Fungi in dialysis water and dialysate: occurrence, susceptibility to antifungal agents and biofilm production capacity

The aim of this study was to investigate the occurrence of fungi in dialysis water and dialysate, in addition to evaluating the susceptibility to antifungals and the biofilm production capacity of isolated microorganisms. The samples were collected in three hemodialysis units in Bauru (Brazil), ever...

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Autores principales:	Laís Anversa, Bruna Rossini Lara, Caroline Demai Romani, Erika Kushikawa Saeki, Gabriel Antonio Nogueira Nascentes, Lucas Xavier Bonfietti, Márcia de Souza Carvalho Melhem, Luciana da Silva Ruiz, Carlos Henrique Camargo, Virgínia Bodelão Richini Pereira
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	antifungal susceptibility biofilm dialysate dialysis water fungi hemodialysis Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/ac4e2da7df494a04a73fb3c715a7d3c2
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spelling	oai:doaj.org-article:ac4e2da7df494a04a73fb3c715a7d3c22021-11-06T06:30:44ZFungi in dialysis water and dialysate: occurrence, susceptibility to antifungal agents and biofilm production capacity1477-89201996-782910.2166/wh.2021.204https://doaj.org/article/ac4e2da7df494a04a73fb3c715a7d3c22021-10-01T00:00:00Zhttp://jwh.iwaponline.com/content/19/5/724https://doaj.org/toc/1477-8920https://doaj.org/toc/1996-7829The aim of this study was to investigate the occurrence of fungi in dialysis water and dialysate, in addition to evaluating the susceptibility to antifungals and the biofilm production capacity of isolated microorganisms. The samples were collected in three hemodialysis units in Bauru (Brazil), every 15 days (July 2017–June 2018) at post-reverse osmosis, reuse, and dialysate points. The fungi were isolated by spread plate on Sabouraud dextrose agar. Filamentous fungi were phenotypically identified and yeasts were subjected to molecular evaluation of the ITS region. Susceptibility test to antifungals was carried out by the broth microdilution method and biofilm production capacity was evaluated in microtiter plates using crystal violet staining. Fungi were isolated in 52/216 (24.1%) samples, with an average count of 16.3 (10–40) CFU/mL. Overall, 61 microorganisms were identified, with 54 (88.5%) filamentous fungi and 7 (11.5%) yeasts. The main genera included were Penicillium, Cladosporium, Scedosporium, Rhinocladiella, Fusarium, and Emmonsia. Most isolates showed high values of minimum inhibitory concentration for 5-flucytosine and fluconazole and 35/45 (77.8%) isolates were classified as strong producers of biofilm. In order to increase the safety of the dialysis process, the adoption of control measures and monitoring of fungi in hemodialysis fluids is suggested. HIGHLIGHTS Fungi with pathogenic potential in hemodialysis fluids.; Fungi with low susceptibility to antifungals.; Fungi capable of producing biofilm.;Laís AnversaBruna Rossini LaraCaroline Demai RomaniErika Kushikawa SaekiGabriel Antonio Nogueira NascentesLucas Xavier BonfiettiMárcia de Souza Carvalho MelhemLuciana da Silva RuizCarlos Henrique CamargoVirgínia Bodelão Richini PereiraIWA Publishingarticleantifungal susceptibilitybiofilmdialysatedialysis waterfungihemodialysisPublic aspects of medicineRA1-1270ENJournal of Water and Health, Vol 19, Iss 5, Pp 724-735 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antifungal susceptibility biofilm dialysate dialysis water fungi hemodialysis Public aspects of medicine RA1-1270
spellingShingle	antifungal susceptibility biofilm dialysate dialysis water fungi hemodialysis Public aspects of medicine RA1-1270 Laís Anversa Bruna Rossini Lara Caroline Demai Romani Erika Kushikawa Saeki Gabriel Antonio Nogueira Nascentes Lucas Xavier Bonfietti Márcia de Souza Carvalho Melhem Luciana da Silva Ruiz Carlos Henrique Camargo Virgínia Bodelão Richini Pereira Fungi in dialysis water and dialysate: occurrence, susceptibility to antifungal agents and biofilm production capacity
description	The aim of this study was to investigate the occurrence of fungi in dialysis water and dialysate, in addition to evaluating the susceptibility to antifungals and the biofilm production capacity of isolated microorganisms. The samples were collected in three hemodialysis units in Bauru (Brazil), every 15 days (July 2017–June 2018) at post-reverse osmosis, reuse, and dialysate points. The fungi were isolated by spread plate on Sabouraud dextrose agar. Filamentous fungi were phenotypically identified and yeasts were subjected to molecular evaluation of the ITS region. Susceptibility test to antifungals was carried out by the broth microdilution method and biofilm production capacity was evaluated in microtiter plates using crystal violet staining. Fungi were isolated in 52/216 (24.1%) samples, with an average count of 16.3 (10–40) CFU/mL. Overall, 61 microorganisms were identified, with 54 (88.5%) filamentous fungi and 7 (11.5%) yeasts. The main genera included were Penicillium, Cladosporium, Scedosporium, Rhinocladiella, Fusarium, and Emmonsia. Most isolates showed high values of minimum inhibitory concentration for 5-flucytosine and fluconazole and 35/45 (77.8%) isolates were classified as strong producers of biofilm. In order to increase the safety of the dialysis process, the adoption of control measures and monitoring of fungi in hemodialysis fluids is suggested. HIGHLIGHTS Fungi with pathogenic potential in hemodialysis fluids.; Fungi with low susceptibility to antifungals.; Fungi capable of producing biofilm.;
format	article
author	Laís Anversa Bruna Rossini Lara Caroline Demai Romani Erika Kushikawa Saeki Gabriel Antonio Nogueira Nascentes Lucas Xavier Bonfietti Márcia de Souza Carvalho Melhem Luciana da Silva Ruiz Carlos Henrique Camargo Virgínia Bodelão Richini Pereira
author_facet	Laís Anversa Bruna Rossini Lara Caroline Demai Romani Erika Kushikawa Saeki Gabriel Antonio Nogueira Nascentes Lucas Xavier Bonfietti Márcia de Souza Carvalho Melhem Luciana da Silva Ruiz Carlos Henrique Camargo Virgínia Bodelão Richini Pereira
author_sort	Laís Anversa
title	Fungi in dialysis water and dialysate: occurrence, susceptibility to antifungal agents and biofilm production capacity
title_short	Fungi in dialysis water and dialysate: occurrence, susceptibility to antifungal agents and biofilm production capacity
title_full	Fungi in dialysis water and dialysate: occurrence, susceptibility to antifungal agents and biofilm production capacity
title_fullStr	Fungi in dialysis water and dialysate: occurrence, susceptibility to antifungal agents and biofilm production capacity
title_full_unstemmed	Fungi in dialysis water and dialysate: occurrence, susceptibility to antifungal agents and biofilm production capacity
title_sort	fungi in dialysis water and dialysate: occurrence, susceptibility to antifungal agents and biofilm production capacity
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/ac4e2da7df494a04a73fb3c715a7d3c2
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Fungi in dialysis water and dialysate: occurrence, susceptibility to antifungal agents and biofilm production capacity

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