Morphological and Transcriptional Responses to CRISPRi Knockdown of Essential Genes in Escherichia coli

Essential genes make up only ∼5 to 10% of the genetic complement in most organisms but occupy much of their protein synthesis and account for almost all antibiotic targets. Despite the importance of essential genes, their intractability has, until recently, hampered efforts to study them.

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Autores principales:	Melanie R. Silvis, Manohary Rajendram, Handuo Shi, Hendrik Osadnik, Andrew N. Gray, Spencer Cesar, Jason M. Peters, Cameron C. Hearne, Parth Kumar, Horia Todor, Kerwyn Casey Huang, Carol A. Gross
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2021
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/ac0ea87fcbd444e6a5571025f19f94b5
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spelling	oai:doaj.org-article:ac0ea87fcbd444e6a5571025f19f94b52021-11-03T21:52:35ZMorphological and Transcriptional Responses to CRISPRi Knockdown of Essential Genes in Escherichia coli2150-751110.1128/mBio.02561-21https://doaj.org/article/ac0ea87fcbd444e6a5571025f19f94b52021-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.02561-21https://doaj.org/toc/2150-7511 Essential genes make up only ∼5 to 10% of the genetic complement in most organisms but occupy much of their protein synthesis and account for almost all antibiotic targets. Despite the importance of essential genes, their intractability has, until recently, hampered efforts to study them.Melanie R. SilvisManohary RajendramHanduo ShiHendrik OsadnikAndrew N. GraySpencer CesarJason M. PetersCameron C. HearneParth KumarHoria TodorKerwyn Casey HuangCarol A. GrossAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 12, Iss 5 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Melanie R. Silvis Manohary Rajendram Handuo Shi Hendrik Osadnik Andrew N. Gray Spencer Cesar Jason M. Peters Cameron C. Hearne Parth Kumar Horia Todor Kerwyn Casey Huang Carol A. Gross Morphological and Transcriptional Responses to CRISPRi Knockdown of Essential Genes in Escherichia coli
description	Essential genes make up only ∼5 to 10% of the genetic complement in most organisms but occupy much of their protein synthesis and account for almost all antibiotic targets. Despite the importance of essential genes, their intractability has, until recently, hampered efforts to study them.
format	article
author	Melanie R. Silvis Manohary Rajendram Handuo Shi Hendrik Osadnik Andrew N. Gray Spencer Cesar Jason M. Peters Cameron C. Hearne Parth Kumar Horia Todor Kerwyn Casey Huang Carol A. Gross
author_facet	Melanie R. Silvis Manohary Rajendram Handuo Shi Hendrik Osadnik Andrew N. Gray Spencer Cesar Jason M. Peters Cameron C. Hearne Parth Kumar Horia Todor Kerwyn Casey Huang Carol A. Gross
author_sort	Melanie R. Silvis
title	Morphological and Transcriptional Responses to CRISPRi Knockdown of Essential Genes in Escherichia coli
title_short	Morphological and Transcriptional Responses to CRISPRi Knockdown of Essential Genes in Escherichia coli
title_full	Morphological and Transcriptional Responses to CRISPRi Knockdown of Essential Genes in Escherichia coli
title_fullStr	Morphological and Transcriptional Responses to CRISPRi Knockdown of Essential Genes in Escherichia coli
title_full_unstemmed	Morphological and Transcriptional Responses to CRISPRi Knockdown of Essential Genes in Escherichia coli
title_sort	morphological and transcriptional responses to crispri knockdown of essential genes in escherichia coli
publisher	American Society for Microbiology
publishDate	2021
url	https://doaj.org/article/ac0ea87fcbd444e6a5571025f19f94b5
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Morphological and Transcriptional Responses to CRISPRi Knockdown of Essential Genes in Escherichia coli

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