The Ionic Selectivity of Lysenin Channels in Open and Sub-Conducting States

The electrochemical gradients established across cell membranes are paramount for the execution of biological functions. Besides ion channels, other transporters, such as exogenous pore-forming toxins, may present ionic selectivity upon reconstitution in natural and artificial lipid membranes and co...

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Autores principales:	Andrew Bogard, Pangaea W. Finn, Fulton McKinney, Ilinca M. Flacau, Aviana R. Smith, Rosey Whiting, Daniel Fologea
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	lysenin selectivity membrane voltage sub-conducting channels Chemical technology TP1-1185 Chemical engineering TP155-156
Acceso en línea:	https://doaj.org/article/ac5a60af50864157865740aefadd57b8
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spelling	oai:doaj.org-article:ac5a60af50864157865740aefadd57b82021-11-25T18:20:11ZThe Ionic Selectivity of Lysenin Channels in Open and Sub-Conducting States10.3390/membranes111108972077-0375https://doaj.org/article/ac5a60af50864157865740aefadd57b82021-11-01T00:00:00Zhttps://www.mdpi.com/2077-0375/11/11/897https://doaj.org/toc/2077-0375The electrochemical gradients established across cell membranes are paramount for the execution of biological functions. Besides ion channels, other transporters, such as exogenous pore-forming toxins, may present ionic selectivity upon reconstitution in natural and artificial lipid membranes and contribute to the electrochemical gradients. In this context, we utilized electrophysiology approaches to assess the ionic selectivity of the pore-forming toxin lysenin reconstituted in planar bilayer lipid membranes. The membrane voltages were determined from the reversal potentials recorded upon channel exposure to asymmetrical ionic conditions, and the permeability ratios were calculated from the fit with the Goldman–Hodgkin–Katz equation. Our work shows that lysenin channels are ion-selective and the determined permeability coefficients are cation and anion-species dependent. We also exploited the unique property of lysenin channels to transition to a stable sub-conducting state upon exposure to calcium ions and assessed their subsequent change in ionic selectivity. The observed loss of selectivity was implemented in an electrical model describing the dependency of reversal potentials on calcium concentration. In conclusion, our work demonstrates that this pore-forming toxin presents ionic selectivity but this is adjusted by the particular conduction state of the channels.Andrew BogardPangaea W. FinnFulton McKinneyIlinca M. FlacauAviana R. SmithRosey WhitingDaniel FologeaMDPI AGarticlelyseninselectivitymembrane voltagesub-conducting channelsChemical technologyTP1-1185Chemical engineeringTP155-156ENMembranes, Vol 11, Iss 897, p 897 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lysenin selectivity membrane voltage sub-conducting channels Chemical technology TP1-1185 Chemical engineering TP155-156
spellingShingle	lysenin selectivity membrane voltage sub-conducting channels Chemical technology TP1-1185 Chemical engineering TP155-156 Andrew Bogard Pangaea W. Finn Fulton McKinney Ilinca M. Flacau Aviana R. Smith Rosey Whiting Daniel Fologea The Ionic Selectivity of Lysenin Channels in Open and Sub-Conducting States
description	The electrochemical gradients established across cell membranes are paramount for the execution of biological functions. Besides ion channels, other transporters, such as exogenous pore-forming toxins, may present ionic selectivity upon reconstitution in natural and artificial lipid membranes and contribute to the electrochemical gradients. In this context, we utilized electrophysiology approaches to assess the ionic selectivity of the pore-forming toxin lysenin reconstituted in planar bilayer lipid membranes. The membrane voltages were determined from the reversal potentials recorded upon channel exposure to asymmetrical ionic conditions, and the permeability ratios were calculated from the fit with the Goldman–Hodgkin–Katz equation. Our work shows that lysenin channels are ion-selective and the determined permeability coefficients are cation and anion-species dependent. We also exploited the unique property of lysenin channels to transition to a stable sub-conducting state upon exposure to calcium ions and assessed their subsequent change in ionic selectivity. The observed loss of selectivity was implemented in an electrical model describing the dependency of reversal potentials on calcium concentration. In conclusion, our work demonstrates that this pore-forming toxin presents ionic selectivity but this is adjusted by the particular conduction state of the channels.
format	article
author	Andrew Bogard Pangaea W. Finn Fulton McKinney Ilinca M. Flacau Aviana R. Smith Rosey Whiting Daniel Fologea
author_facet	Andrew Bogard Pangaea W. Finn Fulton McKinney Ilinca M. Flacau Aviana R. Smith Rosey Whiting Daniel Fologea
author_sort	Andrew Bogard
title	The Ionic Selectivity of Lysenin Channels in Open and Sub-Conducting States
title_short	The Ionic Selectivity of Lysenin Channels in Open and Sub-Conducting States
title_full	The Ionic Selectivity of Lysenin Channels in Open and Sub-Conducting States
title_fullStr	The Ionic Selectivity of Lysenin Channels in Open and Sub-Conducting States
title_full_unstemmed	The Ionic Selectivity of Lysenin Channels in Open and Sub-Conducting States
title_sort	ionic selectivity of lysenin channels in open and sub-conducting states
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ac5a60af50864157865740aefadd57b8
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The Ionic Selectivity of Lysenin Channels in Open and Sub-Conducting States

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