Multi-level kinetic model explaining diverse roles of isozymes in prokaryotes.

Current standard methods for kinetic and genomic modeling cannot provide deep insight into metabolic regulation. Here, we developed and evaluated a multi-scale kinetic modeling approach applicable to any prokaryote. Specifically, we highlight the primary metabolism of the cyanobacterium Synechococcu...

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Autores principales:	Jiri Jablonsky, Doreen Schwarz, Martin Hagemann
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ac44eb06276a4635b97e7e251fcbe855
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spelling	oai:doaj.org-article:ac44eb06276a4635b97e7e251fcbe8552021-11-25T06:04:37ZMulti-level kinetic model explaining diverse roles of isozymes in prokaryotes.1932-620310.1371/journal.pone.0105292https://doaj.org/article/ac44eb06276a4635b97e7e251fcbe8552014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25127487/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Current standard methods for kinetic and genomic modeling cannot provide deep insight into metabolic regulation. Here, we developed and evaluated a multi-scale kinetic modeling approach applicable to any prokaryote. Specifically, we highlight the primary metabolism of the cyanobacterium Synechococcus elongatus PCC 7942. The model bridges metabolic data sets from cells grown at different CO2 conditions by integrating transcriptomic data and isozymes. Identification of the regulatory roles of isozymes allowed the calculation and explanation of the absolute metabolic concentration of 3-phosphoglycerate. To demonstrate that this method can characterize any isozyme, we determined the function of two glycolytic glyceraldehyde-3-phosphate dehydrogenases: one co-regulates high concentrations of the 3-phosphoglycerate, the other shifts the bifurcation point in hexose regulation, and both improve biomass production. Moreover, the regulatory roles of multiple phosphoglycolate phosphatases were defined for varying (non-steady) CO2 conditions, suggesting their protective role against toxic photorespiratory intermediates.Jiri JablonskyDoreen SchwarzMartin HagemannPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e105292 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jiri Jablonsky Doreen Schwarz Martin Hagemann Multi-level kinetic model explaining diverse roles of isozymes in prokaryotes.
description	Current standard methods for kinetic and genomic modeling cannot provide deep insight into metabolic regulation. Here, we developed and evaluated a multi-scale kinetic modeling approach applicable to any prokaryote. Specifically, we highlight the primary metabolism of the cyanobacterium Synechococcus elongatus PCC 7942. The model bridges metabolic data sets from cells grown at different CO2 conditions by integrating transcriptomic data and isozymes. Identification of the regulatory roles of isozymes allowed the calculation and explanation of the absolute metabolic concentration of 3-phosphoglycerate. To demonstrate that this method can characterize any isozyme, we determined the function of two glycolytic glyceraldehyde-3-phosphate dehydrogenases: one co-regulates high concentrations of the 3-phosphoglycerate, the other shifts the bifurcation point in hexose regulation, and both improve biomass production. Moreover, the regulatory roles of multiple phosphoglycolate phosphatases were defined for varying (non-steady) CO2 conditions, suggesting their protective role against toxic photorespiratory intermediates.
format	article
author	Jiri Jablonsky Doreen Schwarz Martin Hagemann
author_facet	Jiri Jablonsky Doreen Schwarz Martin Hagemann
author_sort	Jiri Jablonsky
title	Multi-level kinetic model explaining diverse roles of isozymes in prokaryotes.
title_short	Multi-level kinetic model explaining diverse roles of isozymes in prokaryotes.
title_full	Multi-level kinetic model explaining diverse roles of isozymes in prokaryotes.
title_fullStr	Multi-level kinetic model explaining diverse roles of isozymes in prokaryotes.
title_full_unstemmed	Multi-level kinetic model explaining diverse roles of isozymes in prokaryotes.
title_sort	multi-level kinetic model explaining diverse roles of isozymes in prokaryotes.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/ac44eb06276a4635b97e7e251fcbe855
work_keys_str_mv	AT jirijablonsky multilevelkineticmodelexplainingdiverserolesofisozymesinprokaryotes AT doreenschwarz multilevelkineticmodelexplainingdiverserolesofisozymesinprokaryotes AT martinhagemann multilevelkineticmodelexplainingdiverserolesofisozymesinprokaryotes
_version_	1718414239958302720

Multi-level kinetic model explaining diverse roles of isozymes in prokaryotes.

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