β-Dispersion of blood during sedimentation

Abstract Aggregation of human red blood cells (RBC) is central to various pathological conditions from bacterial infections to cancer. When left at low shear conditions or at hemostasis, RBCs form aggregates, which resemble stacks of coins, known as ‘rouleaux’. We experimentally examined the interfa...

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Autores principales:	Ahmet C. Sabuncu, Sinan Muldur, Barbaros Cetin, O. Berk Usta, Nadine Aubry
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/abe7eee3f2a44f6d91232c9b0145a4cd
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id	oai:doaj.org-article:abe7eee3f2a44f6d91232c9b0145a4cd
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spelling	oai:doaj.org-article:abe7eee3f2a44f6d91232c9b0145a4cd2021-12-02T14:16:26Zβ-Dispersion of blood during sedimentation10.1038/s41598-021-82171-x2045-2322https://doaj.org/article/abe7eee3f2a44f6d91232c9b0145a4cd2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82171-xhttps://doaj.org/toc/2045-2322Abstract Aggregation of human red blood cells (RBC) is central to various pathological conditions from bacterial infections to cancer. When left at low shear conditions or at hemostasis, RBCs form aggregates, which resemble stacks of coins, known as ‘rouleaux’. We experimentally examined the interfacial dielectric dispersion of aggregating RBCs. Hetastarch, an RBC aggregation agent, is used to mimic conditions leading to aggregation. Hetastrach concentration is incrementally increased in blood from healthy donors to measure the sensitivity of the technique. Time lapse electrical impedance measurements were conducted as red blood cells form rouleaux and sediment in a PDMS chamber. Theoretical modeling was used for obtaining complex permittivity of an effective single red blood cell aggregate at various concentrations of hetastarch. Time response of red blood cells’ impedance was also studied to parametrize the time evolution of impedance data. Single aggregate permittivity at the onset of aggregation, evolution of interfacial dispersion parameters, and sedimentation kinetics allowed us to distinguish differential aggregation in blood.Ahmet C. SabuncuSinan MuldurBarbaros CetinO. Berk UstaNadine AubryNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ahmet C. Sabuncu Sinan Muldur Barbaros Cetin O. Berk Usta Nadine Aubry β-Dispersion of blood during sedimentation
description	Abstract Aggregation of human red blood cells (RBC) is central to various pathological conditions from bacterial infections to cancer. When left at low shear conditions or at hemostasis, RBCs form aggregates, which resemble stacks of coins, known as ‘rouleaux’. We experimentally examined the interfacial dielectric dispersion of aggregating RBCs. Hetastarch, an RBC aggregation agent, is used to mimic conditions leading to aggregation. Hetastrach concentration is incrementally increased in blood from healthy donors to measure the sensitivity of the technique. Time lapse electrical impedance measurements were conducted as red blood cells form rouleaux and sediment in a PDMS chamber. Theoretical modeling was used for obtaining complex permittivity of an effective single red blood cell aggregate at various concentrations of hetastarch. Time response of red blood cells’ impedance was also studied to parametrize the time evolution of impedance data. Single aggregate permittivity at the onset of aggregation, evolution of interfacial dispersion parameters, and sedimentation kinetics allowed us to distinguish differential aggregation in blood.
format	article
author	Ahmet C. Sabuncu Sinan Muldur Barbaros Cetin O. Berk Usta Nadine Aubry
author_facet	Ahmet C. Sabuncu Sinan Muldur Barbaros Cetin O. Berk Usta Nadine Aubry
author_sort	Ahmet C. Sabuncu
title	β-Dispersion of blood during sedimentation
title_short	β-Dispersion of blood during sedimentation
title_full	β-Dispersion of blood during sedimentation
title_fullStr	β-Dispersion of blood during sedimentation
title_full_unstemmed	β-Dispersion of blood during sedimentation
title_sort	β-dispersion of blood during sedimentation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/abe7eee3f2a44f6d91232c9b0145a4cd
work_keys_str_mv	AT ahmetcsabuncu bdispersionofbloodduringsedimentation AT sinanmuldur bdispersionofbloodduringsedimentation AT barbaroscetin bdispersionofbloodduringsedimentation AT oberkusta bdispersionofbloodduringsedimentation AT nadineaubry bdispersionofbloodduringsedimentation
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β-Dispersion of blood during sedimentation

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