High-affinity carboxyl-graphene oxide-based SPR aptasensor for the detection of hCG protein in clinical serum samples

Nan-Fu Chiu,1 Chia-Tzu Kuo,1 Chen-Yu Chen2,31Laboratory of Nano-Photonics and Biosensors, Institute of Electro-Optical Engineering, National Taiwan Normal University, Taipei 11677, Taiwan; 2Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei City 10449, Taiwan; 3Department of M...

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Autores principales:	Chiu NF, Kuo CT, Chen CY
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Carboxyl-graphene oxide (carboxyl-GO) surface plasmon resonance (SPR) human chorionic gonadotropin (hCG). Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/abc9973b480d4fa1bf54cd353460e3be
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spelling	oai:doaj.org-article:abc9973b480d4fa1bf54cd353460e3be2021-12-02T03:06:23ZHigh-affinity carboxyl-graphene oxide-based SPR aptasensor for the detection of hCG protein in clinical serum samples1178-2013https://doaj.org/article/abc9973b480d4fa1bf54cd353460e3be2019-07-01T00:00:00Zhttps://www.dovepress.com/high-affinity-carboxyl-graphene-oxide-based-spr-aptasensor-for-the-det-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Nan-Fu Chiu,1 Chia-Tzu Kuo,1 Chen-Yu Chen2,31Laboratory of Nano-Photonics and Biosensors, Institute of Electro-Optical Engineering, National Taiwan Normal University, Taipei 11677, Taiwan; 2Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei City 10449, Taiwan; 3Department of Medicine, Mackay Medical College, Taipei 252, TaiwanBackground: The use of functionalized graphene oxide (fGO) has led to a new trend in the sensor field, owing to its high sensitivity with regards to sensing characteristics and easy synthesis procedures.Methods: In this study, we developed an ultra-sensitive carboxyl-graphene oxide (carboxyl-GO)-based surface plasmon resonance (SPR) aptasensor using peptides to detect human chorionic gonadotropin (hCG) in clinical serum samples. The carboxyl-GO based SPR aptasensor provided high affinity and stronger binding of peptides, which are great importance to allow for a non-immunological label-free mechanism. Also, it allows the detection of low concentrations of hCG, which are in turn considered to be important clinical parameters to diagnose ectopic pregnancies and paraneoplastic syndromes.Results: The high selectivity of the carboxyl-GO-based SPR aptasensor for hCG recombinant protein was verified by the addition of the interfering proteins bovine serum albumin (BSA) and human serum albumin (HSA), which did not affect the sensitivity of the sensor. The carboxyl-GO-based chip can enhance the assay efficacy of interactions between peptides and had a high affinity binding for a ka of 17×106 M−1S−1. The limit of detection for hCG in clinical serum samples was 1.15 pg/mLConclusion: The results of this study demonstrated that the carboxyl-GO-based SPR aptasensor had excellent sensitivity, affinity and selectivity, and thus the potential to be used as disease-related biomarker assay to allow for an early diagnosis, and possibly a new area in the field of biochemical sensing technology.Keywords: carboxyl-graphene oxide (carboxyl-GO), surface plasmon resonance (SPR), human chorionic gonadotropin (hCG)Chiu NFKuo CTChen CYDove Medical PressarticleCarboxyl-graphene oxide (carboxyl-GO)surface plasmon resonance (SPR)human chorionic gonadotropin (hCG).Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 4833-4847 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Carboxyl-graphene oxide (carboxyl-GO) surface plasmon resonance (SPR) human chorionic gonadotropin (hCG). Medicine (General) R5-920
spellingShingle	Carboxyl-graphene oxide (carboxyl-GO) surface plasmon resonance (SPR) human chorionic gonadotropin (hCG). Medicine (General) R5-920 Chiu NF Kuo CT Chen CY High-affinity carboxyl-graphene oxide-based SPR aptasensor for the detection of hCG protein in clinical serum samples
description	Nan-Fu Chiu,1 Chia-Tzu Kuo,1 Chen-Yu Chen2,31Laboratory of Nano-Photonics and Biosensors, Institute of Electro-Optical Engineering, National Taiwan Normal University, Taipei 11677, Taiwan; 2Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei City 10449, Taiwan; 3Department of Medicine, Mackay Medical College, Taipei 252, TaiwanBackground: The use of functionalized graphene oxide (fGO) has led to a new trend in the sensor field, owing to its high sensitivity with regards to sensing characteristics and easy synthesis procedures.Methods: In this study, we developed an ultra-sensitive carboxyl-graphene oxide (carboxyl-GO)-based surface plasmon resonance (SPR) aptasensor using peptides to detect human chorionic gonadotropin (hCG) in clinical serum samples. The carboxyl-GO based SPR aptasensor provided high affinity and stronger binding of peptides, which are great importance to allow for a non-immunological label-free mechanism. Also, it allows the detection of low concentrations of hCG, which are in turn considered to be important clinical parameters to diagnose ectopic pregnancies and paraneoplastic syndromes.Results: The high selectivity of the carboxyl-GO-based SPR aptasensor for hCG recombinant protein was verified by the addition of the interfering proteins bovine serum albumin (BSA) and human serum albumin (HSA), which did not affect the sensitivity of the sensor. The carboxyl-GO-based chip can enhance the assay efficacy of interactions between peptides and had a high affinity binding for a ka of 17×106 M−1S−1. The limit of detection for hCG in clinical serum samples was 1.15 pg/mLConclusion: The results of this study demonstrated that the carboxyl-GO-based SPR aptasensor had excellent sensitivity, affinity and selectivity, and thus the potential to be used as disease-related biomarker assay to allow for an early diagnosis, and possibly a new area in the field of biochemical sensing technology.Keywords: carboxyl-graphene oxide (carboxyl-GO), surface plasmon resonance (SPR), human chorionic gonadotropin (hCG)
format	article
author	Chiu NF Kuo CT Chen CY
author_facet	Chiu NF Kuo CT Chen CY
author_sort	Chiu NF
title	High-affinity carboxyl-graphene oxide-based SPR aptasensor for the detection of hCG protein in clinical serum samples
title_short	High-affinity carboxyl-graphene oxide-based SPR aptasensor for the detection of hCG protein in clinical serum samples
title_full	High-affinity carboxyl-graphene oxide-based SPR aptasensor for the detection of hCG protein in clinical serum samples
title_fullStr	High-affinity carboxyl-graphene oxide-based SPR aptasensor for the detection of hCG protein in clinical serum samples
title_full_unstemmed	High-affinity carboxyl-graphene oxide-based SPR aptasensor for the detection of hCG protein in clinical serum samples
title_sort	high-affinity carboxyl-graphene oxide-based spr aptasensor for the detection of hcg protein in clinical serum samples
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/abc9973b480d4fa1bf54cd353460e3be
work_keys_str_mv	AT chiunf highaffinitycarboxylgrapheneoxidebasedspraptasensorforthedetectionofhcgproteininclinicalserumsamples AT kuoct highaffinitycarboxylgrapheneoxidebasedspraptasensorforthedetectionofhcgproteininclinicalserumsamples AT chency highaffinitycarboxylgrapheneoxidebasedspraptasensorforthedetectionofhcgproteininclinicalserumsamples
_version_	1718401940238368768

High-affinity carboxyl-graphene oxide-based SPR aptasensor for the detection of hCG protein in clinical serum samples

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