A Novel Approach to Characterize the Lipidome of Marine Archaeon Nitrosopumilus maritimus by Ion Mobility Mass Spectrometry

Archaea are differentiated from the other two domains of life by their biomolecular characteristics. One such characteristic is the unique structure and composition of their lipids. Characterization of the whole set of lipids in a biological system (the lipidome) remains technologically challenging....

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Autores principales:	Kai P. Law, Wei He, Jianchang Tao, Chuanlun Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	archaea thaumarchaeota Nitrosopumilus maritimus lipidomics ion mobility mass spectrometry Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/abb440dde33c46b38a715b213d247934
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spelling	oai:doaj.org-article:abb440dde33c46b38a715b213d2479342021-12-02T10:42:50ZA Novel Approach to Characterize the Lipidome of Marine Archaeon Nitrosopumilus maritimus by Ion Mobility Mass Spectrometry1664-302X10.3389/fmicb.2021.735878https://doaj.org/article/abb440dde33c46b38a715b213d2479342021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.735878/fullhttps://doaj.org/toc/1664-302XArchaea are differentiated from the other two domains of life by their biomolecular characteristics. One such characteristic is the unique structure and composition of their lipids. Characterization of the whole set of lipids in a biological system (the lipidome) remains technologically challenging. This is because the lipidome is innately complex, and not all lipid species are extractable, separable, or ionizable by a single analytical method. Furthermore, lipids are structurally and chemically diverse. Many lipids are isobaric or isomeric and often indistinguishable by the measurement of mass or even their fragmentation spectra. Here we developed a novel analytical protocol based on liquid chromatography ion mobility mass spectrometry to enhance the coverage of the lipidome and characterize the conformations of archaeal lipids by their collision cross-sections (CCSs). The measurements of ion mobility revealed the gas-phase ion chemistry of representative archaeal lipids and provided further insights into their attributions to the adaptability of archaea to environmental stresses. A comprehensive characterization of the lipidome of mesophilic marine thaumarchaeon, Nitrosopumilus maritimus (strain SCM1) revealed potentially an unreported phosphate- and sulfate-containing lipid candidate by negative ionization analysis. It was the first time that experimentally derived CCS values of archaeal lipids were reported. Discrimination of crenarchaeol and its proposed stereoisomer was, however, not achieved with the resolving power of the SYNAPT G2 ion mobility system, and a high-resolution ion mobility system may be required for future work. Structural and spectral libraries of archaeal lipids were constructed in non-vendor-specific formats and are being made available to the community to promote research of Archaea by lipidomics.Kai P. LawKai P. LawKai P. LawWei HeWei HeJianchang TaoJianchang TaoChuanlun ZhangChuanlun ZhangChuanlun ZhangFrontiers Media S.A.articlearchaeathaumarchaeotaNitrosopumilus maritimuslipidomicsion mobility mass spectrometryMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	archaea thaumarchaeota Nitrosopumilus maritimus lipidomics ion mobility mass spectrometry Microbiology QR1-502
spellingShingle	archaea thaumarchaeota Nitrosopumilus maritimus lipidomics ion mobility mass spectrometry Microbiology QR1-502 Kai P. Law Kai P. Law Kai P. Law Wei He Wei He Jianchang Tao Jianchang Tao Chuanlun Zhang Chuanlun Zhang Chuanlun Zhang A Novel Approach to Characterize the Lipidome of Marine Archaeon Nitrosopumilus maritimus by Ion Mobility Mass Spectrometry
description	Archaea are differentiated from the other two domains of life by their biomolecular characteristics. One such characteristic is the unique structure and composition of their lipids. Characterization of the whole set of lipids in a biological system (the lipidome) remains technologically challenging. This is because the lipidome is innately complex, and not all lipid species are extractable, separable, or ionizable by a single analytical method. Furthermore, lipids are structurally and chemically diverse. Many lipids are isobaric or isomeric and often indistinguishable by the measurement of mass or even their fragmentation spectra. Here we developed a novel analytical protocol based on liquid chromatography ion mobility mass spectrometry to enhance the coverage of the lipidome and characterize the conformations of archaeal lipids by their collision cross-sections (CCSs). The measurements of ion mobility revealed the gas-phase ion chemistry of representative archaeal lipids and provided further insights into their attributions to the adaptability of archaea to environmental stresses. A comprehensive characterization of the lipidome of mesophilic marine thaumarchaeon, Nitrosopumilus maritimus (strain SCM1) revealed potentially an unreported phosphate- and sulfate-containing lipid candidate by negative ionization analysis. It was the first time that experimentally derived CCS values of archaeal lipids were reported. Discrimination of crenarchaeol and its proposed stereoisomer was, however, not achieved with the resolving power of the SYNAPT G2 ion mobility system, and a high-resolution ion mobility system may be required for future work. Structural and spectral libraries of archaeal lipids were constructed in non-vendor-specific formats and are being made available to the community to promote research of Archaea by lipidomics.
format	article
author	Kai P. Law Kai P. Law Kai P. Law Wei He Wei He Jianchang Tao Jianchang Tao Chuanlun Zhang Chuanlun Zhang Chuanlun Zhang
author_facet	Kai P. Law Kai P. Law Kai P. Law Wei He Wei He Jianchang Tao Jianchang Tao Chuanlun Zhang Chuanlun Zhang Chuanlun Zhang
author_sort	Kai P. Law
title	A Novel Approach to Characterize the Lipidome of Marine Archaeon Nitrosopumilus maritimus by Ion Mobility Mass Spectrometry
title_short	A Novel Approach to Characterize the Lipidome of Marine Archaeon Nitrosopumilus maritimus by Ion Mobility Mass Spectrometry
title_full	A Novel Approach to Characterize the Lipidome of Marine Archaeon Nitrosopumilus maritimus by Ion Mobility Mass Spectrometry
title_fullStr	A Novel Approach to Characterize the Lipidome of Marine Archaeon Nitrosopumilus maritimus by Ion Mobility Mass Spectrometry
title_full_unstemmed	A Novel Approach to Characterize the Lipidome of Marine Archaeon Nitrosopumilus maritimus by Ion Mobility Mass Spectrometry
title_sort	novel approach to characterize the lipidome of marine archaeon nitrosopumilus maritimus by ion mobility mass spectrometry
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/abb440dde33c46b38a715b213d247934
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A Novel Approach to Characterize the Lipidome of Marine Archaeon Nitrosopumilus maritimus by Ion Mobility Mass Spectrometry

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