Genetic Variability of Acetolactate Synthase (ALS) Sequence in Centaurea cyanus Plants Resistant and Susceptible to Tribenuron-Methyl

Centaurea cyanus, belonging to the Asteraceae family, is an arable weed species encountered mainly in fields with cereals, sugar beet, and maize. The high genetic variability of C. cyanus has been recently reported; however, little is known about its sequence va...

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Autores principales:	Barbara Wrzesińska, Tadeusz Praczyk
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Centaurea cyanus</i> herbicide resistance ALS sequence acetolactate synthase cornflower genetic variability Agriculture S
Acceso en línea:	https://doaj.org/article/ac5e9d0912924af080303e9d5db7ca9e
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id	oai:doaj.org-article:ac5e9d0912924af080303e9d5db7ca9e
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spelling	oai:doaj.org-article:ac5e9d0912924af080303e9d5db7ca9e2021-11-25T16:11:13ZGenetic Variability of Acetolactate Synthase (ALS) Sequence in <i>Centaurea cyanus</i> Plants Resistant and Susceptible to Tribenuron-Methyl10.3390/agronomy111123112073-4395https://doaj.org/article/ac5e9d0912924af080303e9d5db7ca9e2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4395/11/11/2311https://doaj.org/toc/2073-4395<i>Centaurea cyanus</i>, belonging to the Asteraceae family, is an arable weed species encountered mainly in fields with cereals, sugar beet, and maize. The high genetic variability of <i>C. cyanus</i> has been recently reported; however, little is known about its sequence variability in the context of its herbicide resistance. <i>C. cyanus</i> resistance was found mainly against acetolactate synthase (ALS) inhibitors, but no ALS sequence information concerning the herbicide resistance mechanism has been published yet. The aim of this study was to determine the <i>ALS</i> sequences for biotypes susceptible and resistant to tribenuron-methyl in order to identify mutations that may be associated with the resistance emergence. DNA isolation from susceptible and resistant plants was followed by PCR amplification and <i>ALS</i> sequencing. As a result, different lengths of DNA products were obtained. Moreover, both nucleotide and amino acid sequence analysis revealed high sequence variability within one plant as well as between plants from the same biotype. In a few resistant plants, four changes in the amino acid sequence were identified in comparison to those in the susceptible ones. However, these preliminary studies require further investigation toward confirming the significance of these mutations in herbicide resistance development. This study provides preliminary information contributing to the research on the <i>C. cyanus</i> target-site resistance mechanism.Barbara WrzesińskaTadeusz PraczykMDPI AGarticle<i>Centaurea cyanus</i>herbicide resistanceALS sequenceacetolactate synthasecornflowergenetic variabilityAgricultureSENAgronomy, Vol 11, Iss 2311, p 2311 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Centaurea cyanus</i> herbicide resistance ALS sequence acetolactate synthase cornflower genetic variability Agriculture S
spellingShingle	<i>Centaurea cyanus</i> herbicide resistance ALS sequence acetolactate synthase cornflower genetic variability Agriculture S Barbara Wrzesińska Tadeusz Praczyk Genetic Variability of Acetolactate Synthase (ALS) Sequence in <i>Centaurea cyanus</i> Plants Resistant and Susceptible to Tribenuron-Methyl
description	<i>Centaurea cyanus</i>, belonging to the Asteraceae family, is an arable weed species encountered mainly in fields with cereals, sugar beet, and maize. The high genetic variability of <i>C. cyanus</i> has been recently reported; however, little is known about its sequence variability in the context of its herbicide resistance. <i>C. cyanus</i> resistance was found mainly against acetolactate synthase (ALS) inhibitors, but no ALS sequence information concerning the herbicide resistance mechanism has been published yet. The aim of this study was to determine the <i>ALS</i> sequences for biotypes susceptible and resistant to tribenuron-methyl in order to identify mutations that may be associated with the resistance emergence. DNA isolation from susceptible and resistant plants was followed by PCR amplification and <i>ALS</i> sequencing. As a result, different lengths of DNA products were obtained. Moreover, both nucleotide and amino acid sequence analysis revealed high sequence variability within one plant as well as between plants from the same biotype. In a few resistant plants, four changes in the amino acid sequence were identified in comparison to those in the susceptible ones. However, these preliminary studies require further investigation toward confirming the significance of these mutations in herbicide resistance development. This study provides preliminary information contributing to the research on the <i>C. cyanus</i> target-site resistance mechanism.
format	article
author	Barbara Wrzesińska Tadeusz Praczyk
author_facet	Barbara Wrzesińska Tadeusz Praczyk
author_sort	Barbara Wrzesińska
title	Genetic Variability of Acetolactate Synthase (ALS) Sequence in <i>Centaurea cyanus</i> Plants Resistant and Susceptible to Tribenuron-Methyl
title_short	Genetic Variability of Acetolactate Synthase (ALS) Sequence in <i>Centaurea cyanus</i> Plants Resistant and Susceptible to Tribenuron-Methyl
title_full	Genetic Variability of Acetolactate Synthase (ALS) Sequence in <i>Centaurea cyanus</i> Plants Resistant and Susceptible to Tribenuron-Methyl
title_fullStr	Genetic Variability of Acetolactate Synthase (ALS) Sequence in <i>Centaurea cyanus</i> Plants Resistant and Susceptible to Tribenuron-Methyl
title_full_unstemmed	Genetic Variability of Acetolactate Synthase (ALS) Sequence in <i>Centaurea cyanus</i> Plants Resistant and Susceptible to Tribenuron-Methyl
title_sort	genetic variability of acetolactate synthase (als) sequence in <i>centaurea cyanus</i> plants resistant and susceptible to tribenuron-methyl
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ac5e9d0912924af080303e9d5db7ca9e
work_keys_str_mv	AT barbarawrzesinska geneticvariabilityofacetolactatesynthasealssequenceinicentaureacyanusiplantsresistantandsusceptibletotribenuronmethyl AT tadeuszpraczyk geneticvariabilityofacetolactatesynthasealssequenceinicentaureacyanusiplantsresistantandsusceptibletotribenuronmethyl
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Genetic Variability of Acetolactate Synthase (ALS) Sequence in <i>Centaurea cyanus</i> Plants Resistant and Susceptible to Tribenuron-Methyl

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