Circulating Tumor Cell Clusters Are Cloaked with Platelets and Correlate with Poor Prognosis in Unresectable Pancreatic Cancer

Circulating tumor cells (CTCs) are known to be heterogeneous and clustered with tumor-associated cells, such as macrophages, neutrophils, fibroblasts, and platelets. However, their molecular profile and clinical significance remain largely unknown. Thus, we aimed to perform a comprehensive gene expr...

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Autores principales:	Minji Lim, Suhyun Park, Hyoung-Oh Jeong, Sung Hee Park, Sumit Kumar, Aelee Jang, Semin Lee, Dong Uk Kim, Yoon-Kyoung Cho
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	circulating tumor cells circulating tumor cell clusters pancreatic cancer platelets Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/abaf4af3d2cf4497b9755a07958d9f47
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spelling	oai:doaj.org-article:abaf4af3d2cf4497b9755a07958d9f472021-11-11T15:26:47ZCirculating Tumor Cell Clusters Are Cloaked with Platelets and Correlate with Poor Prognosis in Unresectable Pancreatic Cancer10.3390/cancers132152722072-6694https://doaj.org/article/abaf4af3d2cf4497b9755a07958d9f472021-10-01T00:00:00Zhttps://www.mdpi.com/2072-6694/13/21/5272https://doaj.org/toc/2072-6694Circulating tumor cells (CTCs) are known to be heterogeneous and clustered with tumor-associated cells, such as macrophages, neutrophils, fibroblasts, and platelets. However, their molecular profile and clinical significance remain largely unknown. Thus, we aimed to perform a comprehensive gene expression analysis of single CTCs and CTC clusters in patients with pancreatic cancer and to identify their potential clinical relevance to provide personalized medicine. Epitope-independent, rapid (>3 mL of whole blood/min) isolation of single CTCs and CTC clusters was achieved from a prospective cohort of 16 patients with unresectable pancreatic cancer using a centrifugal microfluidic device. Forty-eight mRNA expressions of individual CTCs and CTC clusters were analyzed to identify pancreatic CTC phenotype. CTC clusters had a larger proportion of mesenchymal expression than single CTCs (<i>p</i> = 0.0004). The presence of CTC clusters positively correlated with poor prognosis (progression-free survival, <i>p</i> = 0.0159; overall survival, <i>p</i> = 0.0186). Furthermore, we found that most CTCs in these patients (90.7%) were cloaked with platelets and found the presence of a positive correlation between the increase in CTC clusters and rapid disease progression during follow-ups. Efficient CTC cluster isolation and analysis techniques will enhance the understanding of complex tumor metastasis processes and can facilitate personalized disease management.Minji LimSuhyun ParkHyoung-Oh JeongSung Hee ParkSumit KumarAelee JangSemin LeeDong Uk KimYoon-Kyoung ChoMDPI AGarticlecirculating tumor cellscirculating tumor cell clusterspancreatic cancerplateletsNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancers, Vol 13, Iss 5272, p 5272 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	circulating tumor cells circulating tumor cell clusters pancreatic cancer platelets Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	circulating tumor cells circulating tumor cell clusters pancreatic cancer platelets Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Minji Lim Suhyun Park Hyoung-Oh Jeong Sung Hee Park Sumit Kumar Aelee Jang Semin Lee Dong Uk Kim Yoon-Kyoung Cho Circulating Tumor Cell Clusters Are Cloaked with Platelets and Correlate with Poor Prognosis in Unresectable Pancreatic Cancer
description	Circulating tumor cells (CTCs) are known to be heterogeneous and clustered with tumor-associated cells, such as macrophages, neutrophils, fibroblasts, and platelets. However, their molecular profile and clinical significance remain largely unknown. Thus, we aimed to perform a comprehensive gene expression analysis of single CTCs and CTC clusters in patients with pancreatic cancer and to identify their potential clinical relevance to provide personalized medicine. Epitope-independent, rapid (>3 mL of whole blood/min) isolation of single CTCs and CTC clusters was achieved from a prospective cohort of 16 patients with unresectable pancreatic cancer using a centrifugal microfluidic device. Forty-eight mRNA expressions of individual CTCs and CTC clusters were analyzed to identify pancreatic CTC phenotype. CTC clusters had a larger proportion of mesenchymal expression than single CTCs (<i>p</i> = 0.0004). The presence of CTC clusters positively correlated with poor prognosis (progression-free survival, <i>p</i> = 0.0159; overall survival, <i>p</i> = 0.0186). Furthermore, we found that most CTCs in these patients (90.7%) were cloaked with platelets and found the presence of a positive correlation between the increase in CTC clusters and rapid disease progression during follow-ups. Efficient CTC cluster isolation and analysis techniques will enhance the understanding of complex tumor metastasis processes and can facilitate personalized disease management.
format	article
author	Minji Lim Suhyun Park Hyoung-Oh Jeong Sung Hee Park Sumit Kumar Aelee Jang Semin Lee Dong Uk Kim Yoon-Kyoung Cho
author_facet	Minji Lim Suhyun Park Hyoung-Oh Jeong Sung Hee Park Sumit Kumar Aelee Jang Semin Lee Dong Uk Kim Yoon-Kyoung Cho
author_sort	Minji Lim
title	Circulating Tumor Cell Clusters Are Cloaked with Platelets and Correlate with Poor Prognosis in Unresectable Pancreatic Cancer
title_short	Circulating Tumor Cell Clusters Are Cloaked with Platelets and Correlate with Poor Prognosis in Unresectable Pancreatic Cancer
title_full	Circulating Tumor Cell Clusters Are Cloaked with Platelets and Correlate with Poor Prognosis in Unresectable Pancreatic Cancer
title_fullStr	Circulating Tumor Cell Clusters Are Cloaked with Platelets and Correlate with Poor Prognosis in Unresectable Pancreatic Cancer
title_full_unstemmed	Circulating Tumor Cell Clusters Are Cloaked with Platelets and Correlate with Poor Prognosis in Unresectable Pancreatic Cancer
title_sort	circulating tumor cell clusters are cloaked with platelets and correlate with poor prognosis in unresectable pancreatic cancer
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/abaf4af3d2cf4497b9755a07958d9f47
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_version_	1718435326890868736

Circulating Tumor Cell Clusters Are Cloaked with Platelets and Correlate with Poor Prognosis in Unresectable Pancreatic Cancer

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