Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells

Background: Osteoarthritis is a complex degenerative disease with several factors contributing to joint damage. Objective: To compare the potential effect of hyaluronic acid (HA) and triamcinolone acetonide (TA), alone or combined, on the in vitro chondrogenic differentiation process of mesenchymal...

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Autores principales:	Pablo E. Ocampo, Viviana Vallejo, Luis M. Montoya, Noeme S. Rocha, Fernanda da C. Landim, Sheila C. Rahal
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2020
Materias:	cell differentiation cartilage chondrocytes chondrogenesis chondrogenic differentiation collagen corticosteroids glucocorticoid histology hyaluronic acid mesenchymal stem cell osteoarthritis triancinolone acetonide viscosupplementation Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/ac359c6d52d846f4a3bcbffe4dc77ed9
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spelling	oai:doaj.org-article:ac359c6d52d846f4a3bcbffe4dc77ed92021-12-01T15:02:45ZPotential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells2256-295810.17533/udea.rccp.v34n3a06https://doaj.org/article/ac359c6d52d846f4a3bcbffe4dc77ed92020-09-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/rccp/article/view/343953https://doaj.org/toc/2256-2958Background: Osteoarthritis is a complex degenerative disease with several factors contributing to joint damage. Objective: To compare the potential effect of hyaluronic acid (HA) and triamcinolone acetonide (TA), alone or combined, on the in vitro chondrogenic differentiation process of mesenchymal stem cells (MSCs). Methods: MSCs were divided into four groups: Control, HA, TA, and HA/TA combined. Each treatment group was cultured for 14 days in chondrogenic differentiation medium. The chondrogenic differentiation potential was assessed by histology and immunohistochemistry. Results: The HA and HA/TA-treated MSCs presented histological characteristics similar to native chondrocytes. The extracellular matrix (ECM) of TA-treated MSCs was compact and organized. Glycosaminoglycan staining was intense in Control, moderate in TA, slight in HA/TA, and undetectable in HA. Type II collagen immunoreactivity was high in the TA-treated ECM and MSCs. Conclusions: Histological analysis shows that HA influences morphological development similar to chondrocytes of the MSCs, but with low expression of specific cartilage molecules. The TA promotes formation of a compact and organized ECM.Pablo E. OcampoViviana VallejoLuis M. MontoyaNoeme S. RochaFernanda da C. LandimSheila C. RahalUniversidad de Antioquiaarticlecell differentiationcartilagechondrocyteschondrogenesischondrogenic differentiationcollagencorticosteroidsglucocorticoidhistologyhyaluronic acidmesenchymal stem cellosteoarthritistriancinolone acetonideviscosupplementationAnimal cultureSF1-1100ENRevista Colombiana de Ciencias Pecuarias, Vol 34, Iss 3, Pp 212-223 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	cell differentiation cartilage chondrocytes chondrogenesis chondrogenic differentiation collagen corticosteroids glucocorticoid histology hyaluronic acid mesenchymal stem cell osteoarthritis triancinolone acetonide viscosupplementation Animal culture SF1-1100
spellingShingle	cell differentiation cartilage chondrocytes chondrogenesis chondrogenic differentiation collagen corticosteroids glucocorticoid histology hyaluronic acid mesenchymal stem cell osteoarthritis triancinolone acetonide viscosupplementation Animal culture SF1-1100 Pablo E. Ocampo Viviana Vallejo Luis M. Montoya Noeme S. Rocha Fernanda da C. Landim Sheila C. Rahal Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells
description	Background: Osteoarthritis is a complex degenerative disease with several factors contributing to joint damage. Objective: To compare the potential effect of hyaluronic acid (HA) and triamcinolone acetonide (TA), alone or combined, on the in vitro chondrogenic differentiation process of mesenchymal stem cells (MSCs). Methods: MSCs were divided into four groups: Control, HA, TA, and HA/TA combined. Each treatment group was cultured for 14 days in chondrogenic differentiation medium. The chondrogenic differentiation potential was assessed by histology and immunohistochemistry. Results: The HA and HA/TA-treated MSCs presented histological characteristics similar to native chondrocytes. The extracellular matrix (ECM) of TA-treated MSCs was compact and organized. Glycosaminoglycan staining was intense in Control, moderate in TA, slight in HA/TA, and undetectable in HA. Type II collagen immunoreactivity was high in the TA-treated ECM and MSCs. Conclusions: Histological analysis shows that HA influences morphological development similar to chondrocytes of the MSCs, but with low expression of specific cartilage molecules. The TA promotes formation of a compact and organized ECM.
format	article
author	Pablo E. Ocampo Viviana Vallejo Luis M. Montoya Noeme S. Rocha Fernanda da C. Landim Sheila C. Rahal
author_facet	Pablo E. Ocampo Viviana Vallejo Luis M. Montoya Noeme S. Rocha Fernanda da C. Landim Sheila C. Rahal
author_sort	Pablo E. Ocampo
title	Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells
title_short	Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells
title_full	Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells
title_fullStr	Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells
title_full_unstemmed	Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells
title_sort	potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells
publisher	Universidad de Antioquia
publishDate	2020
url	https://doaj.org/article/ac359c6d52d846f4a3bcbffe4dc77ed9
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Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells

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