Phenotypic and molecular characterization of extended-spectrum beta-lactamase-producing Escherichia coli in Bangladesh.
<h4>Background</h4>Resistance to cephalosporins in Enterobacteriaceae is mainly due to the production of extended-spectrum beta-lactamase (ESBL). Little is known about ESBL-producing bacteria in Bangladesh. Therefore, the study presents results of phenotypic and molecular characterizatio...
Guardado en:
| Autores principales: | , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2014
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/abbf45f7ac1b47f3bba8aff56e480cc2 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:abbf45f7ac1b47f3bba8aff56e480cc2 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:abbf45f7ac1b47f3bba8aff56e480cc22021-11-25T05:57:12ZPhenotypic and molecular characterization of extended-spectrum beta-lactamase-producing Escherichia coli in Bangladesh.1932-620310.1371/journal.pone.0108735https://doaj.org/article/abbf45f7ac1b47f3bba8aff56e480cc22014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0108735https://doaj.org/toc/1932-6203<h4>Background</h4>Resistance to cephalosporins in Enterobacteriaceae is mainly due to the production of extended-spectrum beta-lactamase (ESBL). Little is known about ESBL-producing bacteria in Bangladesh. Therefore, the study presents results of phenotypic and molecular characterization of ESBL-producing Escherichia coli from hospitals in Bangladesh.<h4>Methods</h4>A total of 339 E. coli isolated from patients with urinary tract and wound infections attending three different medical hospitals in urban and rural areas of Bangladesh between 2003-2007 were screened for ESBL-production by the double disk diffusion test. Isolates with ESBL-phenotype were further characterized by antibiotic susceptibility testing, PCR and sequencing of different β-lactamase and virulence genes, serotyping, and XbaI-macrorestriction followed by pulsed-field gel electrophoresis (PFGE).<h4>Results</h4>We identified 40 E. coli with ESBL phenotype. These isolates were resistant to ceftriaxone, ceftazidime, cefotaxime, aztreonam, cefepime, and nalidixic acid but remained susceptible to imipenem. All but one isolate were additionally resistant to ciprofloxacin, and 3 isolates were resistant to cefoxitin. ESBL genes of blaCTX-M-1-group were detected in all isolates; blaTEM-type and blaOXA-1-type genes were detected in 33 (82.5%) and 19 (47.5%) isolates, respectively. Virulence genes that are present in diarrhoeagenic E. coli were not found. Class-1 integron was present in 20 (50%) isolates. All the ESBL-producing E. coli isolates harbored plasmids ranging between 1.1 and 120 MDa. PFGE-typing revealed 26 different pulsotypes, but identical pulsotype showed 6 isolates of serotype O25:H4.<h4>Conclusion</h4>The prevalence of multidrug-resistant ESBL-producing E. coli isolates appears to be high and the majority of the isolates were positive for blaCTX-M. Although there was genetic heterogeneity among isolates, presence of a cluster of isolates belonging to serotype O25:H4 indicates dissemination of the pandemic uropathogenic E. coli clone in Bangladesh.Taslima T LinaBijay K KhajanchiIshrat J AzmiMohammad Aminul IslamBelal MahmoodMahmuda AkterAtanu BanikRumana AlimArmando NavarroGabriel PerezAlejandro CraviotoKaisar A TalukderPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e108735 (2014) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Taslima T Lina Bijay K Khajanchi Ishrat J Azmi Mohammad Aminul Islam Belal Mahmood Mahmuda Akter Atanu Banik Rumana Alim Armando Navarro Gabriel Perez Alejandro Cravioto Kaisar A Talukder Phenotypic and molecular characterization of extended-spectrum beta-lactamase-producing Escherichia coli in Bangladesh. |
| description |
<h4>Background</h4>Resistance to cephalosporins in Enterobacteriaceae is mainly due to the production of extended-spectrum beta-lactamase (ESBL). Little is known about ESBL-producing bacteria in Bangladesh. Therefore, the study presents results of phenotypic and molecular characterization of ESBL-producing Escherichia coli from hospitals in Bangladesh.<h4>Methods</h4>A total of 339 E. coli isolated from patients with urinary tract and wound infections attending three different medical hospitals in urban and rural areas of Bangladesh between 2003-2007 were screened for ESBL-production by the double disk diffusion test. Isolates with ESBL-phenotype were further characterized by antibiotic susceptibility testing, PCR and sequencing of different β-lactamase and virulence genes, serotyping, and XbaI-macrorestriction followed by pulsed-field gel electrophoresis (PFGE).<h4>Results</h4>We identified 40 E. coli with ESBL phenotype. These isolates were resistant to ceftriaxone, ceftazidime, cefotaxime, aztreonam, cefepime, and nalidixic acid but remained susceptible to imipenem. All but one isolate were additionally resistant to ciprofloxacin, and 3 isolates were resistant to cefoxitin. ESBL genes of blaCTX-M-1-group were detected in all isolates; blaTEM-type and blaOXA-1-type genes were detected in 33 (82.5%) and 19 (47.5%) isolates, respectively. Virulence genes that are present in diarrhoeagenic E. coli were not found. Class-1 integron was present in 20 (50%) isolates. All the ESBL-producing E. coli isolates harbored plasmids ranging between 1.1 and 120 MDa. PFGE-typing revealed 26 different pulsotypes, but identical pulsotype showed 6 isolates of serotype O25:H4.<h4>Conclusion</h4>The prevalence of multidrug-resistant ESBL-producing E. coli isolates appears to be high and the majority of the isolates were positive for blaCTX-M. Although there was genetic heterogeneity among isolates, presence of a cluster of isolates belonging to serotype O25:H4 indicates dissemination of the pandemic uropathogenic E. coli clone in Bangladesh. |
| format |
article |
| author |
Taslima T Lina Bijay K Khajanchi Ishrat J Azmi Mohammad Aminul Islam Belal Mahmood Mahmuda Akter Atanu Banik Rumana Alim Armando Navarro Gabriel Perez Alejandro Cravioto Kaisar A Talukder |
| author_facet |
Taslima T Lina Bijay K Khajanchi Ishrat J Azmi Mohammad Aminul Islam Belal Mahmood Mahmuda Akter Atanu Banik Rumana Alim Armando Navarro Gabriel Perez Alejandro Cravioto Kaisar A Talukder |
| author_sort |
Taslima T Lina |
| title |
Phenotypic and molecular characterization of extended-spectrum beta-lactamase-producing Escherichia coli in Bangladesh. |
| title_short |
Phenotypic and molecular characterization of extended-spectrum beta-lactamase-producing Escherichia coli in Bangladesh. |
| title_full |
Phenotypic and molecular characterization of extended-spectrum beta-lactamase-producing Escherichia coli in Bangladesh. |
| title_fullStr |
Phenotypic and molecular characterization of extended-spectrum beta-lactamase-producing Escherichia coli in Bangladesh. |
| title_full_unstemmed |
Phenotypic and molecular characterization of extended-spectrum beta-lactamase-producing Escherichia coli in Bangladesh. |
| title_sort |
phenotypic and molecular characterization of extended-spectrum beta-lactamase-producing escherichia coli in bangladesh. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/abbf45f7ac1b47f3bba8aff56e480cc2 |
| work_keys_str_mv |
AT taslimatlina phenotypicandmolecularcharacterizationofextendedspectrumbetalactamaseproducingescherichiacoliinbangladesh AT bijaykkhajanchi phenotypicandmolecularcharacterizationofextendedspectrumbetalactamaseproducingescherichiacoliinbangladesh AT ishratjazmi phenotypicandmolecularcharacterizationofextendedspectrumbetalactamaseproducingescherichiacoliinbangladesh AT mohammadaminulislam phenotypicandmolecularcharacterizationofextendedspectrumbetalactamaseproducingescherichiacoliinbangladesh AT belalmahmood phenotypicandmolecularcharacterizationofextendedspectrumbetalactamaseproducingescherichiacoliinbangladesh AT mahmudaakter phenotypicandmolecularcharacterizationofextendedspectrumbetalactamaseproducingescherichiacoliinbangladesh AT atanubanik phenotypicandmolecularcharacterizationofextendedspectrumbetalactamaseproducingescherichiacoliinbangladesh AT rumanaalim phenotypicandmolecularcharacterizationofextendedspectrumbetalactamaseproducingescherichiacoliinbangladesh AT armandonavarro phenotypicandmolecularcharacterizationofextendedspectrumbetalactamaseproducingescherichiacoliinbangladesh AT gabrielperez phenotypicandmolecularcharacterizationofextendedspectrumbetalactamaseproducingescherichiacoliinbangladesh AT alejandrocravioto phenotypicandmolecularcharacterizationofextendedspectrumbetalactamaseproducingescherichiacoliinbangladesh AT kaisaratalukder phenotypicandmolecularcharacterizationofextendedspectrumbetalactamaseproducingescherichiacoliinbangladesh |
| _version_ |
1718414359540006912 |