NHF-derived carbon dots: prevalidation approach in breast cancer treatment

Abstract Metastatic breast cancer dominates the female cancer-related mortality. Tumour-associated molecules represents a crucial for early disease detection and identification of novel therapeutic targets. Nanomaterial technologies provide promising novel approaches to disease diagnostics and thera...

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Autores principales:	Crina Elena Tiron, Gabriel Luta, Mihail Butura, Florin Zugun-Eloae, Corneliu S. Stan, Adina Coroaba, Elena-Laura Ursu, Gabriela Dumitrita Stanciu, Adrian Tiron
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/abf2f4bf241c4ae490a603bd6ba5174c
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spelling	oai:doaj.org-article:abf2f4bf241c4ae490a603bd6ba5174c2021-12-02T16:06:41ZNHF-derived carbon dots: prevalidation approach in breast cancer treatment10.1038/s41598-020-69670-z2045-2322https://doaj.org/article/abf2f4bf241c4ae490a603bd6ba5174c2020-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-69670-zhttps://doaj.org/toc/2045-2322Abstract Metastatic breast cancer dominates the female cancer-related mortality. Tumour-associated molecules represents a crucial for early disease detection and identification of novel therapeutic targets. Nanomaterial technologies provide promising novel approaches to disease diagnostics and therapeutics. In the present study we extend the investigations of antitumoral properties of Carbon Dots prepared from N-hydroxyphthalimide (CD-NHF) precursor. We evaluate the effect of CD-NHF on tumour cell migration and invasion in vitro and their impact on tumour progression using an in vivo model. Furthermore, we investigate the molecular mechanisms involved in CD-NHF antitumour effects. In vivo mammary tumours were induced in Balb/c female mice by injecting 4T1 cells into the mammary fat pad. Conditional treatment with CD-NHF significantly impair both migration and invasion of metastatic breast cancer cells. The presence of CD-NHF within the 3D cell cultures strongly inhibited the malignant phenotype of MDA-MB-231, 4T1 and MCF-7 cells in 3D culture, resulting in culture colonies lacking invasive projections and reduction of mammospheres formation. Importantly, breast tumour growth and metastasis dissemination was significantly reduced upon CD-NHF treatments in a syngeneic mouse model and is associated with down-regulation of Ki67 and HSP90 expression. CD-NHF nanostructures provide exciting perspective for improving treatment outcome in breast cancer.Crina Elena TironGabriel LutaMihail ButuraFlorin Zugun-EloaeCorneliu S. StanAdina CoroabaElena-Laura UrsuGabriela Dumitrita StanciuAdrian TironNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Crina Elena Tiron Gabriel Luta Mihail Butura Florin Zugun-Eloae Corneliu S. Stan Adina Coroaba Elena-Laura Ursu Gabriela Dumitrita Stanciu Adrian Tiron NHF-derived carbon dots: prevalidation approach in breast cancer treatment
description	Abstract Metastatic breast cancer dominates the female cancer-related mortality. Tumour-associated molecules represents a crucial for early disease detection and identification of novel therapeutic targets. Nanomaterial technologies provide promising novel approaches to disease diagnostics and therapeutics. In the present study we extend the investigations of antitumoral properties of Carbon Dots prepared from N-hydroxyphthalimide (CD-NHF) precursor. We evaluate the effect of CD-NHF on tumour cell migration and invasion in vitro and their impact on tumour progression using an in vivo model. Furthermore, we investigate the molecular mechanisms involved in CD-NHF antitumour effects. In vivo mammary tumours were induced in Balb/c female mice by injecting 4T1 cells into the mammary fat pad. Conditional treatment with CD-NHF significantly impair both migration and invasion of metastatic breast cancer cells. The presence of CD-NHF within the 3D cell cultures strongly inhibited the malignant phenotype of MDA-MB-231, 4T1 and MCF-7 cells in 3D culture, resulting in culture colonies lacking invasive projections and reduction of mammospheres formation. Importantly, breast tumour growth and metastasis dissemination was significantly reduced upon CD-NHF treatments in a syngeneic mouse model and is associated with down-regulation of Ki67 and HSP90 expression. CD-NHF nanostructures provide exciting perspective for improving treatment outcome in breast cancer.
format	article
author	Crina Elena Tiron Gabriel Luta Mihail Butura Florin Zugun-Eloae Corneliu S. Stan Adina Coroaba Elena-Laura Ursu Gabriela Dumitrita Stanciu Adrian Tiron
author_facet	Crina Elena Tiron Gabriel Luta Mihail Butura Florin Zugun-Eloae Corneliu S. Stan Adina Coroaba Elena-Laura Ursu Gabriela Dumitrita Stanciu Adrian Tiron
author_sort	Crina Elena Tiron
title	NHF-derived carbon dots: prevalidation approach in breast cancer treatment
title_short	NHF-derived carbon dots: prevalidation approach in breast cancer treatment
title_full	NHF-derived carbon dots: prevalidation approach in breast cancer treatment
title_fullStr	NHF-derived carbon dots: prevalidation approach in breast cancer treatment
title_full_unstemmed	NHF-derived carbon dots: prevalidation approach in breast cancer treatment
title_sort	nhf-derived carbon dots: prevalidation approach in breast cancer treatment
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/abf2f4bf241c4ae490a603bd6ba5174c
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NHF-derived carbon dots: prevalidation approach in breast cancer treatment

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