Identification of pathogenic genes in Campylobacter jejuni isolated from broiler carcasses and broiler slaughterhouses

Abstract Campylobacter jejuni is one of the most common causes of foodborne diseases worldwide. There are few reports on Campylobacter strains isolated from Latin-American countries. Here, 140 C. jejuni strains isolated from cloacal and transport boxes swabs, water from chiller tanks, and broiler ca...

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Autores principales:	Yuli Melisa Sierra-Arguello, Gustavo Perdoncini, Laura Beatriz Rodrigues, Luciana Ruschel dos Santos, Karen Apellanis Borges, Thales Quedi Furian, Carlos Tadeu Pippi Salle, Hamilton Luiz de Souza Moraes, Marcos José Pereira Gomes, Vladimir Pinheiro do Nascimento
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/abc966b082a445cb9307777f9d09c926
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spelling	oai:doaj.org-article:abc966b082a445cb9307777f9d09c9262021-12-02T13:34:52ZIdentification of pathogenic genes in Campylobacter jejuni isolated from broiler carcasses and broiler slaughterhouses10.1038/s41598-021-84149-12045-2322https://doaj.org/article/abc966b082a445cb9307777f9d09c9262021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84149-1https://doaj.org/toc/2045-2322Abstract Campylobacter jejuni is one of the most common causes of foodborne diseases worldwide. There are few reports on Campylobacter strains isolated from Latin-American countries. Here, 140 C. jejuni strains isolated from cloacal and transport boxes swabs, water from chiller tanks, and broiler carcasses of five poultry companies in Southern Brazil were identified using phenotypic and genotypic methods. Polymerase chain reaction (PCR) was used to analyze eight C. jejuni virulence markers: flaA, cadF, and invasion-associated (iam) genes, cdtABC operon (associated with the cytolethal distending toxin), and plasmidial virB11 and wlaN genes were present in 78.5%, 77.8%, 0%, 74.2%, 22.1%, and 10.7% of samples, respectively. There were 25 different virulence profiles: 1 (cdtA, cdtB, cdtC, flaA, and cadF), 2 (cdtA, cdtB, cdtC, flaA, cadF, and virB11), and 3 (cdtA, cdtB, cdtC, flaA, cadF, and wlaN) were the most common (> 60% of strains). We provide insight into factors related to the occurrence of this pathogen and their epidemiology.Yuli Melisa Sierra-ArguelloGustavo PerdonciniLaura Beatriz RodriguesLuciana Ruschel dos SantosKaren Apellanis BorgesThales Quedi FurianCarlos Tadeu Pippi SalleHamilton Luiz de Souza MoraesMarcos José Pereira GomesVladimir Pinheiro do NascimentoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuli Melisa Sierra-Arguello Gustavo Perdoncini Laura Beatriz Rodrigues Luciana Ruschel dos Santos Karen Apellanis Borges Thales Quedi Furian Carlos Tadeu Pippi Salle Hamilton Luiz de Souza Moraes Marcos José Pereira Gomes Vladimir Pinheiro do Nascimento Identification of pathogenic genes in Campylobacter jejuni isolated from broiler carcasses and broiler slaughterhouses
description	Abstract Campylobacter jejuni is one of the most common causes of foodborne diseases worldwide. There are few reports on Campylobacter strains isolated from Latin-American countries. Here, 140 C. jejuni strains isolated from cloacal and transport boxes swabs, water from chiller tanks, and broiler carcasses of five poultry companies in Southern Brazil were identified using phenotypic and genotypic methods. Polymerase chain reaction (PCR) was used to analyze eight C. jejuni virulence markers: flaA, cadF, and invasion-associated (iam) genes, cdtABC operon (associated with the cytolethal distending toxin), and plasmidial virB11 and wlaN genes were present in 78.5%, 77.8%, 0%, 74.2%, 22.1%, and 10.7% of samples, respectively. There were 25 different virulence profiles: 1 (cdtA, cdtB, cdtC, flaA, and cadF), 2 (cdtA, cdtB, cdtC, flaA, cadF, and virB11), and 3 (cdtA, cdtB, cdtC, flaA, cadF, and wlaN) were the most common (> 60% of strains). We provide insight into factors related to the occurrence of this pathogen and their epidemiology.
format	article
author	Yuli Melisa Sierra-Arguello Gustavo Perdoncini Laura Beatriz Rodrigues Luciana Ruschel dos Santos Karen Apellanis Borges Thales Quedi Furian Carlos Tadeu Pippi Salle Hamilton Luiz de Souza Moraes Marcos José Pereira Gomes Vladimir Pinheiro do Nascimento
author_facet	Yuli Melisa Sierra-Arguello Gustavo Perdoncini Laura Beatriz Rodrigues Luciana Ruschel dos Santos Karen Apellanis Borges Thales Quedi Furian Carlos Tadeu Pippi Salle Hamilton Luiz de Souza Moraes Marcos José Pereira Gomes Vladimir Pinheiro do Nascimento
author_sort	Yuli Melisa Sierra-Arguello
title	Identification of pathogenic genes in Campylobacter jejuni isolated from broiler carcasses and broiler slaughterhouses
title_short	Identification of pathogenic genes in Campylobacter jejuni isolated from broiler carcasses and broiler slaughterhouses
title_full	Identification of pathogenic genes in Campylobacter jejuni isolated from broiler carcasses and broiler slaughterhouses
title_fullStr	Identification of pathogenic genes in Campylobacter jejuni isolated from broiler carcasses and broiler slaughterhouses
title_full_unstemmed	Identification of pathogenic genes in Campylobacter jejuni isolated from broiler carcasses and broiler slaughterhouses
title_sort	identification of pathogenic genes in campylobacter jejuni isolated from broiler carcasses and broiler slaughterhouses
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/abc966b082a445cb9307777f9d09c926
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Identification of pathogenic genes in Campylobacter jejuni isolated from broiler carcasses and broiler slaughterhouses

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