A Novel Strategy to Engineer Pre-Vascularized Full-Length Dental Pulp-like Tissue Constructs

Abstract The requirement for immediate vascularization of engineered dental pulp poses a major hurdle towards successful implementation of pulp regeneration as an effective therapeutic strategy for root canal therapy, especially in adult teeth. Here, we demonstrate a novel strategy to engineer pre-v...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Avathamsa Athirasala, Fernanda Lins, Anthony Tahayeri, Monica Hinds, Anthony J. Smith, Christine Sedgley, Jack Ferracane, Luiz E. Bertassoni
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ac55dfd1867d4e76a388a1b269be79c7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ac55dfd1867d4e76a388a1b269be79c7
record_format	dspace
spelling	oai:doaj.org-article:ac55dfd1867d4e76a388a1b269be79c72021-12-02T12:31:53ZA Novel Strategy to Engineer Pre-Vascularized Full-Length Dental Pulp-like Tissue Constructs10.1038/s41598-017-02532-32045-2322https://doaj.org/article/ac55dfd1867d4e76a388a1b269be79c72017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02532-3https://doaj.org/toc/2045-2322Abstract The requirement for immediate vascularization of engineered dental pulp poses a major hurdle towards successful implementation of pulp regeneration as an effective therapeutic strategy for root canal therapy, especially in adult teeth. Here, we demonstrate a novel strategy to engineer pre-vascularized, cell-laden hydrogel pulp-like tissue constructs in full-length root canals for dental pulp regeneration. We utilized gelatin methacryloyl (GelMA) hydrogels with tunable physical and mechanical properties to determine the microenvironmental conditions (microstructure, degradation, swelling and elastic modulus) that enhanced viability, spreading and proliferation of encapsulated odontoblast-like cells (OD21), and the formation of endothelial monolayers by endothelial colony forming cells (ECFCs). GelMA hydrogels with higher polymer concentration (15% w/v) and stiffness enhanced OD21 cell viability, spreading and proliferation, as well as endothelial cell spreading and monolayer formation. We then fabricated pre-vascularized, full-length, dental pulp-like tissue constructs by dispensing OD21 cell-laden GelMA hydrogel prepolymer in root canals of extracted teeth and fabricating 500 µm channels throughout the root canals. ECFCs seeded into the microchannels successfully formed monolayers and underwent angiogenic sprouting within 7 days in culture. In summary, the proposed approach is a simple and effective strategy for engineering of pre-vascularized dental pulp constructs offering potentially beneficial translational outcomes.Avathamsa AthirasalaFernanda LinsAnthony TahayeriMonica HindsAnthony J. SmithChristine SedgleyJack FerracaneLuiz E. BertassoniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Avathamsa Athirasala Fernanda Lins Anthony Tahayeri Monica Hinds Anthony J. Smith Christine Sedgley Jack Ferracane Luiz E. Bertassoni A Novel Strategy to Engineer Pre-Vascularized Full-Length Dental Pulp-like Tissue Constructs
description	Abstract The requirement for immediate vascularization of engineered dental pulp poses a major hurdle towards successful implementation of pulp regeneration as an effective therapeutic strategy for root canal therapy, especially in adult teeth. Here, we demonstrate a novel strategy to engineer pre-vascularized, cell-laden hydrogel pulp-like tissue constructs in full-length root canals for dental pulp regeneration. We utilized gelatin methacryloyl (GelMA) hydrogels with tunable physical and mechanical properties to determine the microenvironmental conditions (microstructure, degradation, swelling and elastic modulus) that enhanced viability, spreading and proliferation of encapsulated odontoblast-like cells (OD21), and the formation of endothelial monolayers by endothelial colony forming cells (ECFCs). GelMA hydrogels with higher polymer concentration (15% w/v) and stiffness enhanced OD21 cell viability, spreading and proliferation, as well as endothelial cell spreading and monolayer formation. We then fabricated pre-vascularized, full-length, dental pulp-like tissue constructs by dispensing OD21 cell-laden GelMA hydrogel prepolymer in root canals of extracted teeth and fabricating 500 µm channels throughout the root canals. ECFCs seeded into the microchannels successfully formed monolayers and underwent angiogenic sprouting within 7 days in culture. In summary, the proposed approach is a simple and effective strategy for engineering of pre-vascularized dental pulp constructs offering potentially beneficial translational outcomes.
format	article
author	Avathamsa Athirasala Fernanda Lins Anthony Tahayeri Monica Hinds Anthony J. Smith Christine Sedgley Jack Ferracane Luiz E. Bertassoni
author_facet	Avathamsa Athirasala Fernanda Lins Anthony Tahayeri Monica Hinds Anthony J. Smith Christine Sedgley Jack Ferracane Luiz E. Bertassoni
author_sort	Avathamsa Athirasala
title	A Novel Strategy to Engineer Pre-Vascularized Full-Length Dental Pulp-like Tissue Constructs
title_short	A Novel Strategy to Engineer Pre-Vascularized Full-Length Dental Pulp-like Tissue Constructs
title_full	A Novel Strategy to Engineer Pre-Vascularized Full-Length Dental Pulp-like Tissue Constructs
title_fullStr	A Novel Strategy to Engineer Pre-Vascularized Full-Length Dental Pulp-like Tissue Constructs
title_full_unstemmed	A Novel Strategy to Engineer Pre-Vascularized Full-Length Dental Pulp-like Tissue Constructs
title_sort	novel strategy to engineer pre-vascularized full-length dental pulp-like tissue constructs
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ac55dfd1867d4e76a388a1b269be79c7
work_keys_str_mv	AT avathamsaathirasala anovelstrategytoengineerprevascularizedfulllengthdentalpulpliketissueconstructs AT fernandalins anovelstrategytoengineerprevascularizedfulllengthdentalpulpliketissueconstructs AT anthonytahayeri anovelstrategytoengineerprevascularizedfulllengthdentalpulpliketissueconstructs AT monicahinds anovelstrategytoengineerprevascularizedfulllengthdentalpulpliketissueconstructs AT anthonyjsmith anovelstrategytoengineerprevascularizedfulllengthdentalpulpliketissueconstructs AT christinesedgley anovelstrategytoengineerprevascularizedfulllengthdentalpulpliketissueconstructs AT jackferracane anovelstrategytoengineerprevascularizedfulllengthdentalpulpliketissueconstructs AT luizebertassoni anovelstrategytoengineerprevascularizedfulllengthdentalpulpliketissueconstructs AT avathamsaathirasala novelstrategytoengineerprevascularizedfulllengthdentalpulpliketissueconstructs AT fernandalins novelstrategytoengineerprevascularizedfulllengthdentalpulpliketissueconstructs AT anthonytahayeri novelstrategytoengineerprevascularizedfulllengthdentalpulpliketissueconstructs AT monicahinds novelstrategytoengineerprevascularizedfulllengthdentalpulpliketissueconstructs AT anthonyjsmith novelstrategytoengineerprevascularizedfulllengthdentalpulpliketissueconstructs AT christinesedgley novelstrategytoengineerprevascularizedfulllengthdentalpulpliketissueconstructs AT jackferracane novelstrategytoengineerprevascularizedfulllengthdentalpulpliketissueconstructs AT luizebertassoni novelstrategytoengineerprevascularizedfulllengthdentalpulpliketissueconstructs
_version_	1718394234541703168

A Novel Strategy to Engineer Pre-Vascularized Full-Length Dental Pulp-like Tissue Constructs

Ejemplares similares