Metabolite Profiling of Tabat Barito (Ficus deltoidea) Using UPLC-QTOF-MS/MS

Tabat Barito (Ficus deltoidea) is known as a plant that has potency as an antioxidant because of containing a significant phenolic compound. In this study, we performed metabolite profiling on F. deltoidea leaves by UPLC-QTOF-MS/MS to identify its phenolic compounds. Extraction by maceration and ult...

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Autores principales:	Nurmaida Nurmaida, Latifah K Darusman, Mohamad Rafi, Rudi Heryanto
Formato:	article
Lenguaje:	EN
Publicado:	University of Brawijaya 2018
Materias:	ficus deltoidea metabolite profiling uplc-qtof-ms/ms antioxidant activity Chemistry QD1-999
Acceso en línea:	http://dx.doi.org/10.21776/ub.jpacr.2018.007.02.374 https://doaj.org/article/ac2578c3f2a54b9f94ebb51df98b2f14
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spelling	oai:doaj.org-article:ac2578c3f2a54b9f94ebb51df98b2f142021-12-02T14:11:07ZMetabolite Profiling of Tabat Barito (Ficus deltoidea) Using UPLC-QTOF-MS/MShttp://dx.doi.org/10.21776/ub.jpacr.2018.007.02.3742302-46902302-4690https://doaj.org/article/ac2578c3f2a54b9f94ebb51df98b2f142018-05-01T00:00:00Zhttps://jpacr.ub.ac.id/index.php/jpacr/article/view/374/pdfhttps://doaj.org/toc/2302-4690https://doaj.org/toc/2302-4690Tabat Barito (Ficus deltoidea) is known as a plant that has potency as an antioxidant because of containing a significant phenolic compound. In this study, we performed metabolite profiling on F. deltoidea leaves by UPLC-QTOF-MS/MS to identify its phenolic compounds. Extraction by maceration and ultrasonication techniques with methanol (MM and UM) and ethanol (ME and UE) were used to extract the F. deltoidea metabolites. About 70 metabolites were identified by using UPLC-QTOF-MS/MS in negative ion mode. The amounts of metabolites found in each extract were different, i.e., 45 metabolites in MM, 64 metabolites in UM, 42 metabolites in UE and 41 metabolites in ME. MS/MS could further tentatively identify 16 metabolites. The identified compounds belonged to the class of flavonoids and phenolic acid. Also, we conducted an antioxidant activity by using DPPH method on each extract to determine its potency as an antioxidant. The highest antioxidant activity was exhibited by UM extract (IC50 71.93 ppm) may be due to the number of metabolites in UM extract which was higher than the other extract based on the detected metabolites.Nurmaida NurmaidaLatifah K DarusmanMohamad RafiRudi HeryantoUniversity of Brawijayaarticleficus deltoideametabolite profilinguplc-qtof-ms/msantioxidant activityChemistryQD1-999ENJournal of Pure and Applied Chemistry Research, Vol 7, Iss 2, Pp 100-108 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	ficus deltoidea metabolite profiling uplc-qtof-ms/ms antioxidant activity Chemistry QD1-999
spellingShingle	ficus deltoidea metabolite profiling uplc-qtof-ms/ms antioxidant activity Chemistry QD1-999 Nurmaida Nurmaida Latifah K Darusman Mohamad Rafi Rudi Heryanto Metabolite Profiling of Tabat Barito (Ficus deltoidea) Using UPLC-QTOF-MS/MS
description	Tabat Barito (Ficus deltoidea) is known as a plant that has potency as an antioxidant because of containing a significant phenolic compound. In this study, we performed metabolite profiling on F. deltoidea leaves by UPLC-QTOF-MS/MS to identify its phenolic compounds. Extraction by maceration and ultrasonication techniques with methanol (MM and UM) and ethanol (ME and UE) were used to extract the F. deltoidea metabolites. About 70 metabolites were identified by using UPLC-QTOF-MS/MS in negative ion mode. The amounts of metabolites found in each extract were different, i.e., 45 metabolites in MM, 64 metabolites in UM, 42 metabolites in UE and 41 metabolites in ME. MS/MS could further tentatively identify 16 metabolites. The identified compounds belonged to the class of flavonoids and phenolic acid. Also, we conducted an antioxidant activity by using DPPH method on each extract to determine its potency as an antioxidant. The highest antioxidant activity was exhibited by UM extract (IC50 71.93 ppm) may be due to the number of metabolites in UM extract which was higher than the other extract based on the detected metabolites.
format	article
author	Nurmaida Nurmaida Latifah K Darusman Mohamad Rafi Rudi Heryanto
author_facet	Nurmaida Nurmaida Latifah K Darusman Mohamad Rafi Rudi Heryanto
author_sort	Nurmaida Nurmaida
title	Metabolite Profiling of Tabat Barito (Ficus deltoidea) Using UPLC-QTOF-MS/MS
title_short	Metabolite Profiling of Tabat Barito (Ficus deltoidea) Using UPLC-QTOF-MS/MS
title_full	Metabolite Profiling of Tabat Barito (Ficus deltoidea) Using UPLC-QTOF-MS/MS
title_fullStr	Metabolite Profiling of Tabat Barito (Ficus deltoidea) Using UPLC-QTOF-MS/MS
title_full_unstemmed	Metabolite Profiling of Tabat Barito (Ficus deltoidea) Using UPLC-QTOF-MS/MS
title_sort	metabolite profiling of tabat barito (ficus deltoidea) using uplc-qtof-ms/ms
publisher	University of Brawijaya
publishDate	2018
url	http://dx.doi.org/10.21776/ub.jpacr.2018.007.02.374 https://doaj.org/article/ac2578c3f2a54b9f94ebb51df98b2f14
work_keys_str_mv	AT nurmaidanurmaida metaboliteprofilingoftabatbaritoficusdeltoideausinguplcqtofmsms AT latifahkdarusman metaboliteprofilingoftabatbaritoficusdeltoideausinguplcqtofmsms AT mohamadrafi metaboliteprofilingoftabatbaritoficusdeltoideausinguplcqtofmsms AT rudiheryanto metaboliteprofilingoftabatbaritoficusdeltoideausinguplcqtofmsms
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Metabolite Profiling of Tabat Barito (Ficus deltoidea) Using UPLC-QTOF-MS/MS

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