Revealing the developmental dynamics in male strobilus transcriptome of Gnetum luofuense using nanopore sequencing technology

Abstract Gnetum is a pantropical distributed gymnosperm genus. As being dioecious, Gnetum species apply female and male strobili to attract and provide nutrition to insect pollinators. Due to its unique gross morphology, a Gnetum male strobilus receives much attention in previous taxonomic and evolu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chen Hou, Yuxin Tian, Yingli Wang, Huiming Lian, Dongcheng Liang, Shengqing Shi, Nan Deng, Boxiang He
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ac545e569b8a4749a05b0b5957d431d6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ac545e569b8a4749a05b0b5957d431d6
record_format	dspace
spelling	oai:doaj.org-article:ac545e569b8a4749a05b0b5957d431d62021-12-02T16:51:04ZRevealing the developmental dynamics in male strobilus transcriptome of Gnetum luofuense using nanopore sequencing technology10.1038/s41598-021-90082-02045-2322https://doaj.org/article/ac545e569b8a4749a05b0b5957d431d62021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90082-0https://doaj.org/toc/2045-2322Abstract Gnetum is a pantropical distributed gymnosperm genus. As being dioecious, Gnetum species apply female and male strobili to attract and provide nutrition to insect pollinators. Due to its unique gross morphology, a Gnetum male strobilus receives much attention in previous taxonomic and evolutionary studies. However, underlying molecular mechanisms that control male strobilus development and pollination adaptation have not been well studied. In the present study, nine full-length transcriptomes were sequenced from three developmental stages of the G. luofuense male strobili using Oxford Nanopore Technologies. In addition, weighted gene co-expression network analysis (WGCNA), and RT-qPCR analysis were performed. Our results show that a total of 3138 transcription factors and 466 long non-coding RNAs (lncRNAs) were identified, and differentially expressed lncRNAs and TFs reveal a dynamic pattern during the male strobilus development. Our results show that MADS-box and Aux/IAA TFs were differentially expressed at the three developmental stages, suggesting their important roles in the regulation of male strobilus development of G. luofuense. Results of WGCNA analysis and annotation of differentially expressed transcripts corroborate that the male strobilus development of G. luofuense is closely linked to plant hormone changes, photosynthesis, pollination drop secretion and reproductive organ defense. Our results provide a valuable resource for understanding the molecular mechanisms that drive organ evolution and pollination biology in Gnetum.Chen HouYuxin TianYingli WangHuiming LianDongcheng LiangShengqing ShiNan DengBoxiang HeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chen Hou Yuxin Tian Yingli Wang Huiming Lian Dongcheng Liang Shengqing Shi Nan Deng Boxiang He Revealing the developmental dynamics in male strobilus transcriptome of Gnetum luofuense using nanopore sequencing technology
description	Abstract Gnetum is a pantropical distributed gymnosperm genus. As being dioecious, Gnetum species apply female and male strobili to attract and provide nutrition to insect pollinators. Due to its unique gross morphology, a Gnetum male strobilus receives much attention in previous taxonomic and evolutionary studies. However, underlying molecular mechanisms that control male strobilus development and pollination adaptation have not been well studied. In the present study, nine full-length transcriptomes were sequenced from three developmental stages of the G. luofuense male strobili using Oxford Nanopore Technologies. In addition, weighted gene co-expression network analysis (WGCNA), and RT-qPCR analysis were performed. Our results show that a total of 3138 transcription factors and 466 long non-coding RNAs (lncRNAs) were identified, and differentially expressed lncRNAs and TFs reveal a dynamic pattern during the male strobilus development. Our results show that MADS-box and Aux/IAA TFs were differentially expressed at the three developmental stages, suggesting their important roles in the regulation of male strobilus development of G. luofuense. Results of WGCNA analysis and annotation of differentially expressed transcripts corroborate that the male strobilus development of G. luofuense is closely linked to plant hormone changes, photosynthesis, pollination drop secretion and reproductive organ defense. Our results provide a valuable resource for understanding the molecular mechanisms that drive organ evolution and pollination biology in Gnetum.
format	article
author	Chen Hou Yuxin Tian Yingli Wang Huiming Lian Dongcheng Liang Shengqing Shi Nan Deng Boxiang He
author_facet	Chen Hou Yuxin Tian Yingli Wang Huiming Lian Dongcheng Liang Shengqing Shi Nan Deng Boxiang He
author_sort	Chen Hou
title	Revealing the developmental dynamics in male strobilus transcriptome of Gnetum luofuense using nanopore sequencing technology
title_short	Revealing the developmental dynamics in male strobilus transcriptome of Gnetum luofuense using nanopore sequencing technology
title_full	Revealing the developmental dynamics in male strobilus transcriptome of Gnetum luofuense using nanopore sequencing technology
title_fullStr	Revealing the developmental dynamics in male strobilus transcriptome of Gnetum luofuense using nanopore sequencing technology
title_full_unstemmed	Revealing the developmental dynamics in male strobilus transcriptome of Gnetum luofuense using nanopore sequencing technology
title_sort	revealing the developmental dynamics in male strobilus transcriptome of gnetum luofuense using nanopore sequencing technology
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ac545e569b8a4749a05b0b5957d431d6
work_keys_str_mv	AT chenhou revealingthedevelopmentaldynamicsinmalestrobilustranscriptomeofgnetumluofuenseusingnanoporesequencingtechnology AT yuxintian revealingthedevelopmentaldynamicsinmalestrobilustranscriptomeofgnetumluofuenseusingnanoporesequencingtechnology AT yingliwang revealingthedevelopmentaldynamicsinmalestrobilustranscriptomeofgnetumluofuenseusingnanoporesequencingtechnology AT huiminglian revealingthedevelopmentaldynamicsinmalestrobilustranscriptomeofgnetumluofuenseusingnanoporesequencingtechnology AT dongchengliang revealingthedevelopmentaldynamicsinmalestrobilustranscriptomeofgnetumluofuenseusingnanoporesequencingtechnology AT shengqingshi revealingthedevelopmentaldynamicsinmalestrobilustranscriptomeofgnetumluofuenseusingnanoporesequencingtechnology AT nandeng revealingthedevelopmentaldynamicsinmalestrobilustranscriptomeofgnetumluofuenseusingnanoporesequencingtechnology AT boxianghe revealingthedevelopmentaldynamicsinmalestrobilustranscriptomeofgnetumluofuenseusingnanoporesequencingtechnology
_version_	1718382999933812736

Revealing the developmental dynamics in male strobilus transcriptome of Gnetum luofuense using nanopore sequencing technology

Ejemplares similares