An automated system for rapid non-destructive enumeration of growing microbes.

<h4>Background</h4>The power and simplicity of visual colony counting have made it the mainstay of microbiological analysis for more than 130 years. A disadvantage of the method is the long time required to generate visible colonies from cells in a sample. New rapid testing technologies...

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Autores principales: Roanna London, Julie Schwedock, Andrew Sage, Heather Valley, Jamie Meadows, Michael Waddington, Don Straus
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Publicado: Public Library of Science (PLoS) 2010
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Acceso en línea:https://doaj.org/article/ac7c57fdc2714afdbd3114858ba03ae8
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spelling oai:doaj.org-article:ac7c57fdc2714afdbd3114858ba03ae82021-11-25T06:26:52ZAn automated system for rapid non-destructive enumeration of growing microbes.1932-620310.1371/journal.pone.0008609https://doaj.org/article/ac7c57fdc2714afdbd3114858ba03ae82010-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20062794/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The power and simplicity of visual colony counting have made it the mainstay of microbiological analysis for more than 130 years. A disadvantage of the method is the long time required to generate visible colonies from cells in a sample. New rapid testing technologies generally have failed to maintain one or more of the major advantages of culture-based methods.<h4>Principal findings</h4>We present a new technology and platform that uses digital imaging of cellular autofluorescence to detect and enumerate growing microcolonies many generations before they become visible to the eye. The data presented demonstrate that the method preserves the viability of the microcolonies it detects, thus enabling generation of pure cultures for microbial identification. While visual colony counting detects Escherichia coli colonies containing about 5x10(6) cells, the new imaging method detects E. coli microcolonies when they contain about 120 cells and microcolonies of the yeast Candida albicans when they contain only about 12 cells. We demonstrate that digital imaging of microcolony autofluorescence detects a broad spectrum of prokaryotic and eukaryotic microbes and present a model for predicting the time to detection for individual strains. Results from the analysis of environmental samples from pharmaceutical manufacturing plants containing a mixture of unidentified microbes demonstrate the method's improved test turnaround times.<h4>Conclusion</h4>This work demonstrates a new technology and automated platform that substantially shortens test times while maintaining key advantages of the current methods.Roanna LondonJulie SchwedockAndrew SageHeather ValleyJamie MeadowsMichael WaddingtonDon StrausPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 1, p e8609 (2010)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Roanna London
Julie Schwedock
Andrew Sage
Heather Valley
Jamie Meadows
Michael Waddington
Don Straus
An automated system for rapid non-destructive enumeration of growing microbes.
description <h4>Background</h4>The power and simplicity of visual colony counting have made it the mainstay of microbiological analysis for more than 130 years. A disadvantage of the method is the long time required to generate visible colonies from cells in a sample. New rapid testing technologies generally have failed to maintain one or more of the major advantages of culture-based methods.<h4>Principal findings</h4>We present a new technology and platform that uses digital imaging of cellular autofluorescence to detect and enumerate growing microcolonies many generations before they become visible to the eye. The data presented demonstrate that the method preserves the viability of the microcolonies it detects, thus enabling generation of pure cultures for microbial identification. While visual colony counting detects Escherichia coli colonies containing about 5x10(6) cells, the new imaging method detects E. coli microcolonies when they contain about 120 cells and microcolonies of the yeast Candida albicans when they contain only about 12 cells. We demonstrate that digital imaging of microcolony autofluorescence detects a broad spectrum of prokaryotic and eukaryotic microbes and present a model for predicting the time to detection for individual strains. Results from the analysis of environmental samples from pharmaceutical manufacturing plants containing a mixture of unidentified microbes demonstrate the method's improved test turnaround times.<h4>Conclusion</h4>This work demonstrates a new technology and automated platform that substantially shortens test times while maintaining key advantages of the current methods.
format article
author Roanna London
Julie Schwedock
Andrew Sage
Heather Valley
Jamie Meadows
Michael Waddington
Don Straus
author_facet Roanna London
Julie Schwedock
Andrew Sage
Heather Valley
Jamie Meadows
Michael Waddington
Don Straus
author_sort Roanna London
title An automated system for rapid non-destructive enumeration of growing microbes.
title_short An automated system for rapid non-destructive enumeration of growing microbes.
title_full An automated system for rapid non-destructive enumeration of growing microbes.
title_fullStr An automated system for rapid non-destructive enumeration of growing microbes.
title_full_unstemmed An automated system for rapid non-destructive enumeration of growing microbes.
title_sort automated system for rapid non-destructive enumeration of growing microbes.
publisher Public Library of Science (PLoS)
publishDate 2010
url https://doaj.org/article/ac7c57fdc2714afdbd3114858ba03ae8
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