Impact of surface coating and particle size on the uptake of small and ultrasmall superparamagnetic iron oxide nanoparticles by macrophages

Shigeyoshi Saito,1 Mana Tsugeno,1 Daichi Koto,1 Yuki Mori,2 Yoshichika Yoshioka,2 Satoshi Nohara,3 Kenya Murase11Department of Medical Physics and Engineering, Division of Medical Technology and Science, Faculty of Health Science, Graduate School of Medicine, 2Biofunctional Imaging Lab, Immunology F...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Saito S, Tsugeno M, Koto D, Mori Y, Yoshioka Y, Nohara S, Murase K
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ac27276e4c244d7696cc539c303116c3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ac27276e4c244d7696cc539c303116c3
record_format	dspace
spelling	oai:doaj.org-article:ac27276e4c244d7696cc539c303116c32021-12-02T01:19:12ZImpact of surface coating and particle size on the uptake of small and ultrasmall superparamagnetic iron oxide nanoparticles by macrophages1176-91141178-2013https://doaj.org/article/ac27276e4c244d7696cc539c303116c32012-10-01T00:00:00Zhttp://www.dovepress.com/impact-of-surface-coating-and-particle-size-on-the-uptake-of-small-and-a11227https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Shigeyoshi Saito,1 Mana Tsugeno,1 Daichi Koto,1 Yuki Mori,2 Yoshichika Yoshioka,2 Satoshi Nohara,3 Kenya Murase11Department of Medical Physics and Engineering, Division of Medical Technology and Science, Faculty of Health Science, Graduate School of Medicine, 2Biofunctional Imaging Lab, Immunology Frontier Research Center (WPI-IFReC), Osaka University, Osaka, Japan; 3The Nagoya Research Laboratory, Meito Sangyo Co, Ltd, Kiyosu, Aichi, JapanPurpose: Magnetic resonance imaging (MRI) using contrast agents like superparamagnetic iron oxide (SPIO) is an extremely versatile technique to diagnose diseases and to monitor treatment. This study tested the relative importance of particle size and surface coating for the optimization of MRI contrast and labeling efficiency of macrophages migrating to remote inflammation sites.Materials and methods: We tested four SPIO and ultrasmall superparamagnetic iron oxide (USPIO), alkali-treated dextran magnetite (ATDM) with particle sizes of 28 and 74 nm, and carboxymethyl dextran magnetite (CMDM) with particle sizes of 28 and 72 nm. Mouse macrophage RAW264 cells were incubated with SPIOs and USPIOs, and the labeling efficiency of the cells was determined by the percentage of Berlin blue-stained cells and by measuring T2 relaxation times with 11.7-T MRI. We used trypan blue staining to measure cell viability.Results: Analysis of the properties of the nanoparticles revealed that ATDM-coated 74 nm particles have a lower T2 relaxation time than the others, translating into a higher ability of MRI negative contrast agent. Among the other three candidates, CMDM-coated particles showed the highest T2 relaxation time once internalized by macrophages. Regarding labeling efficiency, ATDM coating resulted in a cellular uptake higher than CMDM coating, independent of nanoparticle size. None of these particle formulations affected macrophage viability.Conclusion: This study suggests that coating is more critical than size to optimize the SPIO labeling of macrophages. Among the formulations tested in this study, the best MRI contrast and labeling efficiency are expected with ATDM-coated 74 nm nanoparticles.Keywords: ultrasmall superparamagnetic iron oxide, cultured mouse macrophage cells, surface coating, particle size, MRI&nbsp;Saito STsugeno MKoto DMori YYoshioka YNohara SMurase KDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 5415-5421 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Saito S Tsugeno M Koto D Mori Y Yoshioka Y Nohara S Murase K Impact of surface coating and particle size on the uptake of small and ultrasmall superparamagnetic iron oxide nanoparticles by macrophages
description	Shigeyoshi Saito,1 Mana Tsugeno,1 Daichi Koto,1 Yuki Mori,2 Yoshichika Yoshioka,2 Satoshi Nohara,3 Kenya Murase11Department of Medical Physics and Engineering, Division of Medical Technology and Science, Faculty of Health Science, Graduate School of Medicine, 2Biofunctional Imaging Lab, Immunology Frontier Research Center (WPI-IFReC), Osaka University, Osaka, Japan; 3The Nagoya Research Laboratory, Meito Sangyo Co, Ltd, Kiyosu, Aichi, JapanPurpose: Magnetic resonance imaging (MRI) using contrast agents like superparamagnetic iron oxide (SPIO) is an extremely versatile technique to diagnose diseases and to monitor treatment. This study tested the relative importance of particle size and surface coating for the optimization of MRI contrast and labeling efficiency of macrophages migrating to remote inflammation sites.Materials and methods: We tested four SPIO and ultrasmall superparamagnetic iron oxide (USPIO), alkali-treated dextran magnetite (ATDM) with particle sizes of 28 and 74 nm, and carboxymethyl dextran magnetite (CMDM) with particle sizes of 28 and 72 nm. Mouse macrophage RAW264 cells were incubated with SPIOs and USPIOs, and the labeling efficiency of the cells was determined by the percentage of Berlin blue-stained cells and by measuring T2 relaxation times with 11.7-T MRI. We used trypan blue staining to measure cell viability.Results: Analysis of the properties of the nanoparticles revealed that ATDM-coated 74 nm particles have a lower T2 relaxation time than the others, translating into a higher ability of MRI negative contrast agent. Among the other three candidates, CMDM-coated particles showed the highest T2 relaxation time once internalized by macrophages. Regarding labeling efficiency, ATDM coating resulted in a cellular uptake higher than CMDM coating, independent of nanoparticle size. None of these particle formulations affected macrophage viability.Conclusion: This study suggests that coating is more critical than size to optimize the SPIO labeling of macrophages. Among the formulations tested in this study, the best MRI contrast and labeling efficiency are expected with ATDM-coated 74 nm nanoparticles.Keywords: ultrasmall superparamagnetic iron oxide, cultured mouse macrophage cells, surface coating, particle size, MRI&nbsp;
format	article
author	Saito S Tsugeno M Koto D Mori Y Yoshioka Y Nohara S Murase K
author_facet	Saito S Tsugeno M Koto D Mori Y Yoshioka Y Nohara S Murase K
author_sort	Saito S
title	Impact of surface coating and particle size on the uptake of small and ultrasmall superparamagnetic iron oxide nanoparticles by macrophages
title_short	Impact of surface coating and particle size on the uptake of small and ultrasmall superparamagnetic iron oxide nanoparticles by macrophages
title_full	Impact of surface coating and particle size on the uptake of small and ultrasmall superparamagnetic iron oxide nanoparticles by macrophages
title_fullStr	Impact of surface coating and particle size on the uptake of small and ultrasmall superparamagnetic iron oxide nanoparticles by macrophages
title_full_unstemmed	Impact of surface coating and particle size on the uptake of small and ultrasmall superparamagnetic iron oxide nanoparticles by macrophages
title_sort	impact of surface coating and particle size on the uptake of small and ultrasmall superparamagnetic iron oxide nanoparticles by macrophages
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/ac27276e4c244d7696cc539c303116c3
work_keys_str_mv	AT saitos impactofsurfacecoatingandparticlesizeontheuptakeofsmallandultrasmallsuperparamagneticironoxidenanoparticlesbymacrophages AT tsugenom impactofsurfacecoatingandparticlesizeontheuptakeofsmallandultrasmallsuperparamagneticironoxidenanoparticlesbymacrophages AT kotod impactofsurfacecoatingandparticlesizeontheuptakeofsmallandultrasmallsuperparamagneticironoxidenanoparticlesbymacrophages AT moriy impactofsurfacecoatingandparticlesizeontheuptakeofsmallandultrasmallsuperparamagneticironoxidenanoparticlesbymacrophages AT yoshiokay impactofsurfacecoatingandparticlesizeontheuptakeofsmallandultrasmallsuperparamagneticironoxidenanoparticlesbymacrophages AT noharas impactofsurfacecoatingandparticlesizeontheuptakeofsmallandultrasmallsuperparamagneticironoxidenanoparticlesbymacrophages AT murasek impactofsurfacecoatingandparticlesizeontheuptakeofsmallandultrasmallsuperparamagneticironoxidenanoparticlesbymacrophages
_version_	1718403156654686208

Impact of surface coating and particle size on the uptake of small and ultrasmall superparamagnetic iron oxide nanoparticles by macrophages

Ejemplares similares