Comparison of Two Diagnostic Assays for Anti-Laminin 332 Mucous Membrane Pemphigoid

Anti-laminin 332 mucous membrane pemphigoid (MMP) is an autoimmune blistering disease characterized by predominant mucosal lesions and autoantibodies against laminin 332. The exact diagnosis of anti-laminin 332 MMP is important since nearly 30% of patients develop solid cancers. This study compared...

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Autores principales:	Stephanie Goletz, Federica Giurdanella, Maike M. Holtsche, Miranda Nijenhuis, Barbara Horvath, Gilles F. H. Diercks, Detlef Zillikens, Takashi Hashimoto, Enno Schmidt, Hendri H. Pas
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	autoantibody blistering biochip cancer footprint laminin 332 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/ababc5e370bd45299b68ae0f420b8693
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spelling	oai:doaj.org-article:ababc5e370bd45299b68ae0f420b86932021-12-01T02:55:42ZComparison of Two Diagnostic Assays for Anti-Laminin 332 Mucous Membrane Pemphigoid1664-322410.3389/fimmu.2021.773720https://doaj.org/article/ababc5e370bd45299b68ae0f420b86932021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.773720/fullhttps://doaj.org/toc/1664-3224Anti-laminin 332 mucous membrane pemphigoid (MMP) is an autoimmune blistering disease characterized by predominant mucosal lesions and autoantibodies against laminin 332. The exact diagnosis of anti-laminin 332 MMP is important since nearly 30% of patients develop solid cancers. This study compared two independently developed diagnostic indirect immunofluorescence (IF) tests based on recombinant laminin 332 expressed in HEK239 cells (biochip mosaic assay) and the migration trails of cultured keratinocytes rich in laminin 332 (footprint assay). The sera of 54 anti-laminin 332 MMP, 35 non-anti-laminin 332 MMP, and 30 pemphigus vulgaris patients as well as 20 healthy blood donors were analyzed blindly and independently. Fifty-two of 54 and 54/54 anti-laminin 332 MMP sera were positive in the biochip mosaic and the footprint assay, respectively. In the 35 non-anti-laminin 332 MMP sera, 3 were positive in both tests and 4 others showed weak reactivity in the footprint assay. In conclusion, both assays are easy to perform, highly sensitive, and specific, which will further facilitate the diagnosis of anti-laminin 332 MMP.Stephanie GoletzFederica GiurdanellaMaike M. HoltscheMiranda NijenhuisBarbara HorvathGilles F. H. DiercksDetlef ZillikensTakashi HashimotoEnno SchmidtEnno SchmidtHendri H. PasFrontiers Media S.A.articleautoantibodyblisteringbiochipcancerfootprintlaminin 332Immunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	autoantibody blistering biochip cancer footprint laminin 332 Immunologic diseases. Allergy RC581-607
spellingShingle	autoantibody blistering biochip cancer footprint laminin 332 Immunologic diseases. Allergy RC581-607 Stephanie Goletz Federica Giurdanella Maike M. Holtsche Miranda Nijenhuis Barbara Horvath Gilles F. H. Diercks Detlef Zillikens Takashi Hashimoto Enno Schmidt Enno Schmidt Hendri H. Pas Comparison of Two Diagnostic Assays for Anti-Laminin 332 Mucous Membrane Pemphigoid
description	Anti-laminin 332 mucous membrane pemphigoid (MMP) is an autoimmune blistering disease characterized by predominant mucosal lesions and autoantibodies against laminin 332. The exact diagnosis of anti-laminin 332 MMP is important since nearly 30% of patients develop solid cancers. This study compared two independently developed diagnostic indirect immunofluorescence (IF) tests based on recombinant laminin 332 expressed in HEK239 cells (biochip mosaic assay) and the migration trails of cultured keratinocytes rich in laminin 332 (footprint assay). The sera of 54 anti-laminin 332 MMP, 35 non-anti-laminin 332 MMP, and 30 pemphigus vulgaris patients as well as 20 healthy blood donors were analyzed blindly and independently. Fifty-two of 54 and 54/54 anti-laminin 332 MMP sera were positive in the biochip mosaic and the footprint assay, respectively. In the 35 non-anti-laminin 332 MMP sera, 3 were positive in both tests and 4 others showed weak reactivity in the footprint assay. In conclusion, both assays are easy to perform, highly sensitive, and specific, which will further facilitate the diagnosis of anti-laminin 332 MMP.
format	article
author	Stephanie Goletz Federica Giurdanella Maike M. Holtsche Miranda Nijenhuis Barbara Horvath Gilles F. H. Diercks Detlef Zillikens Takashi Hashimoto Enno Schmidt Enno Schmidt Hendri H. Pas
author_facet	Stephanie Goletz Federica Giurdanella Maike M. Holtsche Miranda Nijenhuis Barbara Horvath Gilles F. H. Diercks Detlef Zillikens Takashi Hashimoto Enno Schmidt Enno Schmidt Hendri H. Pas
author_sort	Stephanie Goletz
title	Comparison of Two Diagnostic Assays for Anti-Laminin 332 Mucous Membrane Pemphigoid
title_short	Comparison of Two Diagnostic Assays for Anti-Laminin 332 Mucous Membrane Pemphigoid
title_full	Comparison of Two Diagnostic Assays for Anti-Laminin 332 Mucous Membrane Pemphigoid
title_fullStr	Comparison of Two Diagnostic Assays for Anti-Laminin 332 Mucous Membrane Pemphigoid
title_full_unstemmed	Comparison of Two Diagnostic Assays for Anti-Laminin 332 Mucous Membrane Pemphigoid
title_sort	comparison of two diagnostic assays for anti-laminin 332 mucous membrane pemphigoid
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/ababc5e370bd45299b68ae0f420b8693
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Comparison of Two Diagnostic Assays for Anti-Laminin 332 Mucous Membrane Pemphigoid

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