Screening a peptide library by DSC and SAXD: comparison with the biological function of the parent proteins.

We have recently identified the membranotropic regions of the hepatitis C virus proteins E1, E2, core and p7 proteins by observing the effect of protein-derived peptide libraries on model membrane integrity. We have studied in this work the ability of selected sequences of these proteins to modulate...

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Autores principales:	Ana J Pérez-Berná, George Pabst, Peter Laggner, José Villalaín
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ac3d4081b7f44902bd4646ea22911838
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spelling	oai:doaj.org-article:ac3d4081b7f44902bd4646ea229118382021-11-25T06:17:29ZScreening a peptide library by DSC and SAXD: comparison with the biological function of the parent proteins.1932-620310.1371/journal.pone.0004356https://doaj.org/article/ac3d4081b7f44902bd4646ea229118382009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19194494/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203We have recently identified the membranotropic regions of the hepatitis C virus proteins E1, E2, core and p7 proteins by observing the effect of protein-derived peptide libraries on model membrane integrity. We have studied in this work the ability of selected sequences of these proteins to modulate the L(beta)-L(alpha) and L(alpha)-H(II) phospholipid phase transitions as well as check the viability of using both DSC and SAXD to screen a protein-derived peptide library. We demonstrate that it is feasible to screen a library of peptides corresponding to one or several proteins by both SAXD and DSC. This methodological combination should allow the identification of essential regions of membrane-interacting proteins which might be implicated in the molecular mechanism of membrane fusion and/or budding.Ana J Pérez-BernáGeorge PabstPeter LaggnerJosé VillalaínPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 2, p e4356 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ana J Pérez-Berná George Pabst Peter Laggner José Villalaín Screening a peptide library by DSC and SAXD: comparison with the biological function of the parent proteins.
description	We have recently identified the membranotropic regions of the hepatitis C virus proteins E1, E2, core and p7 proteins by observing the effect of protein-derived peptide libraries on model membrane integrity. We have studied in this work the ability of selected sequences of these proteins to modulate the L(beta)-L(alpha) and L(alpha)-H(II) phospholipid phase transitions as well as check the viability of using both DSC and SAXD to screen a protein-derived peptide library. We demonstrate that it is feasible to screen a library of peptides corresponding to one or several proteins by both SAXD and DSC. This methodological combination should allow the identification of essential regions of membrane-interacting proteins which might be implicated in the molecular mechanism of membrane fusion and/or budding.
format	article
author	Ana J Pérez-Berná George Pabst Peter Laggner José Villalaín
author_facet	Ana J Pérez-Berná George Pabst Peter Laggner José Villalaín
author_sort	Ana J Pérez-Berná
title	Screening a peptide library by DSC and SAXD: comparison with the biological function of the parent proteins.
title_short	Screening a peptide library by DSC and SAXD: comparison with the biological function of the parent proteins.
title_full	Screening a peptide library by DSC and SAXD: comparison with the biological function of the parent proteins.
title_fullStr	Screening a peptide library by DSC and SAXD: comparison with the biological function of the parent proteins.
title_full_unstemmed	Screening a peptide library by DSC and SAXD: comparison with the biological function of the parent proteins.
title_sort	screening a peptide library by dsc and saxd: comparison with the biological function of the parent proteins.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/ac3d4081b7f44902bd4646ea22911838
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Screening a peptide library by DSC and SAXD: comparison with the biological function of the parent proteins.

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