Short Communication: Detection and expression analysis of the bile salt hydrolase gene in Pediococcus and Lactobacillus

Abstract. Widodo, Nuratri Y, Sukarno AS, Ahyuningsih TD. 2020. Short Communication: Detection and expression analysis of the bile salt hydrolase gene in Pediococcus and Lactobacillus. Biodiversitas 21: 5901-5905. Bile salt hydrolase (BSH) has been considered an important enzyme that is produced in r...

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Autores principales:	Widodo Widodo, Nuratri Yana, Sukarno Ari Surya, Wahyuningsih Tutik Dwi
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	bile salt hydrolase, bsh, lactobacillus, pediococcus, probiotic Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ac8c165ae386435cacd9acd432f48404
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spelling	oai:doaj.org-article:ac8c165ae386435cacd9acd432f484042021-11-22T00:52:00ZShort Communication: Detection and expression analysis of the bile salt hydrolase gene in Pediococcus and Lactobacillus1412-033X2085-472210.13057/biodiv/d211255https://doaj.org/article/ac8c165ae386435cacd9acd432f484042020-12-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6976https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Widodo, Nuratri Y, Sukarno AS, Ahyuningsih TD. 2020. Short Communication: Detection and expression analysis of the bile salt hydrolase gene in Pediococcus and Lactobacillus. Biodiversitas 21: 5901-5905. Bile salt hydrolase (BSH) has been considered an important enzyme that is produced in response to bile stress. The bsh gene reportedly encodes BSH in lactic acid bacteria. Thus, the aim of this study was to detect and measure the changes in bsh gene expression in Pediococcus pentosaceus M103, Lactobacillus casei AP, Lactobacillus casei AG, and Lactobacillus paracasei M104. A BSH primer was used in amplifying the bacterial genomic DNA, and the obtained amplicon was then sequenced and analyzed in order to detect genes homologous to bsh. Changes in expression levels were measured in three concentrations of bile salts: 0.1%, 0.3%, and 0.5% (w/v). The BSH primer produced amplicons 850 to 950 kb in length. Amino acid sequences from the amplicons were determined to be similar to the product sequence of BSH. The level of expression of the bsh gene was altered in every concentration of bile salts in the growth medium with bsh genes from P. pentosaceus M103 were induced by bile salts at concentrations of 0.1% and 0.3% (w/v). We conclude that P. pentosaceus M103 may make suitable probiotic candidates that can tolerate conjugated bile acids.Widodo WidodoNuratri YanaSukarno Ari SuryaWahyuningsih Tutik DwiMBI & UNS Soloarticlebile salt hydrolase, bsh, lactobacillus, pediococcus, probioticBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	bile salt hydrolase, bsh, lactobacillus, pediococcus, probiotic Biology (General) QH301-705.5
spellingShingle	bile salt hydrolase, bsh, lactobacillus, pediococcus, probiotic Biology (General) QH301-705.5 Widodo Widodo Nuratri Yana Sukarno Ari Surya Wahyuningsih Tutik Dwi Short Communication: Detection and expression analysis of the bile salt hydrolase gene in Pediococcus and Lactobacillus
description	Abstract. Widodo, Nuratri Y, Sukarno AS, Ahyuningsih TD. 2020. Short Communication: Detection and expression analysis of the bile salt hydrolase gene in Pediococcus and Lactobacillus. Biodiversitas 21: 5901-5905. Bile salt hydrolase (BSH) has been considered an important enzyme that is produced in response to bile stress. The bsh gene reportedly encodes BSH in lactic acid bacteria. Thus, the aim of this study was to detect and measure the changes in bsh gene expression in Pediococcus pentosaceus M103, Lactobacillus casei AP, Lactobacillus casei AG, and Lactobacillus paracasei M104. A BSH primer was used in amplifying the bacterial genomic DNA, and the obtained amplicon was then sequenced and analyzed in order to detect genes homologous to bsh. Changes in expression levels were measured in three concentrations of bile salts: 0.1%, 0.3%, and 0.5% (w/v). The BSH primer produced amplicons 850 to 950 kb in length. Amino acid sequences from the amplicons were determined to be similar to the product sequence of BSH. The level of expression of the bsh gene was altered in every concentration of bile salts in the growth medium with bsh genes from P. pentosaceus M103 were induced by bile salts at concentrations of 0.1% and 0.3% (w/v). We conclude that P. pentosaceus M103 may make suitable probiotic candidates that can tolerate conjugated bile acids.
format	article
author	Widodo Widodo Nuratri Yana Sukarno Ari Surya Wahyuningsih Tutik Dwi
author_facet	Widodo Widodo Nuratri Yana Sukarno Ari Surya Wahyuningsih Tutik Dwi
author_sort	Widodo Widodo
title	Short Communication: Detection and expression analysis of the bile salt hydrolase gene in Pediococcus and Lactobacillus
title_short	Short Communication: Detection and expression analysis of the bile salt hydrolase gene in Pediococcus and Lactobacillus
title_full	Short Communication: Detection and expression analysis of the bile salt hydrolase gene in Pediococcus and Lactobacillus
title_fullStr	Short Communication: Detection and expression analysis of the bile salt hydrolase gene in Pediococcus and Lactobacillus
title_full_unstemmed	Short Communication: Detection and expression analysis of the bile salt hydrolase gene in Pediococcus and Lactobacillus
title_sort	short communication: detection and expression analysis of the bile salt hydrolase gene in pediococcus and lactobacillus
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/ac8c165ae386435cacd9acd432f48404
work_keys_str_mv	AT widodowidodo shortcommunicationdetectionandexpressionanalysisofthebilesalthydrolasegeneinpediococcusandlactobacillus AT nuratriyana shortcommunicationdetectionandexpressionanalysisofthebilesalthydrolasegeneinpediococcusandlactobacillus AT sukarnoarisurya shortcommunicationdetectionandexpressionanalysisofthebilesalthydrolasegeneinpediococcusandlactobacillus AT wahyuningsihtutikdwi shortcommunicationdetectionandexpressionanalysisofthebilesalthydrolasegeneinpediococcusandlactobacillus
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Short Communication: Detection and expression analysis of the bile salt hydrolase gene in Pediococcus and Lactobacillus

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