Insights into dynamics of mobile genetic elements in hyperthermophilic environments from five new Thermococcus plasmids.

Mobilome of hyperthermophilic archaea dwelling in deep-sea hydrothermal vents is poorly characterized. To gain insight into genetic diversity and dynamics of mobile genetic elements in these environments we have sequenced five new plasmids from different Thermococcus strains that have been isolated...

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Autores principales:	Mart Krupovic, Mathieu Gonnet, Wajdi Ben Hania, Patrick Forterre, Gaël Erauso
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ac581a7d97bc45b18100fe5aee72652b
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spelling	oai:doaj.org-article:ac581a7d97bc45b18100fe5aee72652b2021-11-18T08:01:55ZInsights into dynamics of mobile genetic elements in hyperthermophilic environments from five new Thermococcus plasmids.1932-620310.1371/journal.pone.0049044https://doaj.org/article/ac581a7d97bc45b18100fe5aee72652b2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23326305/?tool=EBIhttps://doaj.org/toc/1932-6203Mobilome of hyperthermophilic archaea dwelling in deep-sea hydrothermal vents is poorly characterized. To gain insight into genetic diversity and dynamics of mobile genetic elements in these environments we have sequenced five new plasmids from different Thermococcus strains that have been isolated from geographically remote hydrothermal vents. The plasmids were ascribed to two subfamilies, pTN2-like and pEXT9a-like. Gene content and phylogenetic analyses illuminated a robust connection between pTN2-like plasmids and Pyrococcus abyssi virus 1 (PAV1), with roughly half of the viral genome being composed of genes that have homologues in plasmids. Unexpectedly, pEXT9a-like plasmids were found to be closely related to the previously sequenced plasmid pMETVU01 from Methanocaldococcus vulcanius M7. Our data suggests that the latter observation is most compatible with an unprecedented horizontal transfer of a pEXT9a-like plasmid from Thermococcales to Methanococcales. Gene content analysis revealed that thermococcal plasmids encode Hfq-like proteins and toxin-antitoxin (TA) systems of two different families, VapBC and RelBE. Notably, although abundant in archaeal genomes, to our knowledge, TA and hfq-like genes have not been previously found in archaeal plasmids or viruses. Finally, the plasmids described here might prove to be useful in developing new genetic tools for hyperthermophiles.Mart KrupovicMathieu GonnetWajdi Ben HaniaPatrick ForterreGaël ErausoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e49044 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mart Krupovic Mathieu Gonnet Wajdi Ben Hania Patrick Forterre Gaël Erauso Insights into dynamics of mobile genetic elements in hyperthermophilic environments from five new Thermococcus plasmids.
description	Mobilome of hyperthermophilic archaea dwelling in deep-sea hydrothermal vents is poorly characterized. To gain insight into genetic diversity and dynamics of mobile genetic elements in these environments we have sequenced five new plasmids from different Thermococcus strains that have been isolated from geographically remote hydrothermal vents. The plasmids were ascribed to two subfamilies, pTN2-like and pEXT9a-like. Gene content and phylogenetic analyses illuminated a robust connection between pTN2-like plasmids and Pyrococcus abyssi virus 1 (PAV1), with roughly half of the viral genome being composed of genes that have homologues in plasmids. Unexpectedly, pEXT9a-like plasmids were found to be closely related to the previously sequenced plasmid pMETVU01 from Methanocaldococcus vulcanius M7. Our data suggests that the latter observation is most compatible with an unprecedented horizontal transfer of a pEXT9a-like plasmid from Thermococcales to Methanococcales. Gene content analysis revealed that thermococcal plasmids encode Hfq-like proteins and toxin-antitoxin (TA) systems of two different families, VapBC and RelBE. Notably, although abundant in archaeal genomes, to our knowledge, TA and hfq-like genes have not been previously found in archaeal plasmids or viruses. Finally, the plasmids described here might prove to be useful in developing new genetic tools for hyperthermophiles.
format	article
author	Mart Krupovic Mathieu Gonnet Wajdi Ben Hania Patrick Forterre Gaël Erauso
author_facet	Mart Krupovic Mathieu Gonnet Wajdi Ben Hania Patrick Forterre Gaël Erauso
author_sort	Mart Krupovic
title	Insights into dynamics of mobile genetic elements in hyperthermophilic environments from five new Thermococcus plasmids.
title_short	Insights into dynamics of mobile genetic elements in hyperthermophilic environments from five new Thermococcus plasmids.
title_full	Insights into dynamics of mobile genetic elements in hyperthermophilic environments from five new Thermococcus plasmids.
title_fullStr	Insights into dynamics of mobile genetic elements in hyperthermophilic environments from five new Thermococcus plasmids.
title_full_unstemmed	Insights into dynamics of mobile genetic elements in hyperthermophilic environments from five new Thermococcus plasmids.
title_sort	insights into dynamics of mobile genetic elements in hyperthermophilic environments from five new thermococcus plasmids.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/ac581a7d97bc45b18100fe5aee72652b
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Insights into dynamics of mobile genetic elements in hyperthermophilic environments from five new Thermococcus plasmids.

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