Sensitive genotyping of mutations in the EGFR gene from NSCLC patients using PCR-GoldMag lateral flow device

Abstract Epidermal growth factor receptor (EGFR) mutations predict better outcomes with EGFR tyrosine kinase inhibitors in patients with non-small cell lung cancer (NSCLC). Most common activating mutations include in-frame deletion in exon 19 and L858R substitution in exon 21, which account for >...

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Autores principales:	Xian-Ying Li, Chao Zhang, Qin-Lu Zhang, Juan-Li Zhu, Qian Liu, Ming-Wei Chen, Xue-Min Yang, Wen-Li Hui, Ya-Li Cui
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ac6a5c9980654cd8ab230d3fecbc3679
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spelling	oai:doaj.org-article:ac6a5c9980654cd8ab230d3fecbc36792021-12-02T16:06:10ZSensitive genotyping of mutations in the EGFR gene from NSCLC patients using PCR-GoldMag lateral flow device10.1038/s41598-017-08210-82045-2322https://doaj.org/article/ac6a5c9980654cd8ab230d3fecbc36792017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08210-8https://doaj.org/toc/2045-2322Abstract Epidermal growth factor receptor (EGFR) mutations predict better outcomes with EGFR tyrosine kinase inhibitors in patients with non-small cell lung cancer (NSCLC). Most common activating mutations include in-frame deletion in exon 19 and L858R substitution in exon 21, which account for >90% of all EGFR mutations in NSCLC. In this study, a PCR-GoldMag lateral flow assay (PCR-GoldMag LFA) was developed for the visual detection of delE746-A750 and L858R of EGFR mutations. Forty formalin-fixed paraffin-embedded (FFPE) tissue samples of NSCLC patients were analyzed using PCR-GoldMag LFA system and verified by direct sequencing and TaqMan-PCR detection methods. Results showed that EGFR mutations were detected in 34 cases among the 40 samples (85%) by PCR-GoldMag LFA method. Among the 34 cases, 5 cases were simultaneously detected with delE746-A750 in exon 19 and L858R mutation in exon 21. Compared with sequencing, only 4 samples were detected as delE746-A750, which revealed higher sensitivity of PCR-GoldMag LFA detection method than direct sequencing. TaqMan-PCR method verified the L858R mutation and was in 100% agreement with our method. These results indicated that our method has obvious advantages to analyze clinical samples and offers a more sensitive alternative to direct sequencing for the detection of EGFR mutations.Xian-Ying LiChao ZhangQin-Lu ZhangJuan-Li ZhuQian LiuMing-Wei ChenXue-Min YangWen-Li HuiYa-Li CuiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xian-Ying Li Chao Zhang Qin-Lu Zhang Juan-Li Zhu Qian Liu Ming-Wei Chen Xue-Min Yang Wen-Li Hui Ya-Li Cui Sensitive genotyping of mutations in the EGFR gene from NSCLC patients using PCR-GoldMag lateral flow device
description	Abstract Epidermal growth factor receptor (EGFR) mutations predict better outcomes with EGFR tyrosine kinase inhibitors in patients with non-small cell lung cancer (NSCLC). Most common activating mutations include in-frame deletion in exon 19 and L858R substitution in exon 21, which account for >90% of all EGFR mutations in NSCLC. In this study, a PCR-GoldMag lateral flow assay (PCR-GoldMag LFA) was developed for the visual detection of delE746-A750 and L858R of EGFR mutations. Forty formalin-fixed paraffin-embedded (FFPE) tissue samples of NSCLC patients were analyzed using PCR-GoldMag LFA system and verified by direct sequencing and TaqMan-PCR detection methods. Results showed that EGFR mutations were detected in 34 cases among the 40 samples (85%) by PCR-GoldMag LFA method. Among the 34 cases, 5 cases were simultaneously detected with delE746-A750 in exon 19 and L858R mutation in exon 21. Compared with sequencing, only 4 samples were detected as delE746-A750, which revealed higher sensitivity of PCR-GoldMag LFA detection method than direct sequencing. TaqMan-PCR method verified the L858R mutation and was in 100% agreement with our method. These results indicated that our method has obvious advantages to analyze clinical samples and offers a more sensitive alternative to direct sequencing for the detection of EGFR mutations.
format	article
author	Xian-Ying Li Chao Zhang Qin-Lu Zhang Juan-Li Zhu Qian Liu Ming-Wei Chen Xue-Min Yang Wen-Li Hui Ya-Li Cui
author_facet	Xian-Ying Li Chao Zhang Qin-Lu Zhang Juan-Li Zhu Qian Liu Ming-Wei Chen Xue-Min Yang Wen-Li Hui Ya-Li Cui
author_sort	Xian-Ying Li
title	Sensitive genotyping of mutations in the EGFR gene from NSCLC patients using PCR-GoldMag lateral flow device
title_short	Sensitive genotyping of mutations in the EGFR gene from NSCLC patients using PCR-GoldMag lateral flow device
title_full	Sensitive genotyping of mutations in the EGFR gene from NSCLC patients using PCR-GoldMag lateral flow device
title_fullStr	Sensitive genotyping of mutations in the EGFR gene from NSCLC patients using PCR-GoldMag lateral flow device
title_full_unstemmed	Sensitive genotyping of mutations in the EGFR gene from NSCLC patients using PCR-GoldMag lateral flow device
title_sort	sensitive genotyping of mutations in the egfr gene from nsclc patients using pcr-goldmag lateral flow device
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ac6a5c9980654cd8ab230d3fecbc3679
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Sensitive genotyping of mutations in the EGFR gene from NSCLC patients using PCR-GoldMag lateral flow device

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