The noncoding small RNA SsrA is released by Vibrio fischeri and modulates critical host responses.

The regulatory noncoding small RNAs (sRNAs) of bacteria are key elements influencing gene expression; however, there has been little evidence that beneficial bacteria use these molecules to communicate with their animal hosts. We report here that the bacterial sRNA SsrA plays an essential role in th...

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Autores principales:	Silvia Moriano-Gutierrez, Clotilde Bongrand, Tara Essock-Burns, Leo Wu, Margaret J McFall-Ngai, Edward G Ruby
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2020
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ac7bc91751444a46b614a2d96e8fb34c
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spelling	oai:doaj.org-article:ac7bc91751444a46b614a2d96e8fb34c2021-12-02T19:54:18ZThe noncoding small RNA SsrA is released by Vibrio fischeri and modulates critical host responses.1544-91731545-788510.1371/journal.pbio.3000934https://doaj.org/article/ac7bc91751444a46b614a2d96e8fb34c2020-11-01T00:00:00Zhttps://doi.org/10.1371/journal.pbio.3000934https://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885The regulatory noncoding small RNAs (sRNAs) of bacteria are key elements influencing gene expression; however, there has been little evidence that beneficial bacteria use these molecules to communicate with their animal hosts. We report here that the bacterial sRNA SsrA plays an essential role in the light-organ symbiosis between Vibrio fischeri and the squid Euprymna scolopes. The symbionts load SsrA into outer membrane vesicles, which are transported specifically into the epithelial cells surrounding the symbiont population in the light organ. Although an SsrA-deletion mutant (ΔssrA) colonized the host to a normal level after 24 h, it produced only 2/10 the luminescence per bacterium, and its persistence began to decline by 48 h. The host's response to colonization by the ΔssrA strain was also abnormal: the epithelial cells underwent premature swelling, and host robustness was reduced. Most notably, when colonized by the ΔssrA strain, the light organ differentially up-regulated 10 genes, including several encoding heightened immune-function or antimicrobial activities. This study reveals the potential for a bacterial symbiont's sRNAs not only to control its own activities but also to trigger critical responses promoting homeostasis in its host. In the absence of this communication, there are dramatic fitness consequences for both partners.Silvia Moriano-GutierrezClotilde BongrandTara Essock-BurnsLeo WuMargaret J McFall-NgaiEdward G RubyPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 18, Iss 11, p e3000934 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Silvia Moriano-Gutierrez Clotilde Bongrand Tara Essock-Burns Leo Wu Margaret J McFall-Ngai Edward G Ruby The noncoding small RNA SsrA is released by Vibrio fischeri and modulates critical host responses.
description	The regulatory noncoding small RNAs (sRNAs) of bacteria are key elements influencing gene expression; however, there has been little evidence that beneficial bacteria use these molecules to communicate with their animal hosts. We report here that the bacterial sRNA SsrA plays an essential role in the light-organ symbiosis between Vibrio fischeri and the squid Euprymna scolopes. The symbionts load SsrA into outer membrane vesicles, which are transported specifically into the epithelial cells surrounding the symbiont population in the light organ. Although an SsrA-deletion mutant (ΔssrA) colonized the host to a normal level after 24 h, it produced only 2/10 the luminescence per bacterium, and its persistence began to decline by 48 h. The host's response to colonization by the ΔssrA strain was also abnormal: the epithelial cells underwent premature swelling, and host robustness was reduced. Most notably, when colonized by the ΔssrA strain, the light organ differentially up-regulated 10 genes, including several encoding heightened immune-function or antimicrobial activities. This study reveals the potential for a bacterial symbiont's sRNAs not only to control its own activities but also to trigger critical responses promoting homeostasis in its host. In the absence of this communication, there are dramatic fitness consequences for both partners.
format	article
author	Silvia Moriano-Gutierrez Clotilde Bongrand Tara Essock-Burns Leo Wu Margaret J McFall-Ngai Edward G Ruby
author_facet	Silvia Moriano-Gutierrez Clotilde Bongrand Tara Essock-Burns Leo Wu Margaret J McFall-Ngai Edward G Ruby
author_sort	Silvia Moriano-Gutierrez
title	The noncoding small RNA SsrA is released by Vibrio fischeri and modulates critical host responses.
title_short	The noncoding small RNA SsrA is released by Vibrio fischeri and modulates critical host responses.
title_full	The noncoding small RNA SsrA is released by Vibrio fischeri and modulates critical host responses.
title_fullStr	The noncoding small RNA SsrA is released by Vibrio fischeri and modulates critical host responses.
title_full_unstemmed	The noncoding small RNA SsrA is released by Vibrio fischeri and modulates critical host responses.
title_sort	noncoding small rna ssra is released by vibrio fischeri and modulates critical host responses.
publisher	Public Library of Science (PLoS)
publishDate	2020
url	https://doaj.org/article/ac7bc91751444a46b614a2d96e8fb34c
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The noncoding small RNA SsrA is released by Vibrio fischeri and modulates critical host responses.

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