Nutrient-related metabolite profiles explain differences in body composition and size in Nile tilapia (Oreochromis niloticus) from different lakes

Abstract This study investigated how metabolite analysis can explain differences in tissue composition and size in fish from different habitats. We, therefore, studied Nile tilapia (Oreochromis niloticus) from three Ethiopian lakes (Gilgel Gibe, Ziway, and Langano) using dried bloodspot (DBS) analys...

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Autores principales:	Tokuma Negisho Bayissa, Michelle Geerardyn, Donna Vanhauteghem, Mulugeta Wakjira, Geert Paul Jules Janssens
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ac3af859f35b4bdf92d6ec4ebfc337cf
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spelling	oai:doaj.org-article:ac3af859f35b4bdf92d6ec4ebfc337cf2021-12-02T16:45:54ZNutrient-related metabolite profiles explain differences in body composition and size in Nile tilapia (Oreochromis niloticus) from different lakes10.1038/s41598-021-96326-32045-2322https://doaj.org/article/ac3af859f35b4bdf92d6ec4ebfc337cf2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96326-3https://doaj.org/toc/2045-2322Abstract This study investigated how metabolite analysis can explain differences in tissue composition and size in fish from different habitats. We, therefore, studied Nile tilapia (Oreochromis niloticus) from three Ethiopian lakes (Gilgel Gibe, Ziway, and Langano) using dried bloodspot (DBS) analysis of carnitine esters and free amino acids. A total of sixty (N = 60) Nile tilapia samples were collected comprising twenty (n = 20) fish from each lake. The proximate composition of the targeted tissues (muscle, skin, gill, gut, and liver) were analyzed. The DBS samples were analyzed for acylcarnitine and free amino acid profiles using quantitative electrospray tandem mass spectrometry. Metabolite ratios were calculated from relevant biochemical pathways that could identify relative changes in nutrient metabolism. The mean weight of Nile tilapia sampled from each lake showed weight variation among the lakes, fish from Lake Ziway were largest (178 g), followed by Gilgel Gibe reservoir (134 g) and Lake Langano (118 g). Fish from Gilgel Gibe showed significantly higher fat composition in all tissues (P < 0.05) except the liver in which no significant variation was observed. The source of fish affected the tissue fat composition. Marked differences were observed in Nile tilapia metabolic activity between the lakes. For instance, the lower body weight and condition of the fish in Lake Langano coincided with several metabolite ratios pointing to a low flow of glucogenic substrate to the citric acid cycle. The low propionyl to acetylcarnitine ratio (C3:C2) in Gilgel Gibe fish is indicating that more of the available acetyl CoA is not led into the citric acid cycle, but instead will be used for fat synthesis. The metabolic markers for lipogenesis and metabolic rate could explain the high-fat concentration in several parts of the body composition of fish from Gilgel Gibe. Our results show that nutrition-related blood metabolite ratios are useful to understand the underlying metabolic events leading to the habitat-dependent differences in the growth of Nile tilapia, and by extension, other species.Tokuma Negisho BayissaMichelle GeerardynDonna VanhauteghemMulugeta WakjiraGeert Paul Jules JanssensNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tokuma Negisho Bayissa Michelle Geerardyn Donna Vanhauteghem Mulugeta Wakjira Geert Paul Jules Janssens Nutrient-related metabolite profiles explain differences in body composition and size in Nile tilapia (Oreochromis niloticus) from different lakes
description	Abstract This study investigated how metabolite analysis can explain differences in tissue composition and size in fish from different habitats. We, therefore, studied Nile tilapia (Oreochromis niloticus) from three Ethiopian lakes (Gilgel Gibe, Ziway, and Langano) using dried bloodspot (DBS) analysis of carnitine esters and free amino acids. A total of sixty (N = 60) Nile tilapia samples were collected comprising twenty (n = 20) fish from each lake. The proximate composition of the targeted tissues (muscle, skin, gill, gut, and liver) were analyzed. The DBS samples were analyzed for acylcarnitine and free amino acid profiles using quantitative electrospray tandem mass spectrometry. Metabolite ratios were calculated from relevant biochemical pathways that could identify relative changes in nutrient metabolism. The mean weight of Nile tilapia sampled from each lake showed weight variation among the lakes, fish from Lake Ziway were largest (178 g), followed by Gilgel Gibe reservoir (134 g) and Lake Langano (118 g). Fish from Gilgel Gibe showed significantly higher fat composition in all tissues (P < 0.05) except the liver in which no significant variation was observed. The source of fish affected the tissue fat composition. Marked differences were observed in Nile tilapia metabolic activity between the lakes. For instance, the lower body weight and condition of the fish in Lake Langano coincided with several metabolite ratios pointing to a low flow of glucogenic substrate to the citric acid cycle. The low propionyl to acetylcarnitine ratio (C3:C2) in Gilgel Gibe fish is indicating that more of the available acetyl CoA is not led into the citric acid cycle, but instead will be used for fat synthesis. The metabolic markers for lipogenesis and metabolic rate could explain the high-fat concentration in several parts of the body composition of fish from Gilgel Gibe. Our results show that nutrition-related blood metabolite ratios are useful to understand the underlying metabolic events leading to the habitat-dependent differences in the growth of Nile tilapia, and by extension, other species.
format	article
author	Tokuma Negisho Bayissa Michelle Geerardyn Donna Vanhauteghem Mulugeta Wakjira Geert Paul Jules Janssens
author_facet	Tokuma Negisho Bayissa Michelle Geerardyn Donna Vanhauteghem Mulugeta Wakjira Geert Paul Jules Janssens
author_sort	Tokuma Negisho Bayissa
title	Nutrient-related metabolite profiles explain differences in body composition and size in Nile tilapia (Oreochromis niloticus) from different lakes
title_short	Nutrient-related metabolite profiles explain differences in body composition and size in Nile tilapia (Oreochromis niloticus) from different lakes
title_full	Nutrient-related metabolite profiles explain differences in body composition and size in Nile tilapia (Oreochromis niloticus) from different lakes
title_fullStr	Nutrient-related metabolite profiles explain differences in body composition and size in Nile tilapia (Oreochromis niloticus) from different lakes
title_full_unstemmed	Nutrient-related metabolite profiles explain differences in body composition and size in Nile tilapia (Oreochromis niloticus) from different lakes
title_sort	nutrient-related metabolite profiles explain differences in body composition and size in nile tilapia (oreochromis niloticus) from different lakes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ac3af859f35b4bdf92d6ec4ebfc337cf
work_keys_str_mv	AT tokumanegishobayissa nutrientrelatedmetaboliteprofilesexplaindifferencesinbodycompositionandsizeinniletilapiaoreochromisniloticusfromdifferentlakes AT michellegeerardyn nutrientrelatedmetaboliteprofilesexplaindifferencesinbodycompositionandsizeinniletilapiaoreochromisniloticusfromdifferentlakes AT donnavanhauteghem nutrientrelatedmetaboliteprofilesexplaindifferencesinbodycompositionandsizeinniletilapiaoreochromisniloticusfromdifferentlakes AT mulugetawakjira nutrientrelatedmetaboliteprofilesexplaindifferencesinbodycompositionandsizeinniletilapiaoreochromisniloticusfromdifferentlakes AT geertpauljulesjanssens nutrientrelatedmetaboliteprofilesexplaindifferencesinbodycompositionandsizeinniletilapiaoreochromisniloticusfromdifferentlakes
_version_	1718383473020895232

Nutrient-related metabolite profiles explain differences in body composition and size in Nile tilapia (Oreochromis niloticus) from different lakes

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