Non-Deep Physiological Dormancy in Seed and Germination Requirements of <i>Lysimachia coreana</i> Nakai

This study aimed to determine the type of seed dormancy and to identify a suitable method of dormancy-breaking for an efficient seed viability test of <i>Lysimachia coreana</i> Nakai. To confirm the effect of gibberellic acid (GA<sub>3</sub>) on seed germination at different...

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Autores principales: Saeng Geul Baek, Jin Hyun Im, Myeong Ja Kwak, Cho Hee Park, Mi Hyun Lee, Chae Sun Na, Su Young Woo
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
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Acceso en línea:https://doaj.org/article/ac87e8c6e73b4c558388e1aedeb351b0
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Sumario:This study aimed to determine the type of seed dormancy and to identify a suitable method of dormancy-breaking for an efficient seed viability test of <i>Lysimachia coreana</i> Nakai. To confirm the effect of gibberellic acid (GA<sub>3</sub>) on seed germination at different temperatures, germination tests were conducted at 5, 15, 20, 25, 20/10, and 25/15 °C (12/12 h, light/dark), using 1% agar with 100, 250, and 500 mg·L<sup>−1</sup> GA<sub>3</sub>. Seeds were also stratified at 5 and 25/15 °C for 6 and 9 weeks, respectively, and then germinated at the same temperature. Seeds treated with GA<sub>3</sub> demonstrated an increased germination rate (GR) at all temperatures except 5 °C. The highest GR was 82.0% at 25/15 °C and 250 mg·L<sup>−1</sup> GA<sub>3</sub> (4.8 times higher than the control (14.0%)). Additionally, GR increased after cold stratification, whereas seeds did not germinate after warm stratification at all temperatures. After cold stratification, the highest GR was 56.0% at 25/15 °C, which was lower than the GR observed after GA<sub>3</sub> treatment. We hypothesized that <i>L. coreana</i> seeds have a non-deep physiological dormancy and concluded that 250 mg·L<sup>−1</sup> GA<sub>3</sub> treatment is more effective than cold stratification (9 weeks) for <i>L. coreana</i> seed-dormancy-breaking.