Physical Binding of Endothelial MCAM and Neural Transmembrane Protease Matriptase—Novel Cell Adhesion in Neural Stem cell Vascular Niche

Abstract Brain neural stem cells and transit amplifying cells in the subventricular zone (SVZ) of the lateral ventricles are in direct contact with the microvascular endothelium. The mechanisms/molecules of direct cell contact in the SVZ neurovascular niche are not fully understood. We previously sh...

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Autores principales:	Hsiu-Hui Tung, Sheau-Ling Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ac19b7711d544673a880389e0d22e760
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spelling	oai:doaj.org-article:ac19b7711d544673a880389e0d22e7602021-12-02T11:52:23ZPhysical Binding of Endothelial MCAM and Neural Transmembrane Protease Matriptase—Novel Cell Adhesion in Neural Stem cell Vascular Niche10.1038/s41598-017-05131-42045-2322https://doaj.org/article/ac19b7711d544673a880389e0d22e7602017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05131-4https://doaj.org/toc/2045-2322Abstract Brain neural stem cells and transit amplifying cells in the subventricular zone (SVZ) of the lateral ventricles are in direct contact with the microvascular endothelium. The mechanisms/molecules of direct cell contact in the SVZ neurovascular niche are not fully understood. We previously showed that neural stem/progenitor (NS/P) cells induce brain endothelial signaling in direct cell contact through matriptase (MTP) on NS/P cell surface. In the present study, using pull-down and LC-MS/MS, we identified melanoma cell adhesion molecule (MCAM) the brain endothelial molecule that interacts with MTP. MCAM physically binds to the CUB domains of MTP and induces a chain of brain endothelial signaling including p38MAPK activation, GSK3β inactivation and subsequently β-catenin activation; none of these signaling events occurred when either MTP or MCAM is deleted. MTP-MCAM binding and induction of endothelial signaling were all sensitive to cholera toxin. Together, we identified key molecules that may represent a mechanism in neural stem cell vascular niche regulation.Hsiu-Hui TungSheau-Ling LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hsiu-Hui Tung Sheau-Ling Lee Physical Binding of Endothelial MCAM and Neural Transmembrane Protease Matriptase—Novel Cell Adhesion in Neural Stem cell Vascular Niche
description	Abstract Brain neural stem cells and transit amplifying cells in the subventricular zone (SVZ) of the lateral ventricles are in direct contact with the microvascular endothelium. The mechanisms/molecules of direct cell contact in the SVZ neurovascular niche are not fully understood. We previously showed that neural stem/progenitor (NS/P) cells induce brain endothelial signaling in direct cell contact through matriptase (MTP) on NS/P cell surface. In the present study, using pull-down and LC-MS/MS, we identified melanoma cell adhesion molecule (MCAM) the brain endothelial molecule that interacts with MTP. MCAM physically binds to the CUB domains of MTP and induces a chain of brain endothelial signaling including p38MAPK activation, GSK3β inactivation and subsequently β-catenin activation; none of these signaling events occurred when either MTP or MCAM is deleted. MTP-MCAM binding and induction of endothelial signaling were all sensitive to cholera toxin. Together, we identified key molecules that may represent a mechanism in neural stem cell vascular niche regulation.
format	article
author	Hsiu-Hui Tung Sheau-Ling Lee
author_facet	Hsiu-Hui Tung Sheau-Ling Lee
author_sort	Hsiu-Hui Tung
title	Physical Binding of Endothelial MCAM and Neural Transmembrane Protease Matriptase—Novel Cell Adhesion in Neural Stem cell Vascular Niche
title_short	Physical Binding of Endothelial MCAM and Neural Transmembrane Protease Matriptase—Novel Cell Adhesion in Neural Stem cell Vascular Niche
title_full	Physical Binding of Endothelial MCAM and Neural Transmembrane Protease Matriptase—Novel Cell Adhesion in Neural Stem cell Vascular Niche
title_fullStr	Physical Binding of Endothelial MCAM and Neural Transmembrane Protease Matriptase—Novel Cell Adhesion in Neural Stem cell Vascular Niche
title_full_unstemmed	Physical Binding of Endothelial MCAM and Neural Transmembrane Protease Matriptase—Novel Cell Adhesion in Neural Stem cell Vascular Niche
title_sort	physical binding of endothelial mcam and neural transmembrane protease matriptase—novel cell adhesion in neural stem cell vascular niche
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ac19b7711d544673a880389e0d22e760
work_keys_str_mv	AT hsiuhuitung physicalbindingofendothelialmcamandneuraltransmembraneproteasematriptasenovelcelladhesioninneuralstemcellvascularniche AT sheaulinglee physicalbindingofendothelialmcamandneuraltransmembraneproteasematriptasenovelcelladhesioninneuralstemcellvascularniche
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Physical Binding of Endothelial MCAM and Neural Transmembrane Protease Matriptase—Novel Cell Adhesion in Neural Stem cell Vascular Niche

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