Confocal laser scanning microscopy to estimate nanoparticles’ human skin penetration in vitro

Confocal laser scanning microscopy to estimate nanoparticles’ human skin penetration in vitro

Ying Zou,1,2,* Anna Celli,2,3,* Hanjiang Zhu,2,* Akram Elmahdy,2 Yachao Cao,2 Xiaoying Hui,2 Howard Maibach2 1Skin & Cosmetic Research Department, Shanghai Skin Disease Hospital, Shanghai, People’s Republic of China; 2Department of Dermatology, School of Medicine, University of Ca...

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Autores principales: Zou Y, Celli A, Zhu H, Elmahdy A, Cao Y, Hui X, Maibach H
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2017
Materias:
Nanoparticles
Skin penetration
Stratum corneum
Confocal laser scan microscopy
tape stripping
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/ac46b3c44cea4c3bad3d677c7dc0f4b1
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spelling oai:doaj.org-article:ac46b3c44cea4c3bad3d677c7dc0f4b12021-12-02T06:37:58ZConfocal laser scanning microscopy to estimate nanoparticles’ human skin penetration in vitro1178-2013https://doaj.org/article/ac46b3c44cea4c3bad3d677c7dc0f4b12017-10-01T00:00:00Zhttps://www.dovepress.com/confocal-laser-scanning-microscopy-to-estimate-nanoparticles-human-ski-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ying Zou,1,2,* Anna Celli,2,3,* Hanjiang Zhu,2,* Akram Elmahdy,2 Yachao Cao,2 Xiaoying Hui,2 Howard Maibach2 1Skin & Cosmetic Research Department, Shanghai Skin Disease Hospital, Shanghai, People’s Republic of China; 2Department of Dermatology, School of Medicine, University of California San Francisco, San Francisco, CA, USA; 3San Francisco Veterans Medical Center, San Francisco, CA, USA *These authors contributed equally to this work Objective: With rapid development of nanotechnology, there is increasing interest in nanoparticle (NP) application and its safety and efficacy on human skin. In this study, we utilized confocal laser scanning microscopy to estimate NP skin penetration.Methods: Three different-sized polystyrene NPs marked with red fluorescence were applied to human skin, and Calcium Green 5N was used as a counterstain. Dimethyl sulfoxide (DMSO) and ethanol were used as alternative vehicles for NPs. Tape stripping was utilized as a barrier-damaged skin model. Skin biopsies dosed with NPs were incubated at 4°C or 37°C for 24 hours and imaged using confocal laser scanning microscopy.Results: NPs were localized in the stratum corneum (SC) and hair follicles without penetrating the epidermis/dermis. Barrier alteration with tape stripping and change in incubation temperature did not induce deeper penetration. DMSO enhanced NP SC penetration but ethanol did not.Conclusion: Except with DMSO vehicle, these hydrolyzed polystyrene NPs did not penetrate intact or barrier-damaged human “viable” epidermis. For further clinical relevance, in vivo human skin studies and more sensitive analytic chemical methodology are suggested. Keywords: nanoparticles, skin penetration, stratum corneum, confocal laser scanning microscopy, tape strippingZou YCelli AZhu HElmahdy ACao YHui XMaibach HDove Medical PressarticleNanoparticlesSkin penetrationStratum corneumConfocal laser scan microscopytape strippingMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 8035-8041 (2017)
institution DOAJ
collection DOAJ
language EN
topic Nanoparticles
Skin penetration
Stratum corneum
Confocal laser scan microscopy
tape stripping
Medicine (General)
R5-920
spellingShingle Nanoparticles
Skin penetration
Stratum corneum
Confocal laser scan microscopy
tape stripping
Medicine (General)
R5-920
Zou Y
Celli A
Zhu H
Elmahdy A
Cao Y
Hui X
Maibach H
Confocal laser scanning microscopy to estimate nanoparticles’ human skin penetration in vitro
description Ying Zou,1,2,* Anna Celli,2,3,* Hanjiang Zhu,2,* Akram Elmahdy,2 Yachao Cao,2 Xiaoying Hui,2 Howard Maibach2 1Skin & Cosmetic Research Department, Shanghai Skin Disease Hospital, Shanghai, People’s Republic of China; 2Department of Dermatology, School of Medicine, University of California San Francisco, San Francisco, CA, USA; 3San Francisco Veterans Medical Center, San Francisco, CA, USA *These authors contributed equally to this work Objective: With rapid development of nanotechnology, there is increasing interest in nanoparticle (NP) application and its safety and efficacy on human skin. In this study, we utilized confocal laser scanning microscopy to estimate NP skin penetration.Methods: Three different-sized polystyrene NPs marked with red fluorescence were applied to human skin, and Calcium Green 5N was used as a counterstain. Dimethyl sulfoxide (DMSO) and ethanol were used as alternative vehicles for NPs. Tape stripping was utilized as a barrier-damaged skin model. Skin biopsies dosed with NPs were incubated at 4°C or 37°C for 24 hours and imaged using confocal laser scanning microscopy.Results: NPs were localized in the stratum corneum (SC) and hair follicles without penetrating the epidermis/dermis. Barrier alteration with tape stripping and change in incubation temperature did not induce deeper penetration. DMSO enhanced NP SC penetration but ethanol did not.Conclusion: Except with DMSO vehicle, these hydrolyzed polystyrene NPs did not penetrate intact or barrier-damaged human “viable” epidermis. For further clinical relevance, in vivo human skin studies and more sensitive analytic chemical methodology are suggested. Keywords: nanoparticles, skin penetration, stratum corneum, confocal laser scanning microscopy, tape stripping
format article
author Zou Y
Celli A
Zhu H
Elmahdy A
Cao Y
Hui X
Maibach H
author_facet Zou Y
Celli A
Zhu H
Elmahdy A
Cao Y
Hui X
Maibach H
author_sort Zou Y
title Confocal laser scanning microscopy to estimate nanoparticles’ human skin penetration in vitro
title_short Confocal laser scanning microscopy to estimate nanoparticles’ human skin penetration in vitro
title_full Confocal laser scanning microscopy to estimate nanoparticles’ human skin penetration in vitro
title_fullStr Confocal laser scanning microscopy to estimate nanoparticles’ human skin penetration in vitro
title_full_unstemmed Confocal laser scanning microscopy to estimate nanoparticles’ human skin penetration in vitro
title_sort confocal laser scanning microscopy to estimate nanoparticles’ human skin penetration in vitro
publisher Dove Medical Press
publishDate 2017
url https://doaj.org/article/ac46b3c44cea4c3bad3d677c7dc0f4b1
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