intensity focused ultrasound (HIFU) cavitation theranostics dual frequency perfluoropentane-loaded polymer nanoparticle (PFP@Polymer NP) Mechanical engineering and machinery TJ1-1570
spellingShingle	high-intensity focused ultrasound (HIFU) cavitation theranostics dual frequency perfluoropentane-loaded polymer nanoparticle (PFP@Polymer NP) Mechanical engineering and machinery TJ1-1570 Junjie Chen Zhezhu Nan Yubo Zhao Lei Zhang Hongrui Zhu Daocheng Wu Yujin Zong Mingzhu Lu Tali Ilovitsh Mingxi Wan Kai Yan Yi Feng Enhanced HIFU Theranostics with Dual-Frequency-Ring Focused Ultrasound and Activatable Perfluoropentane-Loaded Polymer Nanoparticles
description	High-intensity focused ultrasound (HIFU) has been widely used in tumor ablation in clinical settings. Meanwhile, there is great potential to increase the therapeutic efficiency of temporary cavitation due to enhanced thermal effects and combined mechanical effects from nonlinear vibration and collapse of the microbubbles. In this study, dual-frequency (1.1 and 5 MHz) HIFU was used to produce acoustic droplet vaporization (ADV) microbubbles from activatable perfluoropentane-loaded polymer nanoparticles (PFP@Polymer NPs), which increased the therapeutic outcome of the HIFU and helped realize tumor theranostics with ultrasound contrast imaging. Combined with PFP@Polymer NPs, dual-frequency HIFU changed the shape of the damage lesion and reduced the acoustic intensity threshold of thermal damage significantly, from 216.86 to 62.38 W/cm<sup>2</sup>. It produced a nearly 20 °C temperature increase in half the irradiation time and exhibited a higher tumor inhibition rate (84.5% ± 3.4%) at a low acoustic intensity (1.1 MHz: 23.77 W/cm<sup>2</sup>; 5 MHz: 0.35 W/cm<sup>2</sup>) in vitro than the single-frequency HIFU (60.2% ± 11.9%). Moreover, compared with the traditional PFP@BSA NDs, PFP@Polymer NPs showed higher anti-tumor efficacy (81.13% vs. 69.34%; * <i>p</i> < 0.05) and better contrast-enhanced ultrasound (CEUS) imaging ability (gray value of 57.53 vs. 30.67; **** <i>p</i> < 0.0001), probably benefitting from its uniform and stable structure. It showed potential as a highly efficient tumor theranostics approach based on dual-frequency HIFU and activatable PFP@Polymer NPs.
format	article
author	Junjie Chen Zhezhu Nan Yubo Zhao Lei Zhang Hongrui Zhu Daocheng Wu Yujin Zong Mingzhu Lu Tali Ilovitsh Mingxi Wan Kai Yan Yi Feng
author_facet	Junjie Chen Zhezhu Nan Yubo Zhao Lei Zhang Hongrui Zhu Daocheng Wu Yujin Zong Mingzhu Lu Tali Ilovitsh Mingxi Wan Kai Yan Yi Feng
author_sort	Junjie Chen
title	Enhanced HIFU Theranostics with Dual-Frequency-Ring Focused Ultrasound and Activatable Perfluoropentane-Loaded Polymer Nanoparticles
title_short	Enhanced HIFU Theranostics with Dual-Frequency-Ring Focused Ultrasound and Activatable Perfluoropentane-Loaded Polymer Nanoparticles
title_full	Enhanced HIFU Theranostics with Dual-Frequency-Ring Focused Ultrasound and Activatable Perfluoropentane-Loaded Polymer Nanoparticles
title_fullStr	Enhanced HIFU Theranostics with Dual-Frequency-Ring Focused Ultrasound and Activatable Perfluoropentane-Loaded Polymer Nanoparticles
title_full_unstemmed	Enhanced HIFU Theranostics with Dual-Frequency-Ring Focused Ultrasound and Activatable Perfluoropentane-Loaded Polymer Nanoparticles
title_sort	enhanced hifu theranostics with dual-frequency-ring focused ultrasound and activatable perfluoropentane-loaded polymer nanoparticles
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/abaa6c9251a34040b5864a3f8294b30e
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Enhanced HIFU Theranostics with Dual-Frequency-Ring Focused Ultrasound and Activatable Perfluoropentane-Loaded Polymer Nanoparticles

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