Effects of Glutathione on Growth, Intestinal Antioxidant Capacity, Histology, Gene Expression, and Microbiota of Juvenile Triploid Oncorhynchus mykiss

This study aimed to demonstrate the effects of dietary glutathione (GSH) on growth, intestinal antioxidant capacity, histology, gene expression, and microbiota in juvenile triploid rainbow trout (Oncorhynchus mykiss). Different diets (G0-control, G100, G200, G400, and G800) containing graded levels...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chang’an Wang, Baohui Su, Shaoxia Lu, Shicheng Han, Haibo Jiang, Zhuang Li, Yang Liu, Hongbai Liu, Yuhong Yang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	triploid O. mykiss glutathione intestinal health growth microbiota Physiology QP1-981
Acceso en línea:	https://doaj.org/article/ac4eb506391241b884330b4b46f80196
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ac4eb506391241b884330b4b46f80196
record_format	dspace
spelling	oai:doaj.org-article:ac4eb506391241b884330b4b46f801962021-12-03T15:14:08ZEffects of Glutathione on Growth, Intestinal Antioxidant Capacity, Histology, Gene Expression, and Microbiota of Juvenile Triploid Oncorhynchus mykiss1664-042X10.3389/fphys.2021.784852https://doaj.org/article/ac4eb506391241b884330b4b46f801962021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphys.2021.784852/fullhttps://doaj.org/toc/1664-042XThis study aimed to demonstrate the effects of dietary glutathione (GSH) on growth, intestinal antioxidant capacity, histology, gene expression, and microbiota in juvenile triploid rainbow trout (Oncorhynchus mykiss). Different diets (G0-control, G100, G200, G400, and G800) containing graded levels of GSH (0, 100, 200, 400, and 800mgkg−1) were fed to triplicate groups of 30 fish (initial mean weight 4.12±0.04g) for 56days. G400 had significantly improved weight gain and feed conversion rate. Based on the broken-line regression analysis, the optimum dietary GSH level was 447.06mgkg−1. Catalase and superoxide dismutase activities were significantly higher in G200–G800. G200 had significantly lower malondialdehyde content. The height of the intestinal muscular layer in G400 was significantly higher than that of the control group. Intestinal PepT1 and SLC1A5 gene expression was significantly increased, and the highest was observed in G400. TNF-α, IL-1β, IL-2, and IL-8 expression were significantly decreased than that of G0. Next-generation sequencing of the 16S rDNA showed a significant difference in alpha diversity whereas no differences in beta diversity. On the genus level, LefSe analysis of indicator OTUs showed Ilumatobacter, Peptoniphilus, Limnobacter, Mizugakiibacter, Chelatococcus, Stella, Filimonas, and Streptosporangium were associated with the treatment diet, whereas Arcobacter, Ferrovibrio, Buchnera, Chitinophaga, Stenotrophobacter, Solimonadaceae, Polycyclovorans, Rhodococcus, Ramlibacter, and Azohydromonas were associated with the control diet. In summary, feeding juvenile triploid O. mykiss 200–800mgkg−1 GSH improved growth and intestinal health.Chang’an WangChang’an WangBaohui SuShaoxia LuShicheng HanHaibo JiangZhuang LiYang LiuHongbai LiuYuhong YangFrontiers Media S.A.articletriploid O. mykissglutathioneintestinal healthgrowthmicrobiotaPhysiologyQP1-981ENFrontiers in Physiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	triploid O. mykiss glutathione intestinal health growth microbiota Physiology QP1-981
spellingShingle	triploid O. mykiss glutathione intestinal health growth microbiota Physiology QP1-981 Chang’an Wang Chang’an Wang Baohui Su Shaoxia Lu Shicheng Han Haibo Jiang Zhuang Li Yang Liu Hongbai Liu Yuhong Yang Effects of Glutathione on Growth, Intestinal Antioxidant Capacity, Histology, Gene Expression, and Microbiota of Juvenile Triploid Oncorhynchus mykiss
description	This study aimed to demonstrate the effects of dietary glutathione (GSH) on growth, intestinal antioxidant capacity, histology, gene expression, and microbiota in juvenile triploid rainbow trout (Oncorhynchus mykiss). Different diets (G0-control, G100, G200, G400, and G800) containing graded levels of GSH (0, 100, 200, 400, and 800mgkg−1) were fed to triplicate groups of 30 fish (initial mean weight 4.12±0.04g) for 56days. G400 had significantly improved weight gain and feed conversion rate. Based on the broken-line regression analysis, the optimum dietary GSH level was 447.06mgkg−1. Catalase and superoxide dismutase activities were significantly higher in G200–G800. G200 had significantly lower malondialdehyde content. The height of the intestinal muscular layer in G400 was significantly higher than that of the control group. Intestinal PepT1 and SLC1A5 gene expression was significantly increased, and the highest was observed in G400. TNF-α, IL-1β, IL-2, and IL-8 expression were significantly decreased than that of G0. Next-generation sequencing of the 16S rDNA showed a significant difference in alpha diversity whereas no differences in beta diversity. On the genus level, LefSe analysis of indicator OTUs showed Ilumatobacter, Peptoniphilus, Limnobacter, Mizugakiibacter, Chelatococcus, Stella, Filimonas, and Streptosporangium were associated with the treatment diet, whereas Arcobacter, Ferrovibrio, Buchnera, Chitinophaga, Stenotrophobacter, Solimonadaceae, Polycyclovorans, Rhodococcus, Ramlibacter, and Azohydromonas were associated with the control diet. In summary, feeding juvenile triploid O. mykiss 200–800mgkg−1 GSH improved growth and intestinal health.
format	article
author	Chang’an Wang Chang’an Wang Baohui Su Shaoxia Lu Shicheng Han Haibo Jiang Zhuang Li Yang Liu Hongbai Liu Yuhong Yang
author_facet	Chang’an Wang Chang’an Wang Baohui Su Shaoxia Lu Shicheng Han Haibo Jiang Zhuang Li Yang Liu Hongbai Liu Yuhong Yang
author_sort	Chang’an Wang
title	Effects of Glutathione on Growth, Intestinal Antioxidant Capacity, Histology, Gene Expression, and Microbiota of Juvenile Triploid Oncorhynchus mykiss
title_short	Effects of Glutathione on Growth, Intestinal Antioxidant Capacity, Histology, Gene Expression, and Microbiota of Juvenile Triploid Oncorhynchus mykiss
title_full	Effects of Glutathione on Growth, Intestinal Antioxidant Capacity, Histology, Gene Expression, and Microbiota of Juvenile Triploid Oncorhynchus mykiss
title_fullStr	Effects of Glutathione on Growth, Intestinal Antioxidant Capacity, Histology, Gene Expression, and Microbiota of Juvenile Triploid Oncorhynchus mykiss
title_full_unstemmed	Effects of Glutathione on Growth, Intestinal Antioxidant Capacity, Histology, Gene Expression, and Microbiota of Juvenile Triploid Oncorhynchus mykiss
title_sort	effects of glutathione on growth, intestinal antioxidant capacity, histology, gene expression, and microbiota of juvenile triploid oncorhynchus mykiss
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/ac4eb506391241b884330b4b46f80196
work_keys_str_mv	AT changanwang effectsofglutathioneongrowthintestinalantioxidantcapacityhistologygeneexpressionandmicrobiotaofjuveniletriploidoncorhynchusmykiss AT changanwang effectsofglutathioneongrowthintestinalantioxidantcapacityhistologygeneexpressionandmicrobiotaofjuveniletriploidoncorhynchusmykiss AT baohuisu effectsofglutathioneongrowthintestinalantioxidantcapacityhistologygeneexpressionandmicrobiotaofjuveniletriploidoncorhynchusmykiss AT shaoxialu effectsofglutathioneongrowthintestinalantioxidantcapacityhistologygeneexpressionandmicrobiotaofjuveniletriploidoncorhynchusmykiss AT shichenghan effectsofglutathioneongrowthintestinalantioxidantcapacityhistologygeneexpressionandmicrobiotaofjuveniletriploidoncorhynchusmykiss AT haibojiang effectsofglutathioneongrowthintestinalantioxidantcapacityhistologygeneexpressionandmicrobiotaofjuveniletriploidoncorhynchusmykiss AT zhuangli effectsofglutathioneongrowthintestinalantioxidantcapacityhistologygeneexpressionandmicrobiotaofjuveniletriploidoncorhynchusmykiss AT yangliu effectsofglutathioneongrowthintestinalantioxidantcapacityhistologygeneexpressionandmicrobiotaofjuveniletriploidoncorhynchusmykiss AT hongbailiu effectsofglutathioneongrowthintestinalantioxidantcapacityhistologygeneexpressionandmicrobiotaofjuveniletriploidoncorhynchusmykiss AT yuhongyang effectsofglutathioneongrowthintestinalantioxidantcapacityhistologygeneexpressionandmicrobiotaofjuveniletriploidoncorhynchusmykiss
_version_	1718373168642523136

Effects of Glutathione on Growth, Intestinal Antioxidant Capacity, Histology, Gene Expression, and Microbiota of Juvenile Triploid Oncorhynchus mykiss

Ejemplares similares