DNA barcoding and the associated PhylAphidB@se website for the identification of European aphids (Insecta: Hemiptera: Aphididae).

<h4>Unlabelled</h4>Aphids constitute a diverse group of plant-feeding insects and are among the most important crop pests in temperate regions. Their morphological identification is time-consuming and requires specific knowledge, training and skills that may take years to acquire. We ass...

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Autores principales:	Armelle Coeur d'Acier, Astrid Cruaud, Emmanuelle Artige, Gwenaëlle Genson, Anne-Laure Clamens, Eric Pierre, Sylvie Hudaverdian, Jean-Christophe Simon, Emmanuelle Jousselin, Jean-Yves Rasplus
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/abe675f327e54fcdaf083eec12825598
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spelling	oai:doaj.org-article:abe675f327e54fcdaf083eec128255982021-11-18T08:17:11ZDNA barcoding and the associated PhylAphidB@se website for the identification of European aphids (Insecta: Hemiptera: Aphididae).1932-620310.1371/journal.pone.0097620https://doaj.org/article/abe675f327e54fcdaf083eec128255982014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24896814/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Unlabelled</h4>Aphids constitute a diverse group of plant-feeding insects and are among the most important crop pests in temperate regions. Their morphological identification is time-consuming and requires specific knowledge, training and skills that may take years to acquire. We assessed the advantages and limits of DNA barcoding with the standard COI barcode fragment for the identification of European aphids. We constructed a large reference dataset of barcodes from 1020 specimens belonging to 274 species and 87 genera sampled throughout Europe and set up a database-driven website allowing species identification from query sequences.<h4>Results</h4>In this unbiased sampling of the taxonomic diversity of European aphids, intraspecific divergence ranged from 0.0% to 3.9%, with a mean value of 0.29%, whereas mean congeneric divergence was 6.4%, ranging from 0.0% to 15%. Neighbor-joining analysis generated a tree in which most species clustered in distinct genetic units. Most of the species with undifferentiated or overlapping barcodes belonged to the genus Aphis or, to a lesser extent, the genera Brachycaudus, Dysaphis and Macrosiphum. The taxa involved were always morphologically similar or closely related and belonged to species groups known to present taxonomic difficulties.<h4>Conclusions</h4>These data confirm that COI barcoding is a useful identification tool for aphids. Barcode identification is straightforward and reliable for 80% of species, including some difficult to distinguish on the basis of morphological characters alone. Unsurprisingly, barcodes often failed to distinguish between species from groups for which classical taxonomy has also reached its limits, leading to endless revisions and discussions about species and subspecies definitions. In such cases, the development of an effective procedure for the accurate identification of aphid specimens continues to pose a difficult challenge.Armelle Coeur d'AcierAstrid CruaudEmmanuelle ArtigeGwenaëlle GensonAnne-Laure ClamensEric PierreSylvie HudaverdianJean-Christophe SimonEmmanuelle JousselinJean-Yves RasplusPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e97620 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Armelle Coeur d'Acier Astrid Cruaud Emmanuelle Artige Gwenaëlle Genson Anne-Laure Clamens Eric Pierre Sylvie Hudaverdian Jean-Christophe Simon Emmanuelle Jousselin Jean-Yves Rasplus DNA barcoding and the associated PhylAphidB@se website for the identification of European aphids (Insecta: Hemiptera: Aphididae).
description	<h4>Unlabelled</h4>Aphids constitute a diverse group of plant-feeding insects and are among the most important crop pests in temperate regions. Their morphological identification is time-consuming and requires specific knowledge, training and skills that may take years to acquire. We assessed the advantages and limits of DNA barcoding with the standard COI barcode fragment for the identification of European aphids. We constructed a large reference dataset of barcodes from 1020 specimens belonging to 274 species and 87 genera sampled throughout Europe and set up a database-driven website allowing species identification from query sequences.<h4>Results</h4>In this unbiased sampling of the taxonomic diversity of European aphids, intraspecific divergence ranged from 0.0% to 3.9%, with a mean value of 0.29%, whereas mean congeneric divergence was 6.4%, ranging from 0.0% to 15%. Neighbor-joining analysis generated a tree in which most species clustered in distinct genetic units. Most of the species with undifferentiated or overlapping barcodes belonged to the genus Aphis or, to a lesser extent, the genera Brachycaudus, Dysaphis and Macrosiphum. The taxa involved were always morphologically similar or closely related and belonged to species groups known to present taxonomic difficulties.<h4>Conclusions</h4>These data confirm that COI barcoding is a useful identification tool for aphids. Barcode identification is straightforward and reliable for 80% of species, including some difficult to distinguish on the basis of morphological characters alone. Unsurprisingly, barcodes often failed to distinguish between species from groups for which classical taxonomy has also reached its limits, leading to endless revisions and discussions about species and subspecies definitions. In such cases, the development of an effective procedure for the accurate identification of aphid specimens continues to pose a difficult challenge.
format	article
author	Armelle Coeur d'Acier Astrid Cruaud Emmanuelle Artige Gwenaëlle Genson Anne-Laure Clamens Eric Pierre Sylvie Hudaverdian Jean-Christophe Simon Emmanuelle Jousselin Jean-Yves Rasplus
author_facet	Armelle Coeur d'Acier Astrid Cruaud Emmanuelle Artige Gwenaëlle Genson Anne-Laure Clamens Eric Pierre Sylvie Hudaverdian Jean-Christophe Simon Emmanuelle Jousselin Jean-Yves Rasplus
author_sort	Armelle Coeur d'Acier
title	DNA barcoding and the associated PhylAphidB@se website for the identification of European aphids (Insecta: Hemiptera: Aphididae).
title_short	DNA barcoding and the associated PhylAphidB@se website for the identification of European aphids (Insecta: Hemiptera: Aphididae).
title_full	DNA barcoding and the associated PhylAphidB@se website for the identification of European aphids (Insecta: Hemiptera: Aphididae).
title_fullStr	DNA barcoding and the associated PhylAphidB@se website for the identification of European aphids (Insecta: Hemiptera: Aphididae).
title_full_unstemmed	DNA barcoding and the associated PhylAphidB@se website for the identification of European aphids (Insecta: Hemiptera: Aphididae).
title_sort	dna barcoding and the associated phylaphidb@se website for the identification of european aphids (insecta: hemiptera: aphididae).
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/abe675f327e54fcdaf083eec12825598
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DNA barcoding and the associated PhylAphidB@se website for the identification of European aphids (Insecta: Hemiptera: Aphididae).

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