Microstructural Tissue Changes in a Rat Model of Mild Traumatic Brain Injury

Our study investigates the potential of diffusion MRI (dMRI), including diffusion tensor imaging (DTI), fixel-based analysis (FBA) and neurite orientation dispersion and density imaging (NODDI), to detect microstructural tissue abnormalities in rats after mild traumatic brain injury (mTBI). The brai...

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Autores principales:	Karthik Chary, Omar Narvaez, Raimo A. Salo, Isabel San Martín Molina, Jussi Tohka, Manisha Aggarwal, Olli Gröhn, Alejandra Sierra
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	traumatic brain injury fixel-based analysis diffusion tensor imaging axonal damage gliosis histology Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/ac63af19e9f04287a49b5031ab1eb3a3
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spelling	oai:doaj.org-article:ac63af19e9f04287a49b5031ab1eb3a32021-12-01T07:54:07ZMicrostructural Tissue Changes in a Rat Model of Mild Traumatic Brain Injury1662-453X10.3389/fnins.2021.746214https://doaj.org/article/ac63af19e9f04287a49b5031ab1eb3a32021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fnins.2021.746214/fullhttps://doaj.org/toc/1662-453XOur study investigates the potential of diffusion MRI (dMRI), including diffusion tensor imaging (DTI), fixel-based analysis (FBA) and neurite orientation dispersion and density imaging (NODDI), to detect microstructural tissue abnormalities in rats after mild traumatic brain injury (mTBI). The brains of sham-operated and mTBI rats 35 days after lateral fluid percussion injury were imaged ex vivo in a 11.7-T scanner. Voxel-based analyses of DTI-, fixel- and NODDI-based metrics detected extensive tissue changes in directly affected brain areas close to the primary injury, and more importantly, also in distal areas connected to primary injury and indirectly affected by the secondary injury mechanisms. Histology revealed ongoing axonal abnormalities and inflammation, 35 days after the injury, in the brain areas highlighted in the group analyses. Fractional anisotropy (FA), fiber density (FD) and fiber density and fiber bundle cross-section (FDC) showed similar pattern of significant areas throughout the brain; however, FA showed more significant voxels in gray matter areas, while FD and FDC in white matter areas, and orientation dispersion index (ODI) in areas most damage based on histology. Region-of-interest (ROI)-based analyses on dMRI maps and histology in selected brain regions revealed that the changes in MRI parameters could be attributed to both alterations in myelinated fiber bundles and increased cellularity. This study demonstrates that the combination of dMRI methods can provide a more complete insight into the microstructural alterations in white and gray matter after mTBI, which may aid diagnosis and prognosis following a mild brain injury.Karthik CharyOmar NarvaezRaimo A. SaloIsabel San Martín MolinaJussi TohkaManisha AggarwalOlli GröhnAlejandra SierraFrontiers Media S.A.articletraumatic brain injuryfixel-based analysisdiffusion tensor imagingaxonal damagegliosishistologyNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENFrontiers in Neuroscience, Vol 15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	traumatic brain injury fixel-based analysis diffusion tensor imaging axonal damage gliosis histology Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	traumatic brain injury fixel-based analysis diffusion tensor imaging axonal damage gliosis histology Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Karthik Chary Omar Narvaez Raimo A. Salo Isabel San Martín Molina Jussi Tohka Manisha Aggarwal Olli Gröhn Alejandra Sierra Microstructural Tissue Changes in a Rat Model of Mild Traumatic Brain Injury
description	Our study investigates the potential of diffusion MRI (dMRI), including diffusion tensor imaging (DTI), fixel-based analysis (FBA) and neurite orientation dispersion and density imaging (NODDI), to detect microstructural tissue abnormalities in rats after mild traumatic brain injury (mTBI). The brains of sham-operated and mTBI rats 35 days after lateral fluid percussion injury were imaged ex vivo in a 11.7-T scanner. Voxel-based analyses of DTI-, fixel- and NODDI-based metrics detected extensive tissue changes in directly affected brain areas close to the primary injury, and more importantly, also in distal areas connected to primary injury and indirectly affected by the secondary injury mechanisms. Histology revealed ongoing axonal abnormalities and inflammation, 35 days after the injury, in the brain areas highlighted in the group analyses. Fractional anisotropy (FA), fiber density (FD) and fiber density and fiber bundle cross-section (FDC) showed similar pattern of significant areas throughout the brain; however, FA showed more significant voxels in gray matter areas, while FD and FDC in white matter areas, and orientation dispersion index (ODI) in areas most damage based on histology. Region-of-interest (ROI)-based analyses on dMRI maps and histology in selected brain regions revealed that the changes in MRI parameters could be attributed to both alterations in myelinated fiber bundles and increased cellularity. This study demonstrates that the combination of dMRI methods can provide a more complete insight into the microstructural alterations in white and gray matter after mTBI, which may aid diagnosis and prognosis following a mild brain injury.
format	article
author	Karthik Chary Omar Narvaez Raimo A. Salo Isabel San Martín Molina Jussi Tohka Manisha Aggarwal Olli Gröhn Alejandra Sierra
author_facet	Karthik Chary Omar Narvaez Raimo A. Salo Isabel San Martín Molina Jussi Tohka Manisha Aggarwal Olli Gröhn Alejandra Sierra
author_sort	Karthik Chary
title	Microstructural Tissue Changes in a Rat Model of Mild Traumatic Brain Injury
title_short	Microstructural Tissue Changes in a Rat Model of Mild Traumatic Brain Injury
title_full	Microstructural Tissue Changes in a Rat Model of Mild Traumatic Brain Injury
title_fullStr	Microstructural Tissue Changes in a Rat Model of Mild Traumatic Brain Injury
title_full_unstemmed	Microstructural Tissue Changes in a Rat Model of Mild Traumatic Brain Injury
title_sort	microstructural tissue changes in a rat model of mild traumatic brain injury
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/ac63af19e9f04287a49b5031ab1eb3a3
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_version_	1718405433069142016

Microstructural Tissue Changes in a Rat Model of Mild Traumatic Brain Injury

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