Nanoformulated Bioactive Compounds Derived from Different Natural Products Combat Pancreatic Cancer Cell Proliferation

Deena S Mousa,1 Ali H El-Far,2 Amna A Saddiq,3 Thangirala Sudha,4 Shaker A Mousa4 1Yale University, New Haven, CT, USA; 2Department of Biochemistry, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, Egypt; 3Faculty of Science, Department of Biology, University of Jeddah, Jeddah...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mousa DS, El-Far AH, Saddiq AA, Sudha T, Mousa SA
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	pancreatic cancer 3 3′-diindolylmethane ellagic acid plgapeg nanoparticles anti-angiogenesis chick chorioallantoic membrane model Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ac819cb4cb1a41968d772d5f5b6fc25d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ac819cb4cb1a41968d772d5f5b6fc25d
record_format	dspace
spelling	oai:doaj.org-article:ac819cb4cb1a41968d772d5f5b6fc25d2021-12-02T09:42:59ZNanoformulated Bioactive Compounds Derived from Different Natural Products Combat Pancreatic Cancer Cell Proliferation1178-2013https://doaj.org/article/ac819cb4cb1a41968d772d5f5b6fc25d2020-03-01T00:00:00Zhttps://www.dovepress.com/nanoformulated-bioactive-compounds-derived-from-different-natural-prod-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Deena S Mousa,1 Ali H El-Far,2 Amna A Saddiq,3 Thangirala Sudha,4 Shaker A Mousa4 1Yale University, New Haven, CT, USA; 2Department of Biochemistry, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, Egypt; 3Faculty of Science, Department of Biology, University of Jeddah, Jeddah 21589, Saudi Arabia; 4Pharmaceutical Research Institute, Albany College of Pharmacy and Health Sciences, Rensselaer, NY 12144, USACorrespondence: Shaker A MousaProfessor of Pharmacology, Albany College of Pharmacy and Health Sciences, Chairman and Executive Vice President, The Pharmaceutical Research Institute, One Discovery Drive, Rensselaer, New York 12144, USAEmail shaker.mousa@acphs.eduPurpose: This study was designed to determine the potential effect of nanoencapsulated bioactive compounds from different natural sources on human pancreatic cancer.Background: Pancreatic cancer carries the highest fatality rate among all human cancers because of its high metastatic potential and late presentation at the time of diagnosis. Hence there is a need for improved methods to prevent and treat it. Natural products, such as 3, 3′-diindolylmethane (DIM) and ellagic acid (EA) demonstrated anticancer efficacy against various cancer types. However, DIM is insoluble. Hence, using nanotechnology to encapsulate these compounds in combination with EA might improve their physical and chemical properties and their delivery to the cancer cells.Methods: Human pancreatic cancer cells, namely SUIT2-luciferase transfected, were used to examine the effects of DIM or EA and their nanoformulation in poly(lactic-co-glycolic acid) (PLGA) and poly(ethylene glycol) (PEG) [PLGA-PEG] nanoparticles (NPs) on SUIT2-luciferase cell viability/proliferation over 24 hrs. Additionally, effects on tumor weight and angiogenesis were determined using the chick chorioallantoic membrane (CAM) tumor implant model.Results: Both DIM and EA PLGA-PEG NPs resulted in rapid suppression of pancreatic cancer cell viability/proliferation within 24 hrs (P < 0.01), while the non-encapsulated DIM and EA did not show any significant effect on SUIT2 cancer cell viability or cell proliferation (MTT assay). In the CAM pancreatic cancer cell (SUIT2) implant model, results showed a greater suppression of tumor weight (P < 0.01), tumor cell viability, and tumor angiogenesis (P < 0.01) for DIM NPs and EA NPs and their combinations versus DIM or EA alone.Conclusion: Nanoformulation of DIM and EA resulted in a more effective suppression of pancreatic cancer cell viability, pancreatic tumor weight, implanted cancer cell viability, and tumor angiogenesis as compared with these bioactive compounds alone.Keywords: pancreatic cancer, 3, 3′-diindolylmethane, ellagic acid, PLGA-PEG nanoparticles, anti-angiogenesis, chick chorioallantoic membrane modelMousa DSEl-Far AHSaddiq AASudha TMousa SADove Medical Pressarticlepancreatic cancer33′-diindolylmethaneellagic acidplgapeg nanoparticlesanti-angiogenesischick chorioallantoic membrane modelMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 2259-2268 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	pancreatic cancer 3 3′-diindolylmethane ellagic acid plgapeg nanoparticles anti-angiogenesis chick chorioallantoic membrane model Medicine (General) R5-920
spellingShingle	pancreatic cancer 3 3′-diindolylmethane ellagic acid plgapeg nanoparticles anti-angiogenesis chick chorioallantoic membrane model Medicine (General) R5-920 Mousa DS El-Far AH Saddiq AA Sudha T Mousa SA Nanoformulated Bioactive Compounds Derived from Different Natural Products Combat Pancreatic Cancer Cell Proliferation
description	Deena S Mousa,1 Ali H El-Far,2 Amna A Saddiq,3 Thangirala Sudha,4 Shaker A Mousa4 1Yale University, New Haven, CT, USA; 2Department of Biochemistry, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, Egypt; 3Faculty of Science, Department of Biology, University of Jeddah, Jeddah 21589, Saudi Arabia; 4Pharmaceutical Research Institute, Albany College of Pharmacy and Health Sciences, Rensselaer, NY 12144, USACorrespondence: Shaker A MousaProfessor of Pharmacology, Albany College of Pharmacy and Health Sciences, Chairman and Executive Vice President, The Pharmaceutical Research Institute, One Discovery Drive, Rensselaer, New York 12144, USAEmail shaker.mousa@acphs.eduPurpose: This study was designed to determine the potential effect of nanoencapsulated bioactive compounds from different natural sources on human pancreatic cancer.Background: Pancreatic cancer carries the highest fatality rate among all human cancers because of its high metastatic potential and late presentation at the time of diagnosis. Hence there is a need for improved methods to prevent and treat it. Natural products, such as 3, 3′-diindolylmethane (DIM) and ellagic acid (EA) demonstrated anticancer efficacy against various cancer types. However, DIM is insoluble. Hence, using nanotechnology to encapsulate these compounds in combination with EA might improve their physical and chemical properties and their delivery to the cancer cells.Methods: Human pancreatic cancer cells, namely SUIT2-luciferase transfected, were used to examine the effects of DIM or EA and their nanoformulation in poly(lactic-co-glycolic acid) (PLGA) and poly(ethylene glycol) (PEG) [PLGA-PEG] nanoparticles (NPs) on SUIT2-luciferase cell viability/proliferation over 24 hrs. Additionally, effects on tumor weight and angiogenesis were determined using the chick chorioallantoic membrane (CAM) tumor implant model.Results: Both DIM and EA PLGA-PEG NPs resulted in rapid suppression of pancreatic cancer cell viability/proliferation within 24 hrs (P < 0.01), while the non-encapsulated DIM and EA did not show any significant effect on SUIT2 cancer cell viability or cell proliferation (MTT assay). In the CAM pancreatic cancer cell (SUIT2) implant model, results showed a greater suppression of tumor weight (P < 0.01), tumor cell viability, and tumor angiogenesis (P < 0.01) for DIM NPs and EA NPs and their combinations versus DIM or EA alone.Conclusion: Nanoformulation of DIM and EA resulted in a more effective suppression of pancreatic cancer cell viability, pancreatic tumor weight, implanted cancer cell viability, and tumor angiogenesis as compared with these bioactive compounds alone.Keywords: pancreatic cancer, 3, 3′-diindolylmethane, ellagic acid, PLGA-PEG nanoparticles, anti-angiogenesis, chick chorioallantoic membrane model
format	article
author	Mousa DS El-Far AH Saddiq AA Sudha T Mousa SA
author_facet	Mousa DS El-Far AH Saddiq AA Sudha T Mousa SA
author_sort	Mousa DS
title	Nanoformulated Bioactive Compounds Derived from Different Natural Products Combat Pancreatic Cancer Cell Proliferation
title_short	Nanoformulated Bioactive Compounds Derived from Different Natural Products Combat Pancreatic Cancer Cell Proliferation
title_full	Nanoformulated Bioactive Compounds Derived from Different Natural Products Combat Pancreatic Cancer Cell Proliferation
title_fullStr	Nanoformulated Bioactive Compounds Derived from Different Natural Products Combat Pancreatic Cancer Cell Proliferation
title_full_unstemmed	Nanoformulated Bioactive Compounds Derived from Different Natural Products Combat Pancreatic Cancer Cell Proliferation
title_sort	nanoformulated bioactive compounds derived from different natural products combat pancreatic cancer cell proliferation
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/ac819cb4cb1a41968d772d5f5b6fc25d
work_keys_str_mv	AT mousads nanoformulatedbioactivecompoundsderivedfromdifferentnaturalproductscombatpancreaticcancercellproliferation AT elfarah nanoformulatedbioactivecompoundsderivedfromdifferentnaturalproductscombatpancreaticcancercellproliferation AT saddiqaa nanoformulatedbioactivecompoundsderivedfromdifferentnaturalproductscombatpancreaticcancercellproliferation AT sudhat nanoformulatedbioactivecompoundsderivedfromdifferentnaturalproductscombatpancreaticcancercellproliferation AT mousasa nanoformulatedbioactivecompoundsderivedfromdifferentnaturalproductscombatpancreaticcancercellproliferation
_version_	1718398025690251264

Nanoformulated Bioactive Compounds Derived from Different Natural Products Combat Pancreatic Cancer Cell Proliferation

Ejemplares similares