An Oligomannuronic Acid-Sialic Acid Conjugate Capable of Inhibiting Aβ42 Aggregation and Alleviating the Inflammatory Response of BV-2 Microglia
Oligomannuronic acid (MOS) from seaweed has antioxidant and anti-inflammatory activities. In this study, MOS was activated at the terminal to obtain three different graft complexes modified with sialic acid moiety (MOS-Sia). The results show that MOS-Sia addition can reduce the β-structure formation...
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2021
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oai:doaj.org-article:ac38baa10c9249f0b30fe5bcf158bc482021-11-25T17:55:31ZAn Oligomannuronic Acid-Sialic Acid Conjugate Capable of Inhibiting Aβ42 Aggregation and Alleviating the Inflammatory Response of BV-2 Microglia10.3390/ijms2222123381422-00671661-6596https://doaj.org/article/ac38baa10c9249f0b30fe5bcf158bc482021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12338https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Oligomannuronic acid (MOS) from seaweed has antioxidant and anti-inflammatory activities. In this study, MOS was activated at the terminal to obtain three different graft complexes modified with sialic acid moiety (MOS-Sia). The results show that MOS-Sia addition can reduce the β-structure formation of Aβ42, and the binding effect of MOS-Sia3 is more obvious. MOS-Sia conjugates also have a better complexing effect with Ca<sup>2+</sup> while reducing the formation of Aβ42 oligomers in solutions. MOS-Sia3 (25–50 μg/mL) can effectively inhibit the activation state of BV-2 cells stimulated by Aβ42, whereas a higher dose of MOS-Sia3 (>50 μg/mL) can inhibit the proliferation of BV-2 cells to a certain extent. A lower dose of MOS-Sia3 can also inhibit the expression of IL-1β, IL-6, TNF-α, and other proinflammatory factors in BV-2 cells induced by Aβ42 activation. In the future, the MOS-Sia3 conjugate can be used to treat Alzheimer’s disease.Jianrong WuMiaosen WuHongtao ZhangXiaobei ZhanNian WuMDPI AGarticleoligomannuronic acidsialic acidAlzheimer’s diseaseβ-amyloidmicrogliaBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12338, p 12338 (2021) |
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DOAJ |
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DOAJ |
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| topic |
oligomannuronic acid sialic acid Alzheimer’s disease β-amyloid microglia Biology (General) QH301-705.5 Chemistry QD1-999 |
| spellingShingle |
oligomannuronic acid sialic acid Alzheimer’s disease β-amyloid microglia Biology (General) QH301-705.5 Chemistry QD1-999 Jianrong Wu Miaosen Wu Hongtao Zhang Xiaobei Zhan Nian Wu An Oligomannuronic Acid-Sialic Acid Conjugate Capable of Inhibiting Aβ42 Aggregation and Alleviating the Inflammatory Response of BV-2 Microglia |
| description |
Oligomannuronic acid (MOS) from seaweed has antioxidant and anti-inflammatory activities. In this study, MOS was activated at the terminal to obtain three different graft complexes modified with sialic acid moiety (MOS-Sia). The results show that MOS-Sia addition can reduce the β-structure formation of Aβ42, and the binding effect of MOS-Sia3 is more obvious. MOS-Sia conjugates also have a better complexing effect with Ca<sup>2+</sup> while reducing the formation of Aβ42 oligomers in solutions. MOS-Sia3 (25–50 μg/mL) can effectively inhibit the activation state of BV-2 cells stimulated by Aβ42, whereas a higher dose of MOS-Sia3 (>50 μg/mL) can inhibit the proliferation of BV-2 cells to a certain extent. A lower dose of MOS-Sia3 can also inhibit the expression of IL-1β, IL-6, TNF-α, and other proinflammatory factors in BV-2 cells induced by Aβ42 activation. In the future, the MOS-Sia3 conjugate can be used to treat Alzheimer’s disease. |
| format |
article |
| author |
Jianrong Wu Miaosen Wu Hongtao Zhang Xiaobei Zhan Nian Wu |
| author_facet |
Jianrong Wu Miaosen Wu Hongtao Zhang Xiaobei Zhan Nian Wu |
| author_sort |
Jianrong Wu |
| title |
An Oligomannuronic Acid-Sialic Acid Conjugate Capable of Inhibiting Aβ42 Aggregation and Alleviating the Inflammatory Response of BV-2 Microglia |
| title_short |
An Oligomannuronic Acid-Sialic Acid Conjugate Capable of Inhibiting Aβ42 Aggregation and Alleviating the Inflammatory Response of BV-2 Microglia |
| title_full |
An Oligomannuronic Acid-Sialic Acid Conjugate Capable of Inhibiting Aβ42 Aggregation and Alleviating the Inflammatory Response of BV-2 Microglia |
| title_fullStr |
An Oligomannuronic Acid-Sialic Acid Conjugate Capable of Inhibiting Aβ42 Aggregation and Alleviating the Inflammatory Response of BV-2 Microglia |
| title_full_unstemmed |
An Oligomannuronic Acid-Sialic Acid Conjugate Capable of Inhibiting Aβ42 Aggregation and Alleviating the Inflammatory Response of BV-2 Microglia |
| title_sort |
oligomannuronic acid-sialic acid conjugate capable of inhibiting aβ42 aggregation and alleviating the inflammatory response of bv-2 microglia |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/ac38baa10c9249f0b30fe5bcf158bc48 |
| work_keys_str_mv |
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| _version_ |
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