Gut microbiota signature of pathogen-dependent dysbiosis in viral gastroenteritis

Abstract Acute gastroenteritis associated with diarrhea is considered a serious disease in Africa and South Asia. In this study, we examined the trends in the causative pathogens of diarrhea and the corresponding gut microbiota in Ghana using microbiome analysis performed on diarrheic stools via 16S...

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Autores principales:	Taketoshi Mizutani, Samuel Yaw Aboagye, Aya Ishizaka, Theophillus Afum, Gloria Ivy Mensah, Adwoa Asante-Poku, Diana Asema Asandem, Prince Kofi Parbie, Christopher Zaab-Yen Abana, Dennis Kushitor, Evelyn Yayra Bonney, Motoi Adachi, Hiroki Hori, Koichi Ishikawa, Tetsuro Matano, Kiyosu Taniguchi, David Opare, Doris Arhin, Franklin Asiedu-Bekoe, William Kwabena Ampofo, Dorothy Yeboah-Manu, Kwadwo Ansah Koram, Abraham Kwabena Anang, Hiroshi Kiyono
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/abb2715808744f9a87b8c6f9257ebabc
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spelling	oai:doaj.org-article:abb2715808744f9a87b8c6f9257ebabc2021-12-02T15:39:58ZGut microbiota signature of pathogen-dependent dysbiosis in viral gastroenteritis10.1038/s41598-021-93345-y2045-2322https://doaj.org/article/abb2715808744f9a87b8c6f9257ebabc2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93345-yhttps://doaj.org/toc/2045-2322Abstract Acute gastroenteritis associated with diarrhea is considered a serious disease in Africa and South Asia. In this study, we examined the trends in the causative pathogens of diarrhea and the corresponding gut microbiota in Ghana using microbiome analysis performed on diarrheic stools via 16S rRNA sequencing. In total, 80 patients with diarrhea and 34 healthy adults as controls, from 2017 to 2018, were enrolled in the study. Among the patients with diarrhea, 39 were norovirus-positive and 18 were rotavirus-positive. The analysis of species richness (Chao1) was lower in patients with diarrhea than that in controls. Beta-diversity analysis revealed significant differences between the two groups. Several diarrhea-related pathogens (e.g., Escherichia-Shigella, Klebsiella and Campylobacter) were detected in patients with diarrhea. Furthermore, co-infection with these pathogens and enteroviruses (e.g., norovirus and rotavirus) was observed in several cases. Levels of both Erysipelotrichaceae and Staphylococcaceae family markedly differed between norovirus-positive and -negative diarrheic stools, and the 10 predicted metabolic pathways, including the carbohydrate metabolism pathway, showed significant differences between rotavirus-positive patients with diarrhea and controls. This comparative study of diarrheal pathogens in Ghana revealed specific trends in the gut microbiota signature associated with diarrhea and that pathogen-dependent dysbiosis occurred in viral gastroenteritis.Taketoshi MizutaniSamuel Yaw AboagyeAya IshizakaTheophillus AfumGloria Ivy MensahAdwoa Asante-PokuDiana Asema AsandemPrince Kofi ParbieChristopher Zaab-Yen AbanaDennis KushitorEvelyn Yayra BonneyMotoi AdachiHiroki HoriKoichi IshikawaTetsuro MatanoKiyosu TaniguchiDavid OpareDoris ArhinFranklin Asiedu-BekoeWilliam Kwabena AmpofoDorothy Yeboah-ManuKwadwo Ansah KoramAbraham Kwabena AnangHiroshi KiyonoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Taketoshi Mizutani Samuel Yaw Aboagye Aya Ishizaka Theophillus Afum Gloria Ivy Mensah Adwoa Asante-Poku Diana Asema Asandem Prince Kofi Parbie Christopher Zaab-Yen Abana Dennis Kushitor Evelyn Yayra Bonney Motoi Adachi Hiroki Hori Koichi Ishikawa Tetsuro Matano Kiyosu Taniguchi David Opare Doris Arhin Franklin Asiedu-Bekoe William Kwabena Ampofo Dorothy Yeboah-Manu Kwadwo Ansah Koram Abraham Kwabena Anang Hiroshi Kiyono Gut microbiota signature of pathogen-dependent dysbiosis in viral gastroenteritis
description	Abstract Acute gastroenteritis associated with diarrhea is considered a serious disease in Africa and South Asia. In this study, we examined the trends in the causative pathogens of diarrhea and the corresponding gut microbiota in Ghana using microbiome analysis performed on diarrheic stools via 16S rRNA sequencing. In total, 80 patients with diarrhea and 34 healthy adults as controls, from 2017 to 2018, were enrolled in the study. Among the patients with diarrhea, 39 were norovirus-positive and 18 were rotavirus-positive. The analysis of species richness (Chao1) was lower in patients with diarrhea than that in controls. Beta-diversity analysis revealed significant differences between the two groups. Several diarrhea-related pathogens (e.g., Escherichia-Shigella, Klebsiella and Campylobacter) were detected in patients with diarrhea. Furthermore, co-infection with these pathogens and enteroviruses (e.g., norovirus and rotavirus) was observed in several cases. Levels of both Erysipelotrichaceae and Staphylococcaceae family markedly differed between norovirus-positive and -negative diarrheic stools, and the 10 predicted metabolic pathways, including the carbohydrate metabolism pathway, showed significant differences between rotavirus-positive patients with diarrhea and controls. This comparative study of diarrheal pathogens in Ghana revealed specific trends in the gut microbiota signature associated with diarrhea and that pathogen-dependent dysbiosis occurred in viral gastroenteritis.
format	article
author	Taketoshi Mizutani Samuel Yaw Aboagye Aya Ishizaka Theophillus Afum Gloria Ivy Mensah Adwoa Asante-Poku Diana Asema Asandem Prince Kofi Parbie Christopher Zaab-Yen Abana Dennis Kushitor Evelyn Yayra Bonney Motoi Adachi Hiroki Hori Koichi Ishikawa Tetsuro Matano Kiyosu Taniguchi David Opare Doris Arhin Franklin Asiedu-Bekoe William Kwabena Ampofo Dorothy Yeboah-Manu Kwadwo Ansah Koram Abraham Kwabena Anang Hiroshi Kiyono
author_facet	Taketoshi Mizutani Samuel Yaw Aboagye Aya Ishizaka Theophillus Afum Gloria Ivy Mensah Adwoa Asante-Poku Diana Asema Asandem Prince Kofi Parbie Christopher Zaab-Yen Abana Dennis Kushitor Evelyn Yayra Bonney Motoi Adachi Hiroki Hori Koichi Ishikawa Tetsuro Matano Kiyosu Taniguchi David Opare Doris Arhin Franklin Asiedu-Bekoe William Kwabena Ampofo Dorothy Yeboah-Manu Kwadwo Ansah Koram Abraham Kwabena Anang Hiroshi Kiyono
author_sort	Taketoshi Mizutani
title	Gut microbiota signature of pathogen-dependent dysbiosis in viral gastroenteritis
title_short	Gut microbiota signature of pathogen-dependent dysbiosis in viral gastroenteritis
title_full	Gut microbiota signature of pathogen-dependent dysbiosis in viral gastroenteritis
title_fullStr	Gut microbiota signature of pathogen-dependent dysbiosis in viral gastroenteritis
title_full_unstemmed	Gut microbiota signature of pathogen-dependent dysbiosis in viral gastroenteritis
title_sort	gut microbiota signature of pathogen-dependent dysbiosis in viral gastroenteritis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/abb2715808744f9a87b8c6f9257ebabc
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Gut microbiota signature of pathogen-dependent dysbiosis in viral gastroenteritis

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