Molecular and morphological characterization of EMS-induced chili pepper mutants resistant to Chili veinal mottle virus

Abstract. Manzila I, Priyatno TP, Nugroho K, Terryana RT, Lestari P, Hidayat SH. 2020. Molecular and morphological characterization of EMS-induced chili pepper mutants resistant to Chili veinal mottle virus. Biodiversitas 21: 1448-1457. Chili veinal mottle virus (ChiVMV) is a major prevalent virus t...

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Autores principales:	Ifa Manzila, Tri Puji Priyatno, KRISTIANTO NUGROHO, RERENSTRADIKA TIZAR TERRYANA, puji Lestari, SRI HENDRASTUTI HIDAYAT
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	capsicum annuum, chili veinal mottle virus, mutant lines, ssr Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/abc9a97e03494875a97cc9d001791a51
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spelling	oai:doaj.org-article:abc9a97e03494875a97cc9d001791a512021-11-22T00:34:40ZMolecular and morphological characterization of EMS-induced chili pepper mutants resistant to Chili veinal mottle virus1412-033X2085-472210.13057/biodiv/d210424https://doaj.org/article/abc9a97e03494875a97cc9d001791a512020-03-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4698https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Manzila I, Priyatno TP, Nugroho K, Terryana RT, Lestari P, Hidayat SH. 2020. Molecular and morphological characterization of EMS-induced chili pepper mutants resistant to Chili veinal mottle virus. Biodiversitas 21: 1448-1457. Chili veinal mottle virus (ChiVMV) is a major prevalent virus that potentially reduces the yield of chili pepper by more than 50% in Indonesia. Therefore, the development of a ChiVMV-resistant cultivar is necessary to ensure satisfactory chili pepper production. Mutation breeding is a promising tool available to produce virus-resistant plants via the induction of new alleles due to point mutations within the existing chili germplasm. This research was conducted to assess the genetic variation of ten promising (M6) EMS-induced mutant lines of chili pepper (Capsicum annuum L.) potentially resistant to ChiVMV by their morphological characteristics and SSR profile, as well as identify potential mutants based on agronomic traits. The ten chili pepper mutant lines used in the present study were derived from the shoot tips of a Gelora cultivar treated with 0.5% ethyl methanesulfonate (EMS) as a chemical mutagen. The performance of the respective promising chili pepper mutant lines both at a molecular and morphological level was observed. Phenotypic analysis showed that all the promising chili pepper mutant lines underwent significant morphological changes. Of these, the phenotype coefficient variation (PCV) values were higher than the genotype coefficient variation (GCV) values for all the traits observed. High heritability (h2) was found for the canopy area, length of the fruit stalk, fruit weight, fruit weight per plant, thick fruit flesh, leaf width, leaf length, and fruit storage duration. A total of 52 alleles were detected from the five polymorphic SSR loci with 5.2 average alleles/loci. A dendrogram was generated and revealed two groups with five mutants clustered separately from their parents which could be valuable for further improvement.Ifa ManzilaTri Puji PriyatnoKRISTIANTO NUGROHORERENSTRADIKA TIZAR TERRYANApuji LestariSRI HENDRASTUTI HIDAYATMBI & UNS Soloarticlecapsicum annuum, chili veinal mottle virus, mutant lines, ssrBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 4 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	capsicum annuum, chili veinal mottle virus, mutant lines, ssr Biology (General) QH301-705.5
spellingShingle	capsicum annuum, chili veinal mottle virus, mutant lines, ssr Biology (General) QH301-705.5 Ifa Manzila Tri Puji Priyatno KRISTIANTO NUGROHO RERENSTRADIKA TIZAR TERRYANA puji Lestari SRI HENDRASTUTI HIDAYAT Molecular and morphological characterization of EMS-induced chili pepper mutants resistant to Chili veinal mottle virus
description	Abstract. Manzila I, Priyatno TP, Nugroho K, Terryana RT, Lestari P, Hidayat SH. 2020. Molecular and morphological characterization of EMS-induced chili pepper mutants resistant to Chili veinal mottle virus. Biodiversitas 21: 1448-1457. Chili veinal mottle virus (ChiVMV) is a major prevalent virus that potentially reduces the yield of chili pepper by more than 50% in Indonesia. Therefore, the development of a ChiVMV-resistant cultivar is necessary to ensure satisfactory chili pepper production. Mutation breeding is a promising tool available to produce virus-resistant plants via the induction of new alleles due to point mutations within the existing chili germplasm. This research was conducted to assess the genetic variation of ten promising (M6) EMS-induced mutant lines of chili pepper (Capsicum annuum L.) potentially resistant to ChiVMV by their morphological characteristics and SSR profile, as well as identify potential mutants based on agronomic traits. The ten chili pepper mutant lines used in the present study were derived from the shoot tips of a Gelora cultivar treated with 0.5% ethyl methanesulfonate (EMS) as a chemical mutagen. The performance of the respective promising chili pepper mutant lines both at a molecular and morphological level was observed. Phenotypic analysis showed that all the promising chili pepper mutant lines underwent significant morphological changes. Of these, the phenotype coefficient variation (PCV) values were higher than the genotype coefficient variation (GCV) values for all the traits observed. High heritability (h2) was found for the canopy area, length of the fruit stalk, fruit weight, fruit weight per plant, thick fruit flesh, leaf width, leaf length, and fruit storage duration. A total of 52 alleles were detected from the five polymorphic SSR loci with 5.2 average alleles/loci. A dendrogram was generated and revealed two groups with five mutants clustered separately from their parents which could be valuable for further improvement.
format	article
author	Ifa Manzila Tri Puji Priyatno KRISTIANTO NUGROHO RERENSTRADIKA TIZAR TERRYANA puji Lestari SRI HENDRASTUTI HIDAYAT
author_facet	Ifa Manzila Tri Puji Priyatno KRISTIANTO NUGROHO RERENSTRADIKA TIZAR TERRYANA puji Lestari SRI HENDRASTUTI HIDAYAT
author_sort	Ifa Manzila
title	Molecular and morphological characterization of EMS-induced chili pepper mutants resistant to Chili veinal mottle virus
title_short	Molecular and morphological characterization of EMS-induced chili pepper mutants resistant to Chili veinal mottle virus
title_full	Molecular and morphological characterization of EMS-induced chili pepper mutants resistant to Chili veinal mottle virus
title_fullStr	Molecular and morphological characterization of EMS-induced chili pepper mutants resistant to Chili veinal mottle virus
title_full_unstemmed	Molecular and morphological characterization of EMS-induced chili pepper mutants resistant to Chili veinal mottle virus
title_sort	molecular and morphological characterization of ems-induced chili pepper mutants resistant to chili veinal mottle virus
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/abc9a97e03494875a97cc9d001791a51
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Molecular and morphological characterization of EMS-induced chili pepper mutants resistant to Chili veinal mottle virus

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