Profiling circulating microRNA expression in experimental sepsis using cecal ligation and puncture.
The levels of circulating microRNAs (miRNAs) in mice with experimental sepsis induced by cecal ligation and puncture (CLP) were determined using whole blood samples obtained from C57BL/6 mice at 4, 8, and 24 h after CLP; miRNA expression analysis was performed in these samples using an miRNA array....
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Public Library of Science (PLoS)
2013
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oai:doaj.org-article:abad7a8b855f4266bafe36688614104a2021-11-18T08:48:56ZProfiling circulating microRNA expression in experimental sepsis using cecal ligation and puncture.1932-620310.1371/journal.pone.0077936https://doaj.org/article/abad7a8b855f4266bafe36688614104a2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24205035/?tool=EBIhttps://doaj.org/toc/1932-6203The levels of circulating microRNAs (miRNAs) in mice with experimental sepsis induced by cecal ligation and puncture (CLP) were determined using whole blood samples obtained from C57BL/6 mice at 4, 8, and 24 h after CLP; miRNA expression analysis was performed in these samples using an miRNA array. Microarray analysis revealed upregulation of 10 miRNA targets (miR-16, miR-17, miR-20a, miR-20b, miR-26a, miR-26b, miR-106a, miR-106b, miR-195, and miR-451). The expression of these miRNA targets in the whole blood, serum, and white blood cells (WBCs) of CLP mice was quantified using quantitative real-time PCR; these values were compared to those in sham-operated C57BL/6 mice, and the results indicated that these miRNA targets were significantly up-regulated in the whole blood and serum but not in the WBCs. In addition, the levels of these 10 miRNA targets in the serum of Tlr2-/-, Tlr4-/-, and NF-κB-/- mice at 8 h after CLP did not decrease significantly., which indicated that the transcription of these miRNAs was not directly mediated by the TLR2/NF-κB or TLR4/NF-κB pathway, and pathways induced by exposure to the gram-positive or gram-negative bacteria. Immunoprecipitation with the Argonaute 2 ribonucleoprotein complex revealed significantly increased expression of the 10 miRNA targets in the serum of mice after CLP, and the levels of 6 (miR-16, miR-17, miR-20a, miR-20b, miR-26a, and miR-26b) of these 10 miRNA targets increased significantly in exosomes isolated using ExoQuick precipitation solution. In this study, we identified circulating miRNAs that were up-regulated after CLP and determined the increase in the levels of these miRNAs, and our results suggest that circulating Ago2 complexes and exosomes may be responsible for the stability of miRNAs in the serum.Shao-Chun WuJohnson Chia-Shen YangCheng-Shyuan RauYi-Chun ChenTsu-Hsiang LuMing-Wei LinSiou-Ling TzengYi-Chan WuChia-Jung WuChing-Hua HsiehPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 10, p e77936 (2013) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Shao-Chun Wu Johnson Chia-Shen Yang Cheng-Shyuan Rau Yi-Chun Chen Tsu-Hsiang Lu Ming-Wei Lin Siou-Ling Tzeng Yi-Chan Wu Chia-Jung Wu Ching-Hua Hsieh Profiling circulating microRNA expression in experimental sepsis using cecal ligation and puncture. |
| description |
The levels of circulating microRNAs (miRNAs) in mice with experimental sepsis induced by cecal ligation and puncture (CLP) were determined using whole blood samples obtained from C57BL/6 mice at 4, 8, and 24 h after CLP; miRNA expression analysis was performed in these samples using an miRNA array. Microarray analysis revealed upregulation of 10 miRNA targets (miR-16, miR-17, miR-20a, miR-20b, miR-26a, miR-26b, miR-106a, miR-106b, miR-195, and miR-451). The expression of these miRNA targets in the whole blood, serum, and white blood cells (WBCs) of CLP mice was quantified using quantitative real-time PCR; these values were compared to those in sham-operated C57BL/6 mice, and the results indicated that these miRNA targets were significantly up-regulated in the whole blood and serum but not in the WBCs. In addition, the levels of these 10 miRNA targets in the serum of Tlr2-/-, Tlr4-/-, and NF-κB-/- mice at 8 h after CLP did not decrease significantly., which indicated that the transcription of these miRNAs was not directly mediated by the TLR2/NF-κB or TLR4/NF-κB pathway, and pathways induced by exposure to the gram-positive or gram-negative bacteria. Immunoprecipitation with the Argonaute 2 ribonucleoprotein complex revealed significantly increased expression of the 10 miRNA targets in the serum of mice after CLP, and the levels of 6 (miR-16, miR-17, miR-20a, miR-20b, miR-26a, and miR-26b) of these 10 miRNA targets increased significantly in exosomes isolated using ExoQuick precipitation solution. In this study, we identified circulating miRNAs that were up-regulated after CLP and determined the increase in the levels of these miRNAs, and our results suggest that circulating Ago2 complexes and exosomes may be responsible for the stability of miRNAs in the serum. |
| format |
article |
| author |
Shao-Chun Wu Johnson Chia-Shen Yang Cheng-Shyuan Rau Yi-Chun Chen Tsu-Hsiang Lu Ming-Wei Lin Siou-Ling Tzeng Yi-Chan Wu Chia-Jung Wu Ching-Hua Hsieh |
| author_facet |
Shao-Chun Wu Johnson Chia-Shen Yang Cheng-Shyuan Rau Yi-Chun Chen Tsu-Hsiang Lu Ming-Wei Lin Siou-Ling Tzeng Yi-Chan Wu Chia-Jung Wu Ching-Hua Hsieh |
| author_sort |
Shao-Chun Wu |
| title |
Profiling circulating microRNA expression in experimental sepsis using cecal ligation and puncture. |
| title_short |
Profiling circulating microRNA expression in experimental sepsis using cecal ligation and puncture. |
| title_full |
Profiling circulating microRNA expression in experimental sepsis using cecal ligation and puncture. |
| title_fullStr |
Profiling circulating microRNA expression in experimental sepsis using cecal ligation and puncture. |
| title_full_unstemmed |
Profiling circulating microRNA expression in experimental sepsis using cecal ligation and puncture. |
| title_sort |
profiling circulating microrna expression in experimental sepsis using cecal ligation and puncture. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/abad7a8b855f4266bafe36688614104a |
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