Reducing strength prevailing at root surface of plants promotes reduction of Ag+ and generation of Ag(0)/Ag2O nanoparticles exogenously in aqueous phase.

Potential of root system of plants from wide range of families to effectively reduce membrane impermeable ferricyanide to ferrocyanide and blue coloured 2,6-dichlorophenol indophenol (DCPIP) to colourless DCPIPH2 both under non-sterile and sterile conditions, revealed prevalence of immense reducing...

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Autores principales:	Peddisetty Pardha-Saradhi, Gupta Yamal, Tanuj Peddisetty, Peddisetty Sharmila, Shilpi Nagar, Jyoti Singh, Rajamani Nagarajan, Kottapalli S Rao
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/abe406729dbb4cd9aefc9579a79415b4
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spelling	oai:doaj.org-article:abe406729dbb4cd9aefc9579a79415b42021-11-25T06:02:09ZReducing strength prevailing at root surface of plants promotes reduction of Ag+ and generation of Ag(0)/Ag2O nanoparticles exogenously in aqueous phase.1932-620310.1371/journal.pone.0106715https://doaj.org/article/abe406729dbb4cd9aefc9579a79415b42014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25184239/?tool=EBIhttps://doaj.org/toc/1932-6203Potential of root system of plants from wide range of families to effectively reduce membrane impermeable ferricyanide to ferrocyanide and blue coloured 2,6-dichlorophenol indophenol (DCPIP) to colourless DCPIPH2 both under non-sterile and sterile conditions, revealed prevalence of immense reducing strength at root surface. As generation of silver nanoparticles (NPs) from Ag+ involves reduction, present investigations were carried to evaluate if reducing strength prevailing at surface of root system can be exploited for reduction of Ag+ and exogenous generation of silver-NPs. Root system of intact plants of 16 species from 11 diverse families of angiosperms turned clear colorless AgNO3 solutions, turbid brown. Absorption spectra of these turbid brown solutions showed silver-NPs specific surface plasmon resonance peak. Transmission electron microscope coupled with energy dispersive X-ray confirmed the presence of distinct NPs in the range of 5-50 nm containing Ag. Selected area electron diffraction and powder X-ray diffraction patterns of the silver NPs showed Bragg reflections, characteristic of crystalline face-centered cubic structure of Ag(0) and cubic structure of Ag2O. Root system of intact plants raised under sterile conditions also generated Ag(0)/Ag2O-NPs under strict sterile conditions in a manner similar to that recorded under non-sterile conditions. This revealed the inbuilt potential of root system to generate Ag(0)/Ag2O-NPs independent of any microorganism. Roots of intact plants reduced triphenyltetrazolium to triphenylformazon and impermeable ferricyanide to ferrocyanide, suggesting involvement of plasma membrane bound dehydrogenases in reduction of Ag+ and formation of Ag(0)/Ag2O-NPs. Root enzyme extract reduced triphenyltetrazolium to triphenylformazon and Ag+ to Ag(0) in presence of NADH, clearly establishing potential of dehydrogenases to reduce Ag+ to Ag(0), which generate Ag(0)/Ag2O-NPs. Findings presented in this manuscript put forth a novel, simple, economically viable and green protocol for synthesis of silver-NPs under ambient conditions in aqueous phase, using root system of intact plants.Peddisetty Pardha-SaradhiGupta YamalTanuj PeddisettyPeddisetty SharmilaShilpi NagarJyoti SinghRajamani NagarajanKottapalli S RaoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e106715 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Peddisetty Pardha-Saradhi Gupta Yamal Tanuj Peddisetty Peddisetty Sharmila Shilpi Nagar Jyoti Singh Rajamani Nagarajan Kottapalli S Rao Reducing strength prevailing at root surface of plants promotes reduction of Ag+ and generation of Ag(0)/Ag2O nanoparticles exogenously in aqueous phase.
description	Potential of root system of plants from wide range of families to effectively reduce membrane impermeable ferricyanide to ferrocyanide and blue coloured 2,6-dichlorophenol indophenol (DCPIP) to colourless DCPIPH2 both under non-sterile and sterile conditions, revealed prevalence of immense reducing strength at root surface. As generation of silver nanoparticles (NPs) from Ag+ involves reduction, present investigations were carried to evaluate if reducing strength prevailing at surface of root system can be exploited for reduction of Ag+ and exogenous generation of silver-NPs. Root system of intact plants of 16 species from 11 diverse families of angiosperms turned clear colorless AgNO3 solutions, turbid brown. Absorption spectra of these turbid brown solutions showed silver-NPs specific surface plasmon resonance peak. Transmission electron microscope coupled with energy dispersive X-ray confirmed the presence of distinct NPs in the range of 5-50 nm containing Ag. Selected area electron diffraction and powder X-ray diffraction patterns of the silver NPs showed Bragg reflections, characteristic of crystalline face-centered cubic structure of Ag(0) and cubic structure of Ag2O. Root system of intact plants raised under sterile conditions also generated Ag(0)/Ag2O-NPs under strict sterile conditions in a manner similar to that recorded under non-sterile conditions. This revealed the inbuilt potential of root system to generate Ag(0)/Ag2O-NPs independent of any microorganism. Roots of intact plants reduced triphenyltetrazolium to triphenylformazon and impermeable ferricyanide to ferrocyanide, suggesting involvement of plasma membrane bound dehydrogenases in reduction of Ag+ and formation of Ag(0)/Ag2O-NPs. Root enzyme extract reduced triphenyltetrazolium to triphenylformazon and Ag+ to Ag(0) in presence of NADH, clearly establishing potential of dehydrogenases to reduce Ag+ to Ag(0), which generate Ag(0)/Ag2O-NPs. Findings presented in this manuscript put forth a novel, simple, economically viable and green protocol for synthesis of silver-NPs under ambient conditions in aqueous phase, using root system of intact plants.
format	article
author	Peddisetty Pardha-Saradhi Gupta Yamal Tanuj Peddisetty Peddisetty Sharmila Shilpi Nagar Jyoti Singh Rajamani Nagarajan Kottapalli S Rao
author_facet	Peddisetty Pardha-Saradhi Gupta Yamal Tanuj Peddisetty Peddisetty Sharmila Shilpi Nagar Jyoti Singh Rajamani Nagarajan Kottapalli S Rao
author_sort	Peddisetty Pardha-Saradhi
title	Reducing strength prevailing at root surface of plants promotes reduction of Ag+ and generation of Ag(0)/Ag2O nanoparticles exogenously in aqueous phase.
title_short	Reducing strength prevailing at root surface of plants promotes reduction of Ag+ and generation of Ag(0)/Ag2O nanoparticles exogenously in aqueous phase.
title_full	Reducing strength prevailing at root surface of plants promotes reduction of Ag+ and generation of Ag(0)/Ag2O nanoparticles exogenously in aqueous phase.
title_fullStr	Reducing strength prevailing at root surface of plants promotes reduction of Ag+ and generation of Ag(0)/Ag2O nanoparticles exogenously in aqueous phase.
title_full_unstemmed	Reducing strength prevailing at root surface of plants promotes reduction of Ag+ and generation of Ag(0)/Ag2O nanoparticles exogenously in aqueous phase.
title_sort	reducing strength prevailing at root surface of plants promotes reduction of ag+ and generation of ag(0)/ag2o nanoparticles exogenously in aqueous phase.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/abe406729dbb4cd9aefc9579a79415b4
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Reducing strength prevailing at root surface of plants promotes reduction of Ag+ and generation of Ag(0)/Ag2O nanoparticles exogenously in aqueous phase.

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