Analysis of a gene family for PDF-like peptides from Arabidopsis

Abstract Plant defensins are small, basic peptides that have a characteristic three-dimensional folding pattern which is stabilized by four disulfide bridges. We show here that Arabidopsis contains in addition to the proper plant defensins a group of 9 plant defensin-like (PdfL) genes. They are all...

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Autores principales:	Reza Omidvar, Nadine Vosseler, Amjad Abbas, Birgit Gutmann, Clemens Grünwald-Gruber, Friedrich Altmann, Shahid Siddique, Holger Bohlmann
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/abff19f0cd8d459284a8c7d3c76f6ad2
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spelling	oai:doaj.org-article:abff19f0cd8d459284a8c7d3c76f6ad22021-12-02T18:48:02ZAnalysis of a gene family for PDF-like peptides from Arabidopsis10.1038/s41598-021-98175-62045-2322https://doaj.org/article/abff19f0cd8d459284a8c7d3c76f6ad22021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98175-6https://doaj.org/toc/2045-2322Abstract Plant defensins are small, basic peptides that have a characteristic three-dimensional folding pattern which is stabilized by four disulfide bridges. We show here that Arabidopsis contains in addition to the proper plant defensins a group of 9 plant defensin-like (PdfL) genes. They are all expressed at low levels while GUS fusions of the promoters showed expression in most tissues with only minor differences. We produced two of the encoded peptides in E. coli and tested the antimicrobial activity in vitro. Both were highly active against fungi but had lower activity against bacteria. At higher concentrations hyperbranching and swollen tips, which are indicative of antimicrobial activity, were induced in Fusarium graminearum by both peptides. Overexpression lines for most PdfL genes were produced using the 35S CaMV promoter to study their possible in planta function. With the exception of PdfL4.1 these lines had enhanced resistance against F. oxysporum. All PDFL peptides were also transiently expressed in Nicotiana benthamiana leaves with agroinfiltration using the pPZP3425 vector. In case of PDFL1.4 this resulted in complete death of the infiltrated tissues after 7 days. All other PDFLs resulted only in various degrees of small necrotic lesions. In conclusion, our results show that at least some of the PdfL genes could function in plant resistance.Reza OmidvarNadine VosselerAmjad AbbasBirgit GutmannClemens Grünwald-GruberFriedrich AltmannShahid SiddiqueHolger BohlmannNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Reza Omidvar Nadine Vosseler Amjad Abbas Birgit Gutmann Clemens Grünwald-Gruber Friedrich Altmann Shahid Siddique Holger Bohlmann Analysis of a gene family for PDF-like peptides from Arabidopsis
description	Abstract Plant defensins are small, basic peptides that have a characteristic three-dimensional folding pattern which is stabilized by four disulfide bridges. We show here that Arabidopsis contains in addition to the proper plant defensins a group of 9 plant defensin-like (PdfL) genes. They are all expressed at low levels while GUS fusions of the promoters showed expression in most tissues with only minor differences. We produced two of the encoded peptides in E. coli and tested the antimicrobial activity in vitro. Both were highly active against fungi but had lower activity against bacteria. At higher concentrations hyperbranching and swollen tips, which are indicative of antimicrobial activity, were induced in Fusarium graminearum by both peptides. Overexpression lines for most PdfL genes were produced using the 35S CaMV promoter to study their possible in planta function. With the exception of PdfL4.1 these lines had enhanced resistance against F. oxysporum. All PDFL peptides were also transiently expressed in Nicotiana benthamiana leaves with agroinfiltration using the pPZP3425 vector. In case of PDFL1.4 this resulted in complete death of the infiltrated tissues after 7 days. All other PDFLs resulted only in various degrees of small necrotic lesions. In conclusion, our results show that at least some of the PdfL genes could function in plant resistance.
format	article
author	Reza Omidvar Nadine Vosseler Amjad Abbas Birgit Gutmann Clemens Grünwald-Gruber Friedrich Altmann Shahid Siddique Holger Bohlmann
author_facet	Reza Omidvar Nadine Vosseler Amjad Abbas Birgit Gutmann Clemens Grünwald-Gruber Friedrich Altmann Shahid Siddique Holger Bohlmann
author_sort	Reza Omidvar
title	Analysis of a gene family for PDF-like peptides from Arabidopsis
title_short	Analysis of a gene family for PDF-like peptides from Arabidopsis
title_full	Analysis of a gene family for PDF-like peptides from Arabidopsis
title_fullStr	Analysis of a gene family for PDF-like peptides from Arabidopsis
title_full_unstemmed	Analysis of a gene family for PDF-like peptides from Arabidopsis
title_sort	analysis of a gene family for pdf-like peptides from arabidopsis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/abff19f0cd8d459284a8c7d3c76f6ad2
work_keys_str_mv	AT rezaomidvar analysisofagenefamilyforpdflikepeptidesfromarabidopsis AT nadinevosseler analysisofagenefamilyforpdflikepeptidesfromarabidopsis AT amjadabbas analysisofagenefamilyforpdflikepeptidesfromarabidopsis AT birgitgutmann analysisofagenefamilyforpdflikepeptidesfromarabidopsis AT clemensgrunwaldgruber analysisofagenefamilyforpdflikepeptidesfromarabidopsis AT friedrichaltmann analysisofagenefamilyforpdflikepeptidesfromarabidopsis AT shahidsiddique analysisofagenefamilyforpdflikepeptidesfromarabidopsis AT holgerbohlmann analysisofagenefamilyforpdflikepeptidesfromarabidopsis
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Analysis of a gene family for PDF-like peptides from Arabidopsis

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