Nitric-oxide-driven oxygen release in anoxic Pseudomonas aeruginosa

Summary: Denitrification supports anoxic growth of Pseudomonas aeruginosa in infections. Moreover, denitrification may provide oxygen (O2) resulting from dismutation of the denitrification intermediate nitric oxide (NO) as seen in Methylomirabilis oxyfera. To examine the prevalence of NO dismutation...

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Autores principales:	Mads Lichtenberg, Laura Line, Verena Schrameyer, Tim Holm Jakobsen, Morten Levin Rybtke, Masanori Toyofuku, Nobuhiko Nomura, Mette Kolpen, Tim Tolker-Nielsen, Michael Kühl, Thomas Bjarnsholt, Peter Østrup Jensen
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Biological sciences Microbiology Microbial physiology Science Q
Acceso en línea:	https://doaj.org/article/ac5f71a751ac424c8bcb69645c76dde7
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spelling	oai:doaj.org-article:ac5f71a751ac424c8bcb69645c76dde72021-11-22T04:28:34ZNitric-oxide-driven oxygen release in anoxic Pseudomonas aeruginosa2589-004210.1016/j.isci.2021.103404https://doaj.org/article/ac5f71a751ac424c8bcb69645c76dde72021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2589004221013754https://doaj.org/toc/2589-0042Summary: Denitrification supports anoxic growth of Pseudomonas aeruginosa in infections. Moreover, denitrification may provide oxygen (O2) resulting from dismutation of the denitrification intermediate nitric oxide (NO) as seen in Methylomirabilis oxyfera. To examine the prevalence of NO dismutation we studied O2 release by P. aeruginosa in airtight vials. P. aeruginosa rapidly depleted O2 but NO supplementation generated peaks of O2 at the onset of anoxia, and we demonstrate a direct role of NO in the O2 release. However, we were not able to detect genetic evidence for putative NO dismutases.The supply of endogenous O2 at the onset of anoxia could play an adaptive role when P. aeruginosa enters anaerobiosis. Furthermore, O2 generation by NO dismutation may be more widespread than indicated by the reports on the distribution of homologues genes. In general, NO dismutation may allow removal of nitrate by denitrification without release of the very potent greenhouse gas, nitrous oxide.Mads LichtenbergLaura LineVerena SchrameyerTim Holm JakobsenMorten Levin RybtkeMasanori ToyofukuNobuhiko NomuraMette KolpenTim Tolker-NielsenMichael KühlThomas BjarnsholtPeter Østrup JensenElsevierarticleBiological sciencesMicrobiologyMicrobial physiologyScienceQENiScience, Vol 24, Iss 12, Pp 103404- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biological sciences Microbiology Microbial physiology Science Q
spellingShingle	Biological sciences Microbiology Microbial physiology Science Q Mads Lichtenberg Laura Line Verena Schrameyer Tim Holm Jakobsen Morten Levin Rybtke Masanori Toyofuku Nobuhiko Nomura Mette Kolpen Tim Tolker-Nielsen Michael Kühl Thomas Bjarnsholt Peter Østrup Jensen Nitric-oxide-driven oxygen release in anoxic Pseudomonas aeruginosa
description	Summary: Denitrification supports anoxic growth of Pseudomonas aeruginosa in infections. Moreover, denitrification may provide oxygen (O2) resulting from dismutation of the denitrification intermediate nitric oxide (NO) as seen in Methylomirabilis oxyfera. To examine the prevalence of NO dismutation we studied O2 release by P. aeruginosa in airtight vials. P. aeruginosa rapidly depleted O2 but NO supplementation generated peaks of O2 at the onset of anoxia, and we demonstrate a direct role of NO in the O2 release. However, we were not able to detect genetic evidence for putative NO dismutases.The supply of endogenous O2 at the onset of anoxia could play an adaptive role when P. aeruginosa enters anaerobiosis. Furthermore, O2 generation by NO dismutation may be more widespread than indicated by the reports on the distribution of homologues genes. In general, NO dismutation may allow removal of nitrate by denitrification without release of the very potent greenhouse gas, nitrous oxide.
format	article
author	Mads Lichtenberg Laura Line Verena Schrameyer Tim Holm Jakobsen Morten Levin Rybtke Masanori Toyofuku Nobuhiko Nomura Mette Kolpen Tim Tolker-Nielsen Michael Kühl Thomas Bjarnsholt Peter Østrup Jensen
author_facet	Mads Lichtenberg Laura Line Verena Schrameyer Tim Holm Jakobsen Morten Levin Rybtke Masanori Toyofuku Nobuhiko Nomura Mette Kolpen Tim Tolker-Nielsen Michael Kühl Thomas Bjarnsholt Peter Østrup Jensen
author_sort	Mads Lichtenberg
title	Nitric-oxide-driven oxygen release in anoxic Pseudomonas aeruginosa
title_short	Nitric-oxide-driven oxygen release in anoxic Pseudomonas aeruginosa
title_full	Nitric-oxide-driven oxygen release in anoxic Pseudomonas aeruginosa
title_fullStr	Nitric-oxide-driven oxygen release in anoxic Pseudomonas aeruginosa
title_full_unstemmed	Nitric-oxide-driven oxygen release in anoxic Pseudomonas aeruginosa
title_sort	nitric-oxide-driven oxygen release in anoxic pseudomonas aeruginosa
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/ac5f71a751ac424c8bcb69645c76dde7
work_keys_str_mv	AT madslichtenberg nitricoxidedrivenoxygenreleaseinanoxicpseudomonasaeruginosa AT lauraline nitricoxidedrivenoxygenreleaseinanoxicpseudomonasaeruginosa AT verenaschrameyer nitricoxidedrivenoxygenreleaseinanoxicpseudomonasaeruginosa AT timholmjakobsen nitricoxidedrivenoxygenreleaseinanoxicpseudomonasaeruginosa AT mortenlevinrybtke nitricoxidedrivenoxygenreleaseinanoxicpseudomonasaeruginosa AT masanoritoyofuku nitricoxidedrivenoxygenreleaseinanoxicpseudomonasaeruginosa AT nobuhikonomura nitricoxidedrivenoxygenreleaseinanoxicpseudomonasaeruginosa AT mettekolpen nitricoxidedrivenoxygenreleaseinanoxicpseudomonasaeruginosa AT timtolkernielsen nitricoxidedrivenoxygenreleaseinanoxicpseudomonasaeruginosa AT michaelkuhl nitricoxidedrivenoxygenreleaseinanoxicpseudomonasaeruginosa AT thomasbjarnsholt nitricoxidedrivenoxygenreleaseinanoxicpseudomonasaeruginosa AT peterøstrupjensen nitricoxidedrivenoxygenreleaseinanoxicpseudomonasaeruginosa
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Nitric-oxide-driven oxygen release in anoxic Pseudomonas aeruginosa

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