Transcriptional Correlates of Chronic Alcohol Neuroadaptation in Drosophila Larvae

When presented with the choice, Drosophila melanogaster females will often prefer to lay eggs on food containing a significant amount of alcohol. While, in some cases, this behavioral decision can provide a survival advantage to the developing larvae, it can also lead to developmental and cognitive...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Amanda Anqueira-González, Jenny P. Acevedo-Gonzalez, Airined Montes-Mercado, Claudia Irizarry-Hernández, Nicolás L. Fuenzalida-Uribe, Alfredo Ghezzi
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	neuroadaptation alcohol use disorder Drosophila melanogaster cognition differential gene expression RNA-seq Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/ac75cea8cf3f44208c1cf336b69acbfb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ac75cea8cf3f44208c1cf336b69acbfb
record_format	dspace
spelling	oai:doaj.org-article:ac75cea8cf3f44208c1cf336b69acbfb2021-11-04T06:07:01ZTranscriptional Correlates of Chronic Alcohol Neuroadaptation in Drosophila Larvae1662-515310.3389/fnbeh.2021.768694https://doaj.org/article/ac75cea8cf3f44208c1cf336b69acbfb2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fnbeh.2021.768694/fullhttps://doaj.org/toc/1662-5153When presented with the choice, Drosophila melanogaster females will often prefer to lay eggs on food containing a significant amount of alcohol. While, in some cases, this behavioral decision can provide a survival advantage to the developing larvae, it can also lead to developmental and cognitive problems. Alcohol consumption can affect executive functions, episodic memory, and other brain function capacities. However, in the fruit fly, the initial cognitive effects of alcohol consumption have been shown to reverse upon persistent exposure to alcohol. Using an olfactory conditioning assay where an odorant is implemented as a conditioned stimulus and paired with a heat shock as an unconditioned stimulus, a previous study has shown that when exposed to a short acute dose of alcohol, Drosophila larvae can no longer learn this association. Interestingly, upon prolonged chronic alcohol exposure, larvae seem to successfully avoid the conditioned stimulus just as well as control alcohol-naive larvae, suggestive of alcohol-induced neuroadaptations. However, the mechanisms by which Drosophila adapt to the presence of alcohol remains unknown. In this study, we explore the transcriptional correlates of neuroadaptation in Drosophila larvae exposed to chronic alcohol to understand the genetic and cellular components responsible for this adaptation. For this, we employed RNA sequencing technology to evaluate differences in gene expression in the brain of larvae chronically exposed to alcohol. Our results suggest that alcohol-induced neuroadaptations are modulated by a diverse array of synaptic genes within the larval brain through a series of epigenetic modulators.Amanda Anqueira-GonzálezJenny P. Acevedo-GonzalezAirined Montes-MercadoClaudia Irizarry-HernándezNicolás L. Fuenzalida-UribeAlfredo GhezziFrontiers Media S.A.articleneuroadaptationalcohol use disorderDrosophila melanogastercognitiondifferential gene expressionRNA-seqNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENFrontiers in Behavioral Neuroscience, Vol 15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	neuroadaptation alcohol use disorder Drosophila melanogaster cognition differential gene expression RNA-seq Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	neuroadaptation alcohol use disorder Drosophila melanogaster cognition differential gene expression RNA-seq Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Amanda Anqueira-González Jenny P. Acevedo-Gonzalez Airined Montes-Mercado Claudia Irizarry-Hernández Nicolás L. Fuenzalida-Uribe Alfredo Ghezzi Transcriptional Correlates of Chronic Alcohol Neuroadaptation in Drosophila Larvae
description	When presented with the choice, Drosophila melanogaster females will often prefer to lay eggs on food containing a significant amount of alcohol. While, in some cases, this behavioral decision can provide a survival advantage to the developing larvae, it can also lead to developmental and cognitive problems. Alcohol consumption can affect executive functions, episodic memory, and other brain function capacities. However, in the fruit fly, the initial cognitive effects of alcohol consumption have been shown to reverse upon persistent exposure to alcohol. Using an olfactory conditioning assay where an odorant is implemented as a conditioned stimulus and paired with a heat shock as an unconditioned stimulus, a previous study has shown that when exposed to a short acute dose of alcohol, Drosophila larvae can no longer learn this association. Interestingly, upon prolonged chronic alcohol exposure, larvae seem to successfully avoid the conditioned stimulus just as well as control alcohol-naive larvae, suggestive of alcohol-induced neuroadaptations. However, the mechanisms by which Drosophila adapt to the presence of alcohol remains unknown. In this study, we explore the transcriptional correlates of neuroadaptation in Drosophila larvae exposed to chronic alcohol to understand the genetic and cellular components responsible for this adaptation. For this, we employed RNA sequencing technology to evaluate differences in gene expression in the brain of larvae chronically exposed to alcohol. Our results suggest that alcohol-induced neuroadaptations are modulated by a diverse array of synaptic genes within the larval brain through a series of epigenetic modulators.
format	article
author	Amanda Anqueira-González Jenny P. Acevedo-Gonzalez Airined Montes-Mercado Claudia Irizarry-Hernández Nicolás L. Fuenzalida-Uribe Alfredo Ghezzi
author_facet	Amanda Anqueira-González Jenny P. Acevedo-Gonzalez Airined Montes-Mercado Claudia Irizarry-Hernández Nicolás L. Fuenzalida-Uribe Alfredo Ghezzi
author_sort	Amanda Anqueira-González
title	Transcriptional Correlates of Chronic Alcohol Neuroadaptation in Drosophila Larvae
title_short	Transcriptional Correlates of Chronic Alcohol Neuroadaptation in Drosophila Larvae
title_full	Transcriptional Correlates of Chronic Alcohol Neuroadaptation in Drosophila Larvae
title_fullStr	Transcriptional Correlates of Chronic Alcohol Neuroadaptation in Drosophila Larvae
title_full_unstemmed	Transcriptional Correlates of Chronic Alcohol Neuroadaptation in Drosophila Larvae
title_sort	transcriptional correlates of chronic alcohol neuroadaptation in drosophila larvae
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/ac75cea8cf3f44208c1cf336b69acbfb
work_keys_str_mv	AT amandaanqueiragonzalez transcriptionalcorrelatesofchronicalcoholneuroadaptationindrosophilalarvae AT jennypacevedogonzalez transcriptionalcorrelatesofchronicalcoholneuroadaptationindrosophilalarvae AT airinedmontesmercado transcriptionalcorrelatesofchronicalcoholneuroadaptationindrosophilalarvae AT claudiairizarryhernandez transcriptionalcorrelatesofchronicalcoholneuroadaptationindrosophilalarvae AT nicolaslfuenzalidauribe transcriptionalcorrelatesofchronicalcoholneuroadaptationindrosophilalarvae AT alfredoghezzi transcriptionalcorrelatesofchronicalcoholneuroadaptationindrosophilalarvae
_version_	1718445169283432448

Transcriptional Correlates of Chronic Alcohol Neuroadaptation in Drosophila Larvae

Ejemplares similares