Resolving dynamics and function of transient states in single enzyme molecules

Resolving dynamics and function of transient states in single enzyme molecules

T4 Lysozyme (T4L) is a model protein whose structure is extensively studied. Here the authors combine single-molecule and ensemble FRET measurements, FRET-positioning and screening and EPR spectroscopy to study the structural dynamics of T4L and describe its conformational landscape during the catal...

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Autores principales: Hugo Sanabria, Dmitro Rodnin, Katherina Hemmen, Thomas-Otavio Peulen, Suren Felekyan, Mark R. Fleissner, Mykola Dimura, Felix Koberling, Ralf Kühnemuth, Wayne Hubbell, Holger Gohlke, Claus A. M. Seidel
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Science
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Acceso en línea:https://doaj.org/article/ac5a713e761c4aa39bb9d667d450bea4
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