Genetic diversity analysis of daluga (Cyrtosperma merkusii) using Sequence-Related Amplified Polymorphism in Siau, Sangihe and Talaud Islands, North Sulawesi, Indonesia

Erlinawati I, Abinawanto, Salamah A, Rugayah. 2018. Genetic diversity analysis of daluga (Cyrtosperma merkusii) using Sequence-Related Amplified Polymorphism in Siau, Sangihe and Talaud Islands, North Sulawesi, Indonesia. Biodiversitas 19: 2374-2380. Daluga or the giant swamp taro (Cyrtosperma merku...

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Autores principales:	INA ERLINAWATI, ABINAWANTO ABINAWANTO, ANDI SALAMAH, RUGAYAH RUGAYAH
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	cyrtosperma merkusii daluga genetic diversity srap Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ac0c63700b9a46f8bf72d7cf8087b7a8
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spelling	oai:doaj.org-article:ac0c63700b9a46f8bf72d7cf8087b7a82021-11-16T14:04:40ZGenetic diversity analysis of daluga (Cyrtosperma merkusii) using Sequence-Related Amplified Polymorphism in Siau, Sangihe and Talaud Islands, North Sulawesi, Indonesia1412-033X2085-472210.13057/biodiv/d190648https://doaj.org/article/ac0c63700b9a46f8bf72d7cf8087b7a82018-10-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3244https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Erlinawati I, Abinawanto, Salamah A, Rugayah. 2018. Genetic diversity analysis of daluga (Cyrtosperma merkusii) using Sequence-Related Amplified Polymorphism in Siau, Sangihe and Talaud Islands, North Sulawesi, Indonesia. Biodiversitas 19: 2374-2380. Daluga or the giant swamp taro (Cyrtosperma merkusii (Hassk.) Schott, a member of Araceae, is one of the minor tuber crops in Indonesia. It has high nutritional value for alternative food. Sequences-Related Amplified Polymorphism (SRAP) primers combinations were used for the first time to assess the genetic diversity among 36 of daluga accessions from several locations in Siau, Sangihe and Talaud islands, North Sulawesi, Indonesia. All of the samples were successfully extracted from silica-gel dried leaves using CTAB methods with minor modifications. Using six SRAP primer combinations, a total of 80 DNA fragments were yielded, varied from 75-1500 bp. The scoring of the fragments resulted in 68 (85.03%) polymorphic bands. Amongst four populations studied, Siau Island population has the highest level of genetic variation with mean values of Na = 1.9375±0.2436, Ne = 1.6409 ± 0.3255, PPL = 93.75%, H = 0.3614 ± 0.1516 and I = 0.5299 ± 0.1978. Meanwhile, Salibabu, Talaud Island population has the lowest level of genetic variation with mean values of Na = 1.5125 ± 0.5030, Ne = 1.3075 ± 0.3723 , PPL = 51.25%, H = 0.1800 ± 0.1989 and I = 0.2700 ± 0.2855. The high of polymorphic bands were generated by SRAP markers suitable for further analysis method in applied and basic of the genetic study on Cyrtosperma species and also related genera. This new genetic information can be used for baseline data for further research especially to improving the variation of daluga and for the conservation of daluga in the future.INA ERLINAWATIABINAWANTO ABINAWANTOANDI SALAMAHRUGAYAH RUGAYAHMBI & UNS Soloarticlecyrtosperma merkusiidalugagenetic diversitysrapBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 6, Pp 2374-2380 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	cyrtosperma merkusii daluga genetic diversity srap Biology (General) QH301-705.5
spellingShingle	cyrtosperma merkusii daluga genetic diversity srap Biology (General) QH301-705.5 INA ERLINAWATI ABINAWANTO ABINAWANTO ANDI SALAMAH RUGAYAH RUGAYAH Genetic diversity analysis of daluga (Cyrtosperma merkusii) using Sequence-Related Amplified Polymorphism in Siau, Sangihe and Talaud Islands, North Sulawesi, Indonesia
description	Erlinawati I, Abinawanto, Salamah A, Rugayah. 2018. Genetic diversity analysis of daluga (Cyrtosperma merkusii) using Sequence-Related Amplified Polymorphism in Siau, Sangihe and Talaud Islands, North Sulawesi, Indonesia. Biodiversitas 19: 2374-2380. Daluga or the giant swamp taro (Cyrtosperma merkusii (Hassk.) Schott, a member of Araceae, is one of the minor tuber crops in Indonesia. It has high nutritional value for alternative food. Sequences-Related Amplified Polymorphism (SRAP) primers combinations were used for the first time to assess the genetic diversity among 36 of daluga accessions from several locations in Siau, Sangihe and Talaud islands, North Sulawesi, Indonesia. All of the samples were successfully extracted from silica-gel dried leaves using CTAB methods with minor modifications. Using six SRAP primer combinations, a total of 80 DNA fragments were yielded, varied from 75-1500 bp. The scoring of the fragments resulted in 68 (85.03%) polymorphic bands. Amongst four populations studied, Siau Island population has the highest level of genetic variation with mean values of Na = 1.9375±0.2436, Ne = 1.6409 ± 0.3255, PPL = 93.75%, H = 0.3614 ± 0.1516 and I = 0.5299 ± 0.1978. Meanwhile, Salibabu, Talaud Island population has the lowest level of genetic variation with mean values of Na = 1.5125 ± 0.5030, Ne = 1.3075 ± 0.3723 , PPL = 51.25%, H = 0.1800 ± 0.1989 and I = 0.2700 ± 0.2855. The high of polymorphic bands were generated by SRAP markers suitable for further analysis method in applied and basic of the genetic study on Cyrtosperma species and also related genera. This new genetic information can be used for baseline data for further research especially to improving the variation of daluga and for the conservation of daluga in the future.
format	article
author	INA ERLINAWATI ABINAWANTO ABINAWANTO ANDI SALAMAH RUGAYAH RUGAYAH
author_facet	INA ERLINAWATI ABINAWANTO ABINAWANTO ANDI SALAMAH RUGAYAH RUGAYAH
author_sort	INA ERLINAWATI
title	Genetic diversity analysis of daluga (Cyrtosperma merkusii) using Sequence-Related Amplified Polymorphism in Siau, Sangihe and Talaud Islands, North Sulawesi, Indonesia
title_short	Genetic diversity analysis of daluga (Cyrtosperma merkusii) using Sequence-Related Amplified Polymorphism in Siau, Sangihe and Talaud Islands, North Sulawesi, Indonesia
title_full	Genetic diversity analysis of daluga (Cyrtosperma merkusii) using Sequence-Related Amplified Polymorphism in Siau, Sangihe and Talaud Islands, North Sulawesi, Indonesia
title_fullStr	Genetic diversity analysis of daluga (Cyrtosperma merkusii) using Sequence-Related Amplified Polymorphism in Siau, Sangihe and Talaud Islands, North Sulawesi, Indonesia
title_full_unstemmed	Genetic diversity analysis of daluga (Cyrtosperma merkusii) using Sequence-Related Amplified Polymorphism in Siau, Sangihe and Talaud Islands, North Sulawesi, Indonesia
title_sort	genetic diversity analysis of daluga (cyrtosperma merkusii) using sequence-related amplified polymorphism in siau, sangihe and talaud islands, north sulawesi, indonesia
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/ac0c63700b9a46f8bf72d7cf8087b7a8
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Genetic diversity analysis of daluga (Cyrtosperma merkusii) using Sequence-Related Amplified Polymorphism in Siau, Sangihe and Talaud Islands, North Sulawesi, Indonesia

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