Fatty Acid Uptake in T Cell Subsets Using a Quantum Dot Fatty Acid Conjugate

Abstract Fatty acid (FA) metabolism directly influences the functional capabilities of T cells in tumor microenvironments. Thus, developing tools to interrogate FA-uptake by T cell subsets is important for understanding tumor immunosuppression. Herein, we have generated a novel FA-Qdot 605 dye conju...

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Autores principales:	Megan E. Muroski, Jason Miska, Alan L. Chang, Peng Zhang, Aida Rashidi, Haley Moore, Aurora Lopez-Rosas, Yu Han, Maciej S. Lesniak
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/abb432fcf52848cdae2859e8d6b42e95
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spelling	oai:doaj.org-article:abb432fcf52848cdae2859e8d6b42e952021-12-02T15:05:24ZFatty Acid Uptake in T Cell Subsets Using a Quantum Dot Fatty Acid Conjugate10.1038/s41598-017-05556-x2045-2322https://doaj.org/article/abb432fcf52848cdae2859e8d6b42e952017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05556-xhttps://doaj.org/toc/2045-2322Abstract Fatty acid (FA) metabolism directly influences the functional capabilities of T cells in tumor microenvironments. Thus, developing tools to interrogate FA-uptake by T cell subsets is important for understanding tumor immunosuppression. Herein, we have generated a novel FA-Qdot 605 dye conjugate with superior sensitivity and flexibility to any of the previously commercially available alternatives. For the first time, we demonstrate that this nanoparticle can be used as a specific measure of fatty acid uptake by T cells both in-vitro and in-vivo. Flow cytometric analysis shows that both the location and activation status of T cells determines their FA uptake. Additionally, CD4+ Foxp3+ regulatory T cells (Tregs) uptake FA at a higher rate than effector T cell subsets, supporting the role of FA metabolism for Treg function. Furthermore, we are able to simultaneously detect glucose and fatty acid uptake directly within the tumor microenvironment. Cumulatively, our results suggest that this novel fluorescent probe is a powerful tool to understand FA utilization within the tumor, thereby providing an unprecedented opportunity to study T cell FA metabolism in-vivo.Megan E. MuroskiJason MiskaAlan L. ChangPeng ZhangAida RashidiHaley MooreAurora Lopez-RosasYu HanMaciej S. LesniakNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Megan E. Muroski Jason Miska Alan L. Chang Peng Zhang Aida Rashidi Haley Moore Aurora Lopez-Rosas Yu Han Maciej S. Lesniak Fatty Acid Uptake in T Cell Subsets Using a Quantum Dot Fatty Acid Conjugate
description	Abstract Fatty acid (FA) metabolism directly influences the functional capabilities of T cells in tumor microenvironments. Thus, developing tools to interrogate FA-uptake by T cell subsets is important for understanding tumor immunosuppression. Herein, we have generated a novel FA-Qdot 605 dye conjugate with superior sensitivity and flexibility to any of the previously commercially available alternatives. For the first time, we demonstrate that this nanoparticle can be used as a specific measure of fatty acid uptake by T cells both in-vitro and in-vivo. Flow cytometric analysis shows that both the location and activation status of T cells determines their FA uptake. Additionally, CD4+ Foxp3+ regulatory T cells (Tregs) uptake FA at a higher rate than effector T cell subsets, supporting the role of FA metabolism for Treg function. Furthermore, we are able to simultaneously detect glucose and fatty acid uptake directly within the tumor microenvironment. Cumulatively, our results suggest that this novel fluorescent probe is a powerful tool to understand FA utilization within the tumor, thereby providing an unprecedented opportunity to study T cell FA metabolism in-vivo.
format	article
author	Megan E. Muroski Jason Miska Alan L. Chang Peng Zhang Aida Rashidi Haley Moore Aurora Lopez-Rosas Yu Han Maciej S. Lesniak
author_facet	Megan E. Muroski Jason Miska Alan L. Chang Peng Zhang Aida Rashidi Haley Moore Aurora Lopez-Rosas Yu Han Maciej S. Lesniak
author_sort	Megan E. Muroski
title	Fatty Acid Uptake in T Cell Subsets Using a Quantum Dot Fatty Acid Conjugate
title_short	Fatty Acid Uptake in T Cell Subsets Using a Quantum Dot Fatty Acid Conjugate
title_full	Fatty Acid Uptake in T Cell Subsets Using a Quantum Dot Fatty Acid Conjugate
title_fullStr	Fatty Acid Uptake in T Cell Subsets Using a Quantum Dot Fatty Acid Conjugate
title_full_unstemmed	Fatty Acid Uptake in T Cell Subsets Using a Quantum Dot Fatty Acid Conjugate
title_sort	fatty acid uptake in t cell subsets using a quantum dot fatty acid conjugate
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/abb432fcf52848cdae2859e8d6b42e95
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Fatty Acid Uptake in T Cell Subsets Using a Quantum Dot Fatty Acid Conjugate

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