YgfY Contributes to Stress Tolerance in <i>Shewanella oneidensis</i> Neither as an Antitoxin Nor as a Flavinylation Factor of Succinate Dehydrogenase

YgfY(SdhE/CptB) is highly conserved while has controversial functions in bacteria. It works as an antitoxin and composes a type IV toxin–antitoxin system with YgfX(CptA) typically in <i>Escherichia coli</i>, while functions as an flavinylation factor of succinate dehydrogenase and fumara...

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Autores principales:	Ming-Xing Zhang, Kai-Li Zheng, Ai-Guo Tang, Xiao-Xia Hu, Xin-Xin Guo, Chao Wu, Yuan-Yuan Cheng
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	YgfY <i>Shewanella</i> toxin–antitoxin succinate dehydrogenase flavinylation Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/abc70ead58b84d909a3d7599f8350147
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spelling	oai:doaj.org-article:abc70ead58b84d909a3d7599f83501472021-11-25T18:25:04ZYgfY Contributes to Stress Tolerance in <i>Shewanella oneidensis</i> Neither as an Antitoxin Nor as a Flavinylation Factor of Succinate Dehydrogenase10.3390/microorganisms91123162076-2607https://doaj.org/article/abc70ead58b84d909a3d7599f83501472021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2316https://doaj.org/toc/2076-2607YgfY(SdhE/CptB) is highly conserved while has controversial functions in bacteria. It works as an antitoxin and composes a type IV toxin–antitoxin system with YgfX(CptA) typically in <i>Escherichia coli</i>, while functions as an flavinylation factor of succinate dehydrogenase and fumarate reductase typically in <i>Serratia</i> sp. In this study, we report the contribution of YgfY in <i>Shewanella oneidensis</i> MR-1 to tolerance of low temperature and nitrite. YgfY deficiency causes several growth defects of <i>S. oneidensis</i> MR-1 at low temperature, while YgfX do not cause a growth defect or morphological change of <i>S. oneidensis</i> MR1-1 and <i>E. coli</i>. YgfY do not interact with FtsZ and MreB nor with YgfX examined by bacterial two-hybrid assay. YgfY effect on growth under low temperature is not attributed to succinate dehydrogenase (SDH) because a mutant without SDH grows comparably with the wild-type strain in the presence of succinate. The <i>ygfY</i> mutant shows impaired tolerance to nitrite. Transcription of nitrite reductase and most ribosome proteins is significantly decreased in the <i>ygfY</i> mutant, which is consistent with the phenotypes detected above. Effects of YgfY on growth and nitrite tolerance are closely related to the RGXXE motif in YgfY. In summary, this study demonstrates pleiotropic impacts of YgfY in <i>S. oneidensis</i> MR-1, and sheds a light on the physiological versatility of YgfY in bacteria.Ming-Xing ZhangKai-Li ZhengAi-Guo TangXiao-Xia HuXin-Xin GuoChao WuYuan-Yuan ChengMDPI AGarticleYgfY<i>Shewanella</i>toxin–antitoxinsuccinate dehydrogenaseflavinylationBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2316, p 2316 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	YgfY <i>Shewanella</i> toxin–antitoxin succinate dehydrogenase flavinylation Biology (General) QH301-705.5
spellingShingle	YgfY <i>Shewanella</i> toxin–antitoxin succinate dehydrogenase flavinylation Biology (General) QH301-705.5 Ming-Xing Zhang Kai-Li Zheng Ai-Guo Tang Xiao-Xia Hu Xin-Xin Guo Chao Wu Yuan-Yuan Cheng YgfY Contributes to Stress Tolerance in <i>Shewanella oneidensis</i> Neither as an Antitoxin Nor as a Flavinylation Factor of Succinate Dehydrogenase
description	YgfY(SdhE/CptB) is highly conserved while has controversial functions in bacteria. It works as an antitoxin and composes a type IV toxin–antitoxin system with YgfX(CptA) typically in <i>Escherichia coli</i>, while functions as an flavinylation factor of succinate dehydrogenase and fumarate reductase typically in <i>Serratia</i> sp. In this study, we report the contribution of YgfY in <i>Shewanella oneidensis</i> MR-1 to tolerance of low temperature and nitrite. YgfY deficiency causes several growth defects of <i>S. oneidensis</i> MR-1 at low temperature, while YgfX do not cause a growth defect or morphological change of <i>S. oneidensis</i> MR1-1 and <i>E. coli</i>. YgfY do not interact with FtsZ and MreB nor with YgfX examined by bacterial two-hybrid assay. YgfY effect on growth under low temperature is not attributed to succinate dehydrogenase (SDH) because a mutant without SDH grows comparably with the wild-type strain in the presence of succinate. The <i>ygfY</i> mutant shows impaired tolerance to nitrite. Transcription of nitrite reductase and most ribosome proteins is significantly decreased in the <i>ygfY</i> mutant, which is consistent with the phenotypes detected above. Effects of YgfY on growth and nitrite tolerance are closely related to the RGXXE motif in YgfY. In summary, this study demonstrates pleiotropic impacts of YgfY in <i>S. oneidensis</i> MR-1, and sheds a light on the physiological versatility of YgfY in bacteria.
format	article
author	Ming-Xing Zhang Kai-Li Zheng Ai-Guo Tang Xiao-Xia Hu Xin-Xin Guo Chao Wu Yuan-Yuan Cheng
author_facet	Ming-Xing Zhang Kai-Li Zheng Ai-Guo Tang Xiao-Xia Hu Xin-Xin Guo Chao Wu Yuan-Yuan Cheng
author_sort	Ming-Xing Zhang
title	YgfY Contributes to Stress Tolerance in <i>Shewanella oneidensis</i> Neither as an Antitoxin Nor as a Flavinylation Factor of Succinate Dehydrogenase
title_short	YgfY Contributes to Stress Tolerance in <i>Shewanella oneidensis</i> Neither as an Antitoxin Nor as a Flavinylation Factor of Succinate Dehydrogenase
title_full	YgfY Contributes to Stress Tolerance in <i>Shewanella oneidensis</i> Neither as an Antitoxin Nor as a Flavinylation Factor of Succinate Dehydrogenase
title_fullStr	YgfY Contributes to Stress Tolerance in <i>Shewanella oneidensis</i> Neither as an Antitoxin Nor as a Flavinylation Factor of Succinate Dehydrogenase
title_full_unstemmed	YgfY Contributes to Stress Tolerance in <i>Shewanella oneidensis</i> Neither as an Antitoxin Nor as a Flavinylation Factor of Succinate Dehydrogenase
title_sort	ygfy contributes to stress tolerance in <i>shewanella oneidensis</i> neither as an antitoxin nor as a flavinylation factor of succinate dehydrogenase
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/abc70ead58b84d909a3d7599f8350147
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YgfY Contributes to Stress Tolerance in <i>Shewanella oneidensis</i> Neither as an Antitoxin Nor as a Flavinylation Factor of Succinate Dehydrogenase

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